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show all sequences of 1.3.5.6

Contribution of two zeta-carotene desaturases to the poly-cis desaturation pathway in the cyanobacterium Nostoc PCC 7120

Breitenbach, J.; Bruns, M.; Sandmann, G.; Arch. Microbiol. 195, 491-498 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene crtQa, expressionin Escherichia coli, gene crtQb, expression of CrtQB in Escherichia coli strain JM101
Nostoc sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7,9,9'-tricis-neurosporene + a quinone
Nostoc sp.
catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb
7,9,7',9'-tetracis-lycopene + a quinol
-
-
?
9,9'-dicis-zeta-carotene + a quinone
Nostoc sp.
catalyzed by zeta-carotene desaturases CrtQa and CrtQb
7,9,9'-tricis-neurosporene + a quinol
-
-
?
additional information
Nostoc sp.
strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nostoc sp.
-
contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, encoded by genes crtQa and crtQb
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,9,9'-tricis-neurosporene + a quinone
catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb
724142
Nostoc sp.
7,9,7',9'-tetracis-lycopene + a quinol
-
-
-
?
9,9'-dicis-zeta-carotene + a quinone
catalyzed by zeta-carotene desaturases CrtQa and CrtQb
724142
Nostoc sp.
7,9,9'-tricis-neurosporene + a quinol
-
-
-
?
additional information
strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene
724142
Nostoc sp.
?
-
-
-
-
additional information
zeta-carotene desaturase CrtQb converts (9Z)-zeta-carotene efficiently into (7Z,9Z)-neurosporene but stops at this stage without substantial lycopene formation. (all-E)-zeta-carotene cannot be converted by CrtQb. Similarly to (9Z)-zeta-carotene, the (all-E)-isomer is efficiently converted by CrtQa, substrate specificities, overview
724142
Nostoc sp.
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
tightly bound to zeta-carotene desaturase CrtQb
Nostoc sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
gene crtQa, expressionin Escherichia coli, gene crtQb, expression of CrtQB in Escherichia coli strain JM101
Nostoc sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
tightly bound to zeta-carotene desaturase CrtQb
Nostoc sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7,9,9'-tricis-neurosporene + a quinone
Nostoc sp.
catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb
7,9,7',9'-tetracis-lycopene + a quinol
-
-
?
9,9'-dicis-zeta-carotene + a quinone
Nostoc sp.
catalyzed by zeta-carotene desaturases CrtQa and CrtQb
7,9,9'-tricis-neurosporene + a quinol
-
-
?
additional information
Nostoc sp.
strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7,9,9'-tricis-neurosporene + a quinone
catalyzed by zeta-carotene desaturase CrtQa, very poorly by CrtQb
724142
Nostoc sp.
7,9,7',9'-tetracis-lycopene + a quinol
-
-
-
?
9,9'-dicis-zeta-carotene + a quinone
catalyzed by zeta-carotene desaturases CrtQa and CrtQb
724142
Nostoc sp.
7,9,9'-tricis-neurosporene + a quinol
-
-
-
?
additional information
strain PCC 7120 contains two completely unrelated zeta-carotene desaturases CrtQa and CrtQb, which differ in their preferred utilization of zeta-carotene Z isomers. CrtQa con­verts zeta-carotene isomers that are poorly metabolized by CrtQb and CrtQa still possesses the Z to E isomerase function of the ancestral desaturase CrtI. CrtQb is an enzyme with one molecule of tightly bound FAD and acts as a dehydrogenase transferring hydrogen to oxidized plastoquinone. CrtQb yields only trace amounts of 7,9-Z neurosporene and 7,9,7',9'-Z lycopene
724142
Nostoc sp.
?
-
-
-
-
additional information
zeta-carotene desaturase CrtQb converts (9Z)-zeta-carotene efficiently into (7Z,9Z)-neurosporene but stops at this stage without substantial lycopene formation. (all-E)-zeta-carotene cannot be converted by CrtQb. Similarly to (9Z)-zeta-carotene, the (all-E)-isomer is efficiently converted by CrtQa, substrate specificities, overview
724142
Nostoc sp.
?
-
-
-
-
Other publictions for EC 1.3.5.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724142
Breitenbach
Contribution of two zeta-carot ...
Nostoc sp.
Arch. Microbiol.
195
491-498
2013
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Kim
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Citrus unshiu
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1339-1349
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660319
Breitenbach
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Gentiana lutea
Planta
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785-793
2005
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712351
Wang
Alteration of product specific ...
Rhodobacter sphaeroides
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276
41161-41164
2001
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710300
Josse
A plastid terminal oxidase ass ...
Capsicum annuum, Solanum lycopersicum
Plant Physiol.
123
1427-1436
2000
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708367
Breitenbach
Catalytic properties of an exp ...
Capsicum annuum
Eur. J. Biochem.
265
376-383
1999
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