BRENDA - Enzyme Database
show all sequences of 1.3.1.74

The catalytic and kinetic mechanisms of NADPH-dependent alkenal/one oxidoreductase

Dick, R.A.; Kensler, T.W.; J. Biol. Chem. 279, 17269-17277 (2004)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-nonanone
product inhibition, competitive versus trans-3-nonen-2-one
Rattus norvegicus
additional information
no inhibition by 9,10-phenanthroline
Rattus norvegicus
NADP+
product inhibition, competitive versus NADPH
Rattus norvegicus
Zn2+
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
ordered Theorell-Chance kinetic mechanism, steady-state kinetics, detailed analysis, kinetic isotopic effects
Rattus norvegicus
0.004
-
NADPH
recombinant enzyme, pH 7.2, 25°C, with substrate trans-2-nonenal
Rattus norvegicus
0.028
-
trans-3-nonen-2-one
recombinant enzyme, pH 7.2, 25°C
Rattus norvegicus
0.032
-
NADPH
recombinant enzyme, pH 7.2, 25°C, with substrate trans-3-nonen-2-one
Rattus norvegicus
0.078
-
trans-2-nonenal
recombinant enzyme, pH 7.2, 25°C
Rattus norvegicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
enzyme contains no Zn2+
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Rattus norvegicus
phase2/antioxidative enzyme
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
a n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H + H+
catalytic and kinetic mechanism
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
phase2/antioxidative enzyme
656210
Rattus norvegicus
?
-
-
-
-
additional information
regio- and stereochemistry of hydride transfer, overview
656210
Rattus norvegicus
?
-
-
-
-
trans-2-nonenal + NAD(P)H
-
656210
Rattus norvegicus
nonanal + NAD(P)+
-
-
-
?
trans-3-nonen-2-one + NAD(P)H
-
656210
Rattus norvegicus
2-nonanone + NAD(P)+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
alkenal/one oxidoreductase
-
Rattus norvegicus
AOR
-
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
pH-profile
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
low activity, 20fold higher activity with NADPH
Rattus norvegicus
NADPH
dependent on, beta