Information on EC 1.3.1.75 - 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

for references in articles please use BRENDA:EC1.3.1.75
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.3.1.75
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RECOMMENDED NAME
GeneOntology No.
3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protochlorophyllide a + NADP+ = 3,8-divinyl protochlorophyllide a + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bacteriochlorophyll a biosynthesis
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bacteriochlorophyll c biosynthesis
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bacteriochlorophyll d biosynthesis
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bacteriochlorophyll e biosynthesis
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chlorophyll a biosynthesis I
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chlorophyll a biosynthesis II
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chlorophyll metabolism
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Porphyrin and chlorophyll metabolism
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
protochlorophyllide-a:NADP+ C-81-oxidoreductase
The enzyme, found in higher plants, green algae, and some phototrophic bacteria, is involved in the production of monovinyl versions of (bacterio)chlorophyll pigments from their divinyl precursors. It can also act on 3,8-divinyl chlorophyllide a. cf. EC 1.3.7.13, 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin).
CAS REGISTRY NUMBER
COMMENTARY hide
143590-98-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rifampicin-resistant mutant strain J001 derived from wild-type strain 2.4.1, gene bchXYZ
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Manually annotated by BRENDA team
a rifampicin-resistant variant of wild-type strain CGA009, gene bciB
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Manually annotated by BRENDA team
a rifampicin-resistant variant of wild-type strain CGA009, gene bciB
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-divinyl chlorophyllide a + NADPH + H+
2-vinyl-4-ethyl chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
bacteriochlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyll a + NADPH + H+
3-monovinyl chlorophyll a + NADP+
show the reaction diagram
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?
3,8-divinyl chlorophyll a + NADPH + H+
3-vinyl-chlorophyll a + NADP+
show the reaction diagram
3,8-divinyl chlorophyll a + NADPH + H+
chlorophyll a + NADP+
show the reaction diagram
3,8-divinyl chlorophyllide a + NADPH + H+
3-monovinyl chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyllide a + NADPH + H+
3-vinyl-chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyllide a + NADPH + H+
chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
3,8-divinyl protochlorophyllide a + NADPH + H+
3-vinyl-protochlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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8-vinyl protochlorophyllide a + NADPH + H+
protochlorophyllide a + NADP+
show the reaction diagram
C8-vinyl chlorophyllide a + NADPH + H+
chlorophyllide a + NADP+
show the reaction diagram
C8-vinyl chlorophyllide a + NADPH + H+
clorophyllide a + NADP+
show the reaction diagram
chlorophyllide a + NADP+
divinyl chlorophyllide a + NADPH + H+
show the reaction diagram
divinyl chlorophyllide a + NADPH + H+
monovinyl chlorophyllide a + NADP+
show the reaction diagram
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,4-divinyl chlorophyllide a + NADPH + H+
2-vinyl-4-ethyl chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl bacteriochlorophyllide a + NADPH + H+
bacteriochlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyll a + NADPH + H+
3-vinyl-chlorophyll a + NADP+
show the reaction diagram
3,8-divinyl chlorophyll a + NADPH + H+
chlorophyll a + NADP+
show the reaction diagram
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very low activity
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?
3,8-divinyl chlorophyllide a + NADPH + H+
3-monovinyl chlorophyllide a + NADP+
show the reaction diagram
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major substrate
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?
3,8-divinyl chlorophyllide a + NADPH + H+
3-vinyl-chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyllide a + NADPH + H+
chlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
3,8-divinyl protochlorophyllide a + NADPH + H+
3-vinyl-protochlorophyllide a + NADP+
show the reaction diagram
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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8-vinyl protochlorophyllide a + NADPH + H+
protochlorophyllide a + NADP+
show the reaction diagram
C8-vinyl chlorophyllide a + NADPH + H+
clorophyllide a + NADP+
show the reaction diagram
chlorophyllide a + NADP+
divinyl chlorophyllide a + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
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NADH is not an appropriate reductant
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.17
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partially purified enzyme
additional information
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assay development
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
7.5
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
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about half-maximal activity at pH 5.5 and pH 8.5, no activity below pH 5.2 and above pH 11.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 25
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
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about half-maximal activity at 10C and 50C
20 - 40
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more than 50% activity between 20 and 40C, while no activity is found at 50C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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complete inactivation after 2 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes the membrane-bound enzyme, it has no effect on the solubilized enzyme
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80 C, partially purified enzyme, stable for several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21 cells; recombinant protein expressed in Escherichia coli
gene bchXYZa, cloning and DNA and amino acid sequence determination and analysis, the plasmid carrying genes bchXYZ is used for complementation fo bchXYZ-deficient mutant strains of Rhodobacter sphaeroides, Rhodobacter palustris, Chlorobium tepidum, and Rhodobacter castenholzii
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gene bciA or nmrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, nmrA is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
gene bciA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with the enzymes of the biosynthetic pathway, engineered pathway design for the heterologous production of bacteriochlorophyll in the non-photosynthetic host Escherichia coli, overview. RSBciA is active, albeit not in vivo with the engineered pathway. Subcloning in Escherichia coli strain JM109
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gene bciA, recombinant expression in Escherichia coli, coexpression with the enzymes of the biosynthetic pathway, engineered pathway design for the heterologous production of bacteriochlorophyll in the non-photosynthetic host Escherichia coli, overview. CTBciA is capable of reducing the C8-vinyl group of several different intermediates in the BChl pathway. Subcloning in Escherichia coli strain JM109
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gene DVR or PCB2, a single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons
gene DVR, a single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons
gene DVR, DNA and amino acid sequence determination and analysis, sequence comparisons
gene DVR, phylogenetic analysis
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gene DVR, phylogenetic analysis, recombinant expression of NADPH-dependent DVR in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
generation of an enzyme BciA knockout mutant and recombinant expression of Synechocystis sp. PCC6803 gene bciB in the mutant, gene bciB is unable to restore the accumulation of pigments containing groups reduced at the C8 position
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information