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Literature summary for 1.4.1.3 extracted from

  • Yoon, H.Y.; Lee, E.Y.; Cho, S.W.
    Cassette mutagenesis and photoaffinity labeling of adenine binding domain of ADP regulatory site within human glutamate dehydrogenase (2002), Biochemistry, 41, 6817-6823.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP 1 mM, pH 8.0, 3fold activation of wild-type enzyme, no significant actication of Tyr187 mutant enzymes Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y187E KM-values for NADH and 2-oxoglutareta are similar to wild-type values, about 4fold decrease of Vmax Homo sapiens
Y187G KM-values for NADH and 2-oxoglutareta are similar to wild-type values, about 4fold decrease of Vmax, no significant actication by ADP Homo sapiens
Y187M KM-values for NADH and 2-oxoglutareta are similar to wild-type values, about 4fold decrease of Vmax, no significant actication by ADP Homo sapiens
Y187S KM-values for NADH and 2-oxoglutareta are similar to wild-type values, about 4fold decrease of Vmax, no significant actication by ADP Homo sapiens
Y197R KM-values for NADH and 2-oxoglutareta are similar to wild-type values, about 4fold decrease of Vmax, no significant actication by ADP Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
NADH pH 8.0, 25┬░C, wild-type enzyme Homo sapiens
0.082
-
NADH pH 8.0, 25┬░C, mutant enzyme Y187E Homo sapiens
0.082
-
NADH pH 8.0, 25┬░C, mutant enzyme Y187R Homo sapiens
0.084
-
NADH pH 8.0, 25┬░C, mutant enzyme Y187M Homo sapiens
0.085
-
NADH pH 8.0, 25┬░C, mutant enzyme Y187G Homo sapiens
0.088
-
NADH pH 8.0, 25┬░C, mutant enzyme Y187S Homo sapiens
1.25
-
2-oxoglutarate pH 8.0, 25┬░C, wild-type enzyme Homo sapiens
1.29
-
2-oxoglutarate pH 8.0, 25┬░C, mutant enzyme Y187E Homo sapiens
1.31
-
2-oxoglutarate pH 8.0, 25┬░C, mutant enzyme Y187M Homo sapiens
1.44
-
2-oxoglutarate pH 8.0, 25┬░C, mutant enzyme Y187R Homo sapiens
1.45
-
2-oxoglutarate pH 8.0, 25┬░C, mutant enzyme Y187G Homo sapiens
1.46
-
2-oxoglutarate pH 8.0, 25┬░C, mutant enzyme Y187S Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
Homo sapiens L-glutamate + NAD+ + H2O
-
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Cofactor

Cofactor Comment Organism Structure
NADH
-
Homo sapiens