Cloned (Comment) | Organism |
---|---|
gene ksdD, phylogenetic analysis, expression in Bacillus subtilis strain 168 with 18fold higher activity compared to wild-type Mycobacterium neoaurum strain JC-12 | Mycolicibacterium neoaurum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + FAD | Mycolicibacterium neoaurum | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | Mycolicibacterium neoaurum JC-12 | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium neoaurum | - |
gene ksdD | - |
Mycolicibacterium neoaurum JC-12 | - |
gene ksdD | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.75 | - |
recombinant enzyme expressed in Bacillus subtilis, pH 7.0, 30°C | Mycolicibacterium neoaurum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + FAD | - |
Mycolicibacterium neoaurum | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol | Mycolicibacterium neoaurum | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | - |
Mycolicibacterium neoaurum JC-12 | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol | Mycolicibacterium neoaurum JC-12 | 1,4-androstadiene-3,17-dione + FADH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid-DELTA1-dehydrogenase | - |
Mycolicibacterium neoaurum |
KsdD | - |
Mycolicibacterium neoaurum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycolicibacterium neoaurum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycolicibacterium neoaurum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | putative N-terminal FAD-binding motif in KsdDM is GSG(A/G)(A/G)(A/G)X17E | Mycolicibacterium neoaurum |
General Information | Comment | Organism |
---|---|---|
metabolism | the 3-ketosteroid-DELTA1-dehydrogenase is involved in steroid metabolism catalyzing the transformation of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione specifically | Mycolicibacterium neoaurum |