BRENDA - Enzyme Database show
show all sequences of 1.3.5.6

Catalytic properties of an expressed and purified higher plant type zeta-carotene desaturase from Capsicum annuum

Breitenbach, J.; Kuntz, M.; Takaichi, S.; Sandmann, G.; Eur. J. Biochem. 265, 376-383 (1999)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
decyl-plastoquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
decyl-ubiquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Capsicum annuum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0084
-
zeta-carotene
pH 7.8, 28°C
Capsicum annuum
0.009
-
Neurosporene
pH 7.8, 28°C
Capsicum annuum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Capsicum annuum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
neurosporene + a quinone
Capsicum annuum
-
lycopene + a quinol
-
-
?
zeta-carotene + 2 quinone
Capsicum annuum
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
lycopene + 2 quinol
-
-
?
zeta-carotene + a quinone
Capsicum annuum
-
neurosporene + a quinol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Capsicum annuum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Capsicum annuum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
neurosporene + a quinone
-
708367
Capsicum annuum
lycopene + a quinol
-
-
-
?
neurosporene + decyl-plastoquinone
-
708367
Capsicum annuum
lycopene + decyl-plastoquinol
-
-
-
?
neurosporene + decyl-ubiquinone
-
708367
Capsicum annuum
lycopene + decyl-ubiquinone
-
-
-
?
zeta-carotene + 2 decyl-plastoquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-plastoquinol
-
-
-
?
zeta-carotene + 2 decyl-ubiquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-ubiquinone
-
-
-
?
zeta-carotene + 2 quinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 quinol
-
-
-
?
zeta-carotene + a quinone
-
708367
Capsicum annuum
neurosporene + a quinol
-
-
-
?
zeta-carotene + decyl-plastoquinone
-
708367
Capsicum annuum
neurosporene + decyl-plastoquinol
-
-
-
?
zeta-carotene + decyl-ubiquinone
-
708367
Capsicum annuum
neurosporene + decyl-ubiquinone
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Capsicum annuum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Capsicum annuum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Capsicum annuum
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
addition of the dinucleotides FAD, NADP+ or NAD+ alone or the latter in combination with ATP results in a negligible increase in zeta-carotene desaturase activity
Capsicum annuum
quinone
-
Capsicum annuum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
decyl-plastoquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
decyl-ubiquinone
enhances zeta-carotene desaturase activity significantly and increases the ratio of lycopene to neurosporene among the reaction products. Quinones other than decyl-plastoquinone and decyl-ubiquinone do not stimulate
Capsicum annuum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Capsicum annuum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
addition of the dinucleotides FAD, NADP+ or NAD+ alone or the latter in combination with ATP results in a negligible increase in zeta-carotene desaturase activity
Capsicum annuum
quinone
-
Capsicum annuum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0084
-
zeta-carotene
pH 7.8, 28°C
Capsicum annuum
0.009
-
Neurosporene
pH 7.8, 28°C
Capsicum annuum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Capsicum annuum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
neurosporene + a quinone
Capsicum annuum
-
lycopene + a quinol
-
-
?
zeta-carotene + 2 quinone
Capsicum annuum
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
lycopene + 2 quinol
-
-
?
zeta-carotene + a quinone
Capsicum annuum
-
neurosporene + a quinol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Capsicum annuum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
neurosporene + a quinone
-
708367
Capsicum annuum
lycopene + a quinol
-
-
-
?
neurosporene + decyl-plastoquinone
-
708367
Capsicum annuum
lycopene + decyl-plastoquinol
-
-
-
?
neurosporene + decyl-ubiquinone
-
708367
Capsicum annuum
lycopene + decyl-ubiquinone
-
-
-
?
zeta-carotene + 2 decyl-plastoquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-plastoquinol
-
-
-
?
zeta-carotene + 2 decyl-ubiquinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 decyl-ubiquinone
-
-
-
?
zeta-carotene + 2 quinone
zeta-carotene desaturase has no preference for certain isomers. The nature of the isomers formed during catalysis depends strictly on the isomeric composition of the substrate. Different lycopene isomers are formed, including substantial amounts of the all-trans form, together with 7,7',9,9'-tetracis prolycopene via the corresponding neurosporene isomers
708367
Capsicum annuum
lycopene + 2 quinol
-
-
-
?
zeta-carotene + a quinone
-
708367
Capsicum annuum
neurosporene + a quinol
-
-
-
?
zeta-carotene + decyl-plastoquinone
-
708367
Capsicum annuum
neurosporene + decyl-plastoquinol
-
-
-
?
zeta-carotene + decyl-ubiquinone
-
708367
Capsicum annuum
neurosporene + decyl-ubiquinone
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Capsicum annuum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
28
-
assay at
Capsicum annuum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Capsicum annuum
Other publictions for EC 1.3.5.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724142
Breitenbach
Contribution of two zeta-carot ...
Nostoc sp.
Arch. Microbiol.
195
491-498
2013
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1
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5
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725182
Alferez
Modification of carotenoid lev ...
Citrus sinensis, Citrus sinensis valencia
J. Agric. Food Chem.
61
3082-3089
2013
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1
2
2
1
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742721
Alferez
Modification of carotenoid le ...
Citrus sinensis, Citrus sinensis L. Osbeck
J. Agric. Food Chem.
61
3082-3089
2013
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1
1
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3
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1
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1
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1
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743489
Fantini
Dissection of tomato lycopene ...
Solanum lycopersicum
Plant Physiol.
163
986-998
2013
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1
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1
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1
1
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711020
Stickforth
Structural and kinetics proper ...
Rubrivivax gelatinosus
Arch. Biochem. Biophys.
505
118-122
2011
-
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1
-
12
-
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6
1
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-
4
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2
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4
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1
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1
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1
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12
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6
1
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4
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4
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1
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1
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-
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2
2
-
-
-
716179
Yan
Cloning and expression analysi ...
Carica papaya, Carica papaya Dwarf solo
Mol. Biol. Rep.
38
785-791
2011
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1
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6
1
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2
2
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725561
Li
Cloning and characterization o ...
Auxenochlorella protothecoides, Auxenochlorella protothecoides CS-41
J. Biomed. Biotechnol.
2011
731542
2011
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1
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1
1
1
1
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712989
Cong
cDNA cloning and expression an ...
Triticum aestivum, Triticum aestivum Chinese Spring
Mol. Biol. Rep.
37
3351-3361
2010
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1
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4
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1
1
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715214
Ye
-
Analysis of an essential carot ...
Dunaliella salina, Dunaliella salina UTEX LB 200
J. Agric. Food Chem.
58
11477-11482
2010
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1
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2
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1
1
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716498
Kim
Transformation of carotenoid b ...
Citrus unshiu
Plant Cell Rep.
29
1339-1349
2010
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1
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1
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660319
Breitenbach
zeta-Carotene cis isomers as p ...
Gentiana lutea
Planta
220
785-793
2005
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1
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712351
Wang
Alteration of product specific ...
Rhodobacter sphaeroides
J. Biol. Chem.
276
41161-41164
2001
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8
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8
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2
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710300
Josse
A plastid terminal oxidase ass ...
Capsicum annuum, Solanum lycopersicum
Plant Physiol.
123
1427-1436
2000
-
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-
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2
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6
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2
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2
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1
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1
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2
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2
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2
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2
2
2
2
-
-
708367
Breitenbach
Catalytic properties of an exp ...
Capsicum annuum
Eur. J. Biochem.
265
376-383
1999
2
-
1
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-
-
2
-
-
1
3
-
4
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1
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9
1
1
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1
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2
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2
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1
2
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2
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1
3
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1
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9
1
1
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1
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