BRENDA - Enzyme Database show
show all sequences of 1.3.1.74

Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin DELTA13-reductase (LTB4 12-HD/PGR) responsible for the reduction of a double-bond of the alpha,beta-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670

Itoh, K.; Yamamoto, K.; Adachi, M.; Kosaka, T.; Tanaka, Y.; Xenobiotica 38, 249-263 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ammonium sulfate
stimulates activity
Rattus norvegicus
CH3COO- NH4+
stimulates activity
Rattus norvegicus
CH3COONa
stimulates activity
Rattus norvegicus
Na2S2O5
stimulates activity
Rattus norvegicus
Na2SO4
stimulates activity
Rattus norvegicus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Top10 cells
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
15-keto prostaglandin F2alpha
43.06% residual activity at 0.1 mM
Rattus norvegicus
benzylidene acetophenone
21.09% residual activity at 0.5 mM
Rattus norvegicus
dicoumarol
11.45% residual activity at 0.1 mM
Rattus norvegicus
indomethacin
14.22% residual activity at 0.1 mM
Rattus norvegicus
additional information
finasteride has no inhibitory effect
Rattus norvegicus
quercitrin
13.14% residual activity at 0.1 mM
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid
at 30°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
stimulates activity
Rattus norvegicus
K+
stimulates activity
Rattus norvegicus
Li+
stimulates activity
Rattus norvegicus
Mg2+
stimulates activity
Rattus norvegicus
Mn2+
stimulates activity
Rattus norvegicus
Na+
stimulates activity
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
SDS-PAGE
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
2',5'-ADP Sepharose 4B column chromatography, Resource Q column chromatography, and HiLoad Superdex gel filtration
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0054
-
cytosol extract
Rattus norvegicus
0.803
-
after 150fold purification
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid + NADPH
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid is also known as CS-670
690204
Rattus norvegicus
2-[4-[(2-oxocyclohexyl)methyl]phenyl]propanoic acid + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ammonium sulfate
stimulates activity
Rattus norvegicus
CH3COO- NH4+
stimulates activity
Rattus norvegicus
CH3COONa
stimulates activity
Rattus norvegicus
Na2S2O5
stimulates activity
Rattus norvegicus
Na2SO4
stimulates activity
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Top10 cells
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
15-keto prostaglandin F2alpha
43.06% residual activity at 0.1 mM
Rattus norvegicus
benzylidene acetophenone
21.09% residual activity at 0.5 mM
Rattus norvegicus
dicoumarol
11.45% residual activity at 0.1 mM
Rattus norvegicus
indomethacin
14.22% residual activity at 0.1 mM
Rattus norvegicus
additional information
finasteride has no inhibitory effect
Rattus norvegicus
quercitrin
13.14% residual activity at 0.1 mM
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid
at 30°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
stimulates activity
Rattus norvegicus
K+
stimulates activity
Rattus norvegicus
Li+
stimulates activity
Rattus norvegicus
Mg2+
stimulates activity
Rattus norvegicus
Mn2+
stimulates activity
Rattus norvegicus
Na+
stimulates activity
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
2',5'-ADP Sepharose 4B column chromatography, Resource Q column chromatography, and HiLoad Superdex gel filtration
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0054
-
cytosol extract
Rattus norvegicus
0.803
-
after 150fold purification
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid + NADPH
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid is also known as CS-670
690204
Rattus norvegicus
2-[4-[(2-oxocyclohexyl)methyl]phenyl]propanoic acid + NADP+
-
-
-
?
Other publictions for EC 1.3.1.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725462
Yamauchi
NADPH-dependent reductases inv ...
Arabidopsis thaliana, Cucumis sativus
J. Biol. Chem.
286
6999-7009
2011
-
-
2
-
-
-
-
15
3
-
2
-
-
6
-
-
2
-
-
2
-
-
34
2
-
-
-
15
-
-
-
4
-
-
-
-
-
3
6
-
-
-
-
-
-
23
3
-
3
-
-
-
-
3
-
3
-
-
34
3
-
-
-
23
-
-
-
-
-
-
-
-
13
20
726133
Mano
-
Accumulation of lipid peroxide ...
Arabidopsis thaliana
Plant Biotechnol.
27
193-197
2010
-
-
1
-
-
-
-
-
-
-
-
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1
-
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-
-
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1
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1
1
-
-
-
697015
Zhang
-
Molecular cloning and characte ...
Artemisia annua
Botany
87
643-649
2009
-
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
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-
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1
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4
1
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1
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2
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1
2
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2
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1
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1
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4
1
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-
1
-
-
-
-
-
-
-
-
-
689624
Papdi
Functional identification of A ...
Arabidopsis thaliana
Plant Physiol.
147
528-542
2008
1
-
-
-
-
-
-
-
-
-
-
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-
1
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2
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1
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2
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-
-
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-
-
-
-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
5
-
1
-
-
-
6
1
1
6
1
-
-
3
-
-
1
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
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-
5
-
1
1
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-
6
-
1
1
6
1
-
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1
-
1
2
-
1
-
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-
-
-
-
-
-
-
-
-
-
674632
Yu
Nrf2-mediated induction of cyt ...
Rattus norvegicus
J. Biol. Chem.
281
26245-26252
2006
-
-
1
-
-
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-
2
-
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4
-
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1
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-
1
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2
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3
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1
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1
1
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2
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1
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1
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2
-
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3
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675296
Gong
Investigating the role of ster ...
Homo sapiens
J. Med. Chem.
49
2593-2599
2006
-
-
1
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4
-
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1
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1
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4
-
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1
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1
1
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4
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1
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-
4
-
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-
-
-
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-
-
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-
676581
Mano
Protection against photooxidat ...
Arabidopsis thaliana
Plant Physiol.
139
1773-1783
2005
-
-
1
-
-
-
-
6
2
-
-
-
-
4
-
-
-
-
-
1
-
-
6
-
-
-
-
7
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
655242
Dick
NADPH alkenal/one oxidoreducta ...
Rattus norvegicus
Clin. Cancer Res.
10
1492-1499
2004
-
-
1
-
-
-
3
3
-
-
-
3
-
3
-
-
-
-
-
-
3
-
7
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
3
-
-
-
3
-
-
-
-
-
-
3
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656210
Dick
The catalytic and kinetic mech ...
Rattus norvegicus
J. Biol. Chem.
279
17269-17277
2004
-
-
-
-
-
-
4
5
-
1
-
1
-
3
-
-
-
1
-
-
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-
4
-
1
-
-
-
1
1
-
2
1
-
-
-
-
-
2
-
-
-
-
4
1
5
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
636484
Mano
The NADPH:quinone oxidoreducta ...
Arabidopsis thaliana
Plant Cell Physiol.
43
1445-1455
2002
-
-
1
-
-
-
-
9
-
-
-
1
-
1
-
-
1
-
-
-
-
-
12
-
-
-
-
9
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
9
-
-
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
9
1
1
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
3
4
-
-
-
1
-
1
-
-
1
-
-
1
-
1
12
-
1
-
-
4
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
4
-
-
-
1
-
-
-
1
-
1
-
1
12
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
390628
Stoffel
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
356
385-390
1975
-
-
-
-
-
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3
-
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1
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1
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1
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2
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3
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1
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1
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2
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