Application | Comment | Organism |
---|---|---|
analysis | protocol to measure enzyme kinetic parameters based on isothermal titration calorimetry. Presence of dimethyl sulfoxide at 10%, v/v and Triton X-100 at 0.5%, v/v seems to facilitate the substrate binding process with a small decrease in KM value | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
expression of His-tagged enzyme | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 10% v/v dimethyl sulfoxide and 0.5% v/vTriton X-100, which seem to facilitate the substrate binding process with a small decrease in Km, enzyme-substrate complex, pseudo-first-order kinetics, thermodynamics, overview | Trypanosoma cruzi | |
0.00856 | - |
(S)-dihydroorotate | assay based on isothermal titration calorimetry, pH 8.0, 25°C | Trypanosoma cruzi | |
0.0086 | - |
L-dihydroorotate | pH not specified in the publication, temperature not specified in the publication | Trypanosoma cruzi | |
0.12 | - |
fumarate | pH not specified in the publication, temperature not specified in the publication | Trypanosoma cruzi | |
0.12 | - |
fumarate | assay based on isothermal titration calorimetry, pH 8.0, 25°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Trypanosoma cruzi | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
- |
Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihydroorotate + fumarate | Trypanosoma cruzi | DHODH catalyzes a coupled redox reaction in which dihydroorotate is oxidized to orotate and fumarate is reduced to succinate | orotate + succinate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | presence of dimethyl sulfoxide at 10%, v/v and Triton X-100 at 0.5%, v/v during the assay seems to facilitate the substrate binding process with a small decrease in KM value | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + fumarate | - |
Trypanosoma cruzi | orotate + succinate | - |
? | |
L-dihydroorotate + fumarate | - |
Trypanosoma cruzi | orotate + succinate | - |
? | |
L-dihydroorotate + fumarate | DHODH catalyzes a coupled redox reaction in which dihydroorotate is oxidized to orotate and fumarate is reduced to succinate | Trypanosoma cruzi | orotate + succinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
DHODH | - |
Trypanosoma cruzi |
More | a family 2 DHOD | Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Trypanosoma cruzi |