BRENDA - Enzyme Database
show all sequences of 1.3.1.75

Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis

Canniffe, D.P.; Chidgey, J.W.; Hunter, C.N.; Biochem. J. 462, 433-440 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene bciA
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
8-vinyl protochlorophyllide a + NADPH + H+
Rhodobacter sphaeroides
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
protochlorophyllide a + NADP+
-
-
?
8-vinyl protochlorophyllide a + NADPH + H+
Rhodobacter sphaeroides DSM 158
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
protochlorophyllide a + NADP+
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
Rhodobacter sphaeroides
8V Chlide is the preferred substrate for BciA, with high substrate specificity
chlorophyllide a + NADP+
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
Rhodobacter sphaeroides DSM 158
8V Chlide is the preferred substrate for BciA, with high substrate specificity
chlorophyllide a + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodobacter sphaeroides
Q3IXP7
-
-
Rhodobacter sphaeroides DSM 158
Q3IXP7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
8-vinyl protochlorophyllide a + NADPH + H+
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
744270
Rhodobacter sphaeroides
protochlorophyllide a + NADP+
-
-
-
?
8-vinyl protochlorophyllide a + NADPH + H+
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
744270
Rhodobacter sphaeroides DSM 158
protochlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
8V Chlide is the preferred substrate for BciA, with high substrate specificity
744270
Rhodobacter sphaeroides
chlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
preferred substrate with high substrate specificity
744270
Rhodobacter sphaeroides
chlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
8V Chlide is the preferred substrate for BciA, with high substrate specificity
744270
Rhodobacter sphaeroides DSM 158
chlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
preferred substrate with high substrate specificity
744270
Rhodobacter sphaeroides DSM 158
chlorophyllide a + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
8V reductase
-
Rhodobacter sphaeroides
8VR
-
Rhodobacter sphaeroides
BciA
-
Rhodobacter sphaeroides
C8-vinyl reductase
-
Rhodobacter sphaeroides
nucleoside-diphosphate-sugar epimerase
UniProt
Rhodobacter sphaeroides
RSP_3070
-
Rhodobacter sphaeroides
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rhodobacter sphaeroides
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bciA
Rhodobacter sphaeroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
8-vinyl protochlorophyllide a + NADPH + H+
Rhodobacter sphaeroides
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
protochlorophyllide a + NADP+
-
-
?
8-vinyl protochlorophyllide a + NADPH + H+
Rhodobacter sphaeroides DSM 158
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
protochlorophyllide a + NADP+
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
Rhodobacter sphaeroides
8V Chlide is the preferred substrate for BciA, with high substrate specificity
chlorophyllide a + NADP+
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
Rhodobacter sphaeroides DSM 158
8V Chlide is the preferred substrate for BciA, with high substrate specificity
chlorophyllide a + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
8-vinyl protochlorophyllide a + NADPH + H+
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
744270
Rhodobacter sphaeroides
protochlorophyllide a + NADP+
-
-
-
?
8-vinyl protochlorophyllide a + NADPH + H+
is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide
744270
Rhodobacter sphaeroides DSM 158
protochlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
8V Chlide is the preferred substrate for BciA, with high substrate specificity
744270
Rhodobacter sphaeroides
chlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
preferred substrate with high substrate specificity
744270
Rhodobacter sphaeroides
chlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
8V Chlide is the preferred substrate for BciA, with high substrate specificity
744270
Rhodobacter sphaeroides DSM 158
chlorophyllide a + NADP+
-
-
-
?
C8-vinyl chlorophyllide a + NADPH + H+
preferred substrate with high substrate specificity
744270
Rhodobacter sphaeroides DSM 158
chlorophyllide a + NADP+
-
-
-
?
