Data extracted from this reference:
KM Value [mM]
0.0005
2-trans-octenoyl-CoA
reductase type I
Candida albicans
0.0011
2-cis-octenoyl-CoA
reductase type I
Candida albicans
0.0025
5-hydroxyundec-cis-2-enoyl-CoA
reductase type I
Candida albicans
0.003
5-hydroxyundec-cis-2-enoyl-CoA
reductase type II
Candida albicans
0.01
2-trans-octenoyl-CoA
reductase type II
Candida albicans
0.053
2-cis-octenoyl-CoA
reductase type II
Candida albicans
Molecular Weight [Da]
85000
reductase type II, gel filtration
Candida albicans
180000
reductase type I, gel filtration
Candida albicans
Natural Substrates/ Products (Substrates)
5-hydroxyundec-cis-2-enoyl-CoA + NADPH
Candida albicans
5-hydroxyundecanoyl-CoA + NADP+
?
additional information
Candida albicans
role in beta-oxidation of fatty acids
?
Organism
Candida albicans
reductase type I and II
Purification (Commentary)
reductase type I and II
Candida albicans
Specific Activity [micromol/min/mg]
additional information
Candida albicans
Substrates and Products (Substrate)
2-cis-octenoyl-CoA + NADPH
390854
Candida albicans
octanoyl-CoA + NADP+
?
2-trans-octenoyl-CoA + NAD(P)H
390854
Candida albicans
octanoyl-CoA + NADP+
?
5-hydroxyundec-cis-2-enoyl-CoA + NADPH
390854
Candida albicans
5-hydroxyundecanoyl-CoA + NADP+
?
additional information
role in beta-oxidation of fatty acids
390854
Candida albicans
?
Temperature Stability [°C]
50
reductase type I: 30 min, 50% loss of activity, reductase type II: no activity with 5 hydroxyundec-cis-2-enoyl-CoA, up to 80% activity with cis or trans-oct-2-enoyl-CoA
Candida albicans
60
reductase type I: 3 min, 80% loss of activity
Candida albicans
pH Optimum
6.5
8.5
depending on substrate and type of reductase
Candida albicans
Cofactor
NADPH
inactive with NADH
Candida albicans
Cofactor (protein specific)
NADPH
inactive with NADH
Candida albicans
KM Value [mM] (protein specific)
0.0005
2-trans-octenoyl-CoA
reductase type I
Candida albicans
0.0011
2-cis-octenoyl-CoA
reductase type I
Candida albicans
0.0025
5-hydroxyundec-cis-2-enoyl-CoA
reductase type I
Candida albicans
0.003
5-hydroxyundec-cis-2-enoyl-CoA
reductase type II
Candida albicans
0.01
2-trans-octenoyl-CoA
reductase type II
Candida albicans
0.053
2-cis-octenoyl-CoA
reductase type II
Candida albicans
Molecular Weight [Da] (protein specific)
85000
reductase type II, gel filtration
Candida albicans
180000
reductase type I, gel filtration
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
5-hydroxyundec-cis-2-enoyl-CoA + NADPH
Candida albicans
5-hydroxyundecanoyl-CoA + NADP+
?
additional information
Candida albicans
role in beta-oxidation of fatty acids
?
Purification (Commentary) (protein specific)
reductase type I and II
Candida albicans
Specific Activity [micromol/min/mg] (protein specific)
additional information
Candida albicans
Substrates and Products (Substrate) (protein specific)
2-cis-octenoyl-CoA + NADPH
390854
Candida albicans
octanoyl-CoA + NADP+
?
2-trans-octenoyl-CoA + NAD(P)H
390854
Candida albicans
octanoyl-CoA + NADP+
?
5-hydroxyundec-cis-2-enoyl-CoA + NADPH
390854
Candida albicans
5-hydroxyundecanoyl-CoA + NADP+
?
additional information
role in beta-oxidation of fatty acids
390854
Candida albicans
?
Temperature Stability [°C] (protein specific)
50
reductase type I: 30 min, 50% loss of activity, reductase type II: no activity with 5 hydroxyundec-cis-2-enoyl-CoA, up to 80% activity with cis or trans-oct-2-enoyl-CoA
Candida albicans
60
reductase type I: 3 min, 80% loss of activity
Candida albicans
pH Optimum (protein specific)
6.5
8.5
depending on substrate and type of reductase
Candida albicans
Other publictions for EC 1.3.1.8
712769
Sugishima
Crystal structures of the subs ...
Arabidopsis thaliana
J. Mol. Biol.
402
879-891
2010
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685980
Rennison
Enhanced acyl-CoA dehydrogenas ...
Rattus norvegicus
Cardiovasc. Res.
79
331-340
2008
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689745
Erb
Synthesis of C5-dicarboxylic a ...
Rhodobacter sphaeroides
Proc. Natl. Acad. Sci. USA
104
10631-10636
2007
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673619
Park
Silencing of NbECR encoding a ...
Nicotiana benthamiana
FEBS Lett.
579
4459-4464
2005
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2
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1
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6
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1
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656737
Li
Crotonyl-coenzyme A reductase ...
Streptomyces collinus
Microbiology
150
3463-3472
2004
-
2
1
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-
-
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1
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3
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390872
Sargueil
High metabolism and subsequent ...
Mus musculus
Neurosci. Lett.
