Activating Compound | Comment | Organism | Structure |
---|---|---|---|
decyl-plastoquinone | enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate | Capsicum annuum | |
decyl-ubiquinone | enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate | Capsicum annuum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Capsicum annuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
zeta-carotene | pH 7.8, 28°C | Capsicum annuum | |
0.009 | - |
Neurosporene | pH 7.8, 28°C | Capsicum annuum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Capsicum annuum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
neurosporene + a quinone | Capsicum annuum | - |
lycopene + a quinol | - |
? | |
zeta-carotene + 2 quinone | Capsicum annuum | zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers | lycopene + 2 quinol | - |
? | |
zeta-carotene + a quinone | Capsicum annuum | - |
neurosporene + a quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Capsicum annuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
neurosporene + a quinone | - |
Capsicum annuum | lycopene + a quinol | - |
? | |
neurosporene + decyl-plastoquinone | - |
Capsicum annuum | lycopene + decyl-plastoquinol | - |
? | |
neurosporene + decyl-ubiquinone | - |
Capsicum annuum | lycopene + decyl-ubiquinone | - |
? | |
zeta-carotene + 2 decyl-plastoquinone | zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers | Capsicum annuum | lycopene + 2 decyl-plastoquinol | - |
? | |
zeta-carotene + 2 decyl-ubiquinone | zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers | Capsicum annuum | lycopene + 2 decyl-ubiquinone | - |
? | |
zeta-carotene + 2 quinone | zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers | Capsicum annuum | lycopene + 2 quinol | - |
? | |
zeta-carotene + a quinone | - |
Capsicum annuum | neurosporene + a quinol | - |
? | |
zeta-carotene + decyl-plastoquinone | - |
Capsicum annuum | neurosporene + decyl-plastoquinol | - |
? | |
zeta-carotene + decyl-ubiquinone | - |
Capsicum annuum | neurosporene + decyl-ubiquinone | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Capsicum annuum |
Synonyms | Comment | Organism |
---|---|---|
zeta-carotene desaturase | - |
- |
zeta-carotene desaturase | - |
Capsicum annuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Capsicum annuum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of the dinucleotides FAD, NADP+ or NAD+ alone or the latter in combination with ATP results in a negligible increase in zeta-carotene desaturase activity | Capsicum annuum | |
quinone | - |
Capsicum annuum |