Expression
Organism
Commentary
Expression
Rhodobacter sphaeroides
generation of an enzyme BciA knockout mutant and recombinant expression of Synechocystis sp. PCC6803 gene bciB in the mutant, gene bciB is unable to restore the accumulation of pigments containing groups reduced at the C8 position
additional information
General Information
General Information
Commentary
Organism
evolution
two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB
Rhodobacter sphaeroides
metabolism
the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment. Although BciA demonstrates flexible specificity, biosynthetic heterogeneity does not occur in this organism when its preferred substrate is present, implying that, in this case, the biosynthetic pathway is linear
Rhodobacter sphaeroides
General Information (protein specific)
General Information
Commentary
Organism
evolution
two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB
Rhodobacter sphaeroides
metabolism
the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment. Although BciA demonstrates flexible specificity, biosynthetic heterogeneity does not occur in this organism when its preferred substrate is present, implying that, in this case, the biosynthetic pathway is linear
Rhodobacter sphaeroides
Expression (protein specific)
Organism
Commentary
Expression
Rhodobacter sphaeroides
generation of an enzyme BciA knockout mutant and recombinant expression of Synechocystis sp. PCC6803 gene bciB in the mutant, gene bciB is unable to restore the accumulation of pigments containing groups reduced at the C8 position
additional information
Other publictions for EC 1.3.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745221
Chen
Two unrelated 8-vinyl reducta ...
Acaryochloris marina, Acaryochloris marina MBIC11017
J. Bacteriol.
198
1393-1400
2016
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1
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4
4
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746442
Tsukatani
Rhodobacter sphaeroides mutan ...
Rhodobacter sphaeroides
Sci. Rep.
5
9741
2015
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1
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7
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1
1
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736444
Harada
Chlorophyllide a oxidoreductas ...
Rhodobacter sphaeroides, Rhodopseudomonas palustris, Rhodopseudomonas palustris J002, Rhodobacter sphaeroides J001
J. Biol. Chem.
289
12716-12726
2014
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2
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4
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744270
Canniffe
Elucidation of the preferred ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides DSM 158
Biochem. J.
462
433-440
2014
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1
2
2
1
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745981
Harada
Isolation and structural dete ...
Chlorobaculum tepidum, Chlorobaculum tepidum TLS
Photosyn. Res.
121
13-23
2014
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1
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746037
Ito
Evolution of a new chlorophyl ...
Phaeodactylum tricornutum, Arabidopsis thaliana
Plant Cell Physiol.
55
593-603
2014
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2
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3
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1
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2
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7
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1
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1
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4
4
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746260
Tikh
A tale of two reductases ext ...
Rhodobacter sphaeroides, Chlorobaculum tepidum, Chlorobaculum tepidum TLS
PLoS ONE
9
e89734
2014
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2
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2
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4
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8
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2
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7
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12
2
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2
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2
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2
2
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2
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2
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7
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2
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2
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1
1
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746105
Wang
One divinyl reductase reduces ...
Zea mays, Cucumis sativus, Oryza sativa, Arabidopsis thaliana
Plant Physiol.
161
521-534
2013
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4
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1
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10
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12
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6
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14
2
13
4
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4
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1
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10
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14
2
4
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9
9
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726217
Wang
Divinyl chlorophyll(ide) a can ...
Oryza sativa
Plant Physiol.
153
994-1003
2010
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2
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13
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1
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1
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1
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739277
Nagata
The major route for chlorophyl ...
Arabidopsis thaliana
Plant Cell Physiol.
48
1803-1808
2007
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667191
Kolossov
Chloroplast biogenesis 92: In ...
Cucumis sativus
Anal. Biochem.
348
192-197
2006
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639381
Kolossov
Chloroplast biogenesis 84: sol ...
Cucumis sativus, Hordeum vulgare
Anal. Biochem.
295
214-219
2001
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639380
Parham
Chloroplast biogenesis 72: a [ ...
Cucumis sativus, Hordeum vulgare, Zea mays
Anal. Biochem.
231
164-169
1995
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639379
Parham
Chloroplast biogenesis: [4-vin ...
Cucumis sativus, Hordeum vulgare
Biochemistry
31
8460-8464
1992
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639378
Tripathy
Chloroplast biogenesis 60. Con ...
Cucumis sativus, Hordeum vulgare
Plant Physiol.
87
89-94
1988
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