273
29-32
1999
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-
-
-
-
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1
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2
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2
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1
4
-
3
-
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-
-
-
-
390873
Lessire
Study of the 3-hydroxy-eicosan ...
Allium ampeloprasum
Plant Physiol.
119
1009-1015
1999
-
-
-
-
-
-
-
-
1
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1
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3
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1
1
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2
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1
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2
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-
-
-
-
390869
Mukherjee
Inactivation of enoyl-CoA redu ...
Columba sp.
J. Enzyme Inhib.
13
217-228
1998
-
-
-
-
-
-
1
-
-
-
-
-
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2
-
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1
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1
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1
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1
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-
-
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390868
Mukherjee
Evidence for the essential his ...
Columba sp.
J. Enzyme Inhib.
11
209-216
1997
-
-
-
-
-
-
1
-
-
-
-
1
-
1
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-
-
-
1
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-
-
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-
-
1
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1
-
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1
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1
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-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
390759
Laguna
Action of Ebselen on rat hepat ...
Rattus norvegicus
Arch. Biochem. Biophys.
269
272-283
1989
-
-
-
-
-
-
1
-
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-
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-
1
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1
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2
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1
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2
-
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-
-
-
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-
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-
-
-
-
-
390758
Nagi
Dual action of 2-decynoyl coen ...
Rattus norvegicus
Arch. Biochem. Biophys.
267
1-12
1988
-
-
-
-
-
-
1
-
1
-
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1
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1
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2
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1
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2
-
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-
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-
-
-
-
-
-
390757
Nagi
Site of inhibition of rat live ...
Rattus norvegicus
J. Biol. Chem.
261
13598-13605
1986
-
-
-
-
-
-
1
-
1
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1
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1
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1
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1
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1
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-
-
-
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-
-
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-
-
-
-
390753
Prasad
Solubilization and purificatio ...
Rattus norvegicus
Arch. Biochem. Biophys.
237
535-544
1985
-
-
-
-
-
-
1
4
1
-
-
-
-
2
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1
-
-
1
1
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8
-
1
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1
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1
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1
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4
1
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1
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1
1
-
8
-
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-
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-
-
-
-
-
390870
Cvetanovic
Purification and characterizat ...
Bos taurus
Biochem. J.
227
49-56
1985
-
-
-
-
-
-
5
-
2
-
4
-
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2
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1
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2
5
-
8
1
1
1
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1
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5
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8
1
1
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390810
Inui
Fatty acid synthesis in mitoch ...
Euglena gracilis
Eur. J. Biochem.
142
121-126
1984
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3
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1
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4
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9
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9
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390849
Kikuchi
Purification of NADPH-dependen ...
Mycolicibacterium smegmatis
J. Biochem.
96
841-848
1984
-
-
-
-
-
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3
2
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2
1
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4
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1
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6
1
1
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2
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390754
Nagi
Biochemical properties of shor ...
Rattus norvegicus
Arch. Biochem. Biophys.
226
50-64
1983
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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390755
Prasad
Kinetic evidence for two separ ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
113
659-665
1983
-
-
-
-
-
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1
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1
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1
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5
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1
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5
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-
390756
Cinti
Evidence for a second microsom ...
Rattus norvegicus
J. Biol. Chem.
257
14333-14340
1982
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1
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390752
Bernert
The isolation of acyl-CoA deri ...
Rattus norvegicus
Biochim. Biophys. Acta
573
436-442
1979
-
-
-
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1
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1
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390850
Strom
-
Activation and inhibition of c ...
Bos taurus
J. Biol. Chem.
254
8159-8162
1979
1
-
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9
-
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1
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1
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4
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1
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9
4
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1
-
1
1
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1
1
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390851
Strom
Enoyl coenzyme A reduction by ...
Bos taurus
J. Biol. Chem.
254
8153-8158
1979
-
-
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1
2
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2
1
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1
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1
1
1
8
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1
1
8
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390751
Bernert
An analysis of partial reactio ...
Rattus norvegicus
J. Biol. Chem.
252
6736-6744
1977
1
-
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2
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1
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1
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1
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6
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1
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1
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1
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1
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6
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1
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-
390852
Maitra
Crotonyl coenzyme A reductase ...
Bos taurus
J. Biol. Chem.
249
111-117
1974
1
-
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1
3
-
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2
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1
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1
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1
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1
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1
1
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1
-
-
-
-
-
-
-
-
-
390853
Ishidate
Induction of NADPH enoyl-coA r ...
Candida albicans
J. Biochem.
76
1139-1142
1974
-
-
-
-
-
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-
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2
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2
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1
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-
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-
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-
390854
Ishidate
Separation and some characteri ...
Candida albicans
Chem. Pharm. Bull.
22
2685-2691
1974
-
-
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-
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6
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2
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390855
Mizugaki
Stereospecific hydrogen transf ...
Rattus norvegicus
J. Biochem.
74
691-696
1973
-
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1
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390856
Mizugaki
Possible role of NADPH-depende ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
50
48-53
1973
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3
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390857
Podack
On the mechanism of malonyl-Co ...
Rattus norvegicus
Biochim. Biophys. Acta
280
235-247
1972
-
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390858
Seubert
On the mechanism of malonyl-Co ...
Rattus norvegicus
Biochim. Biophys. Acta
164
498-517
1968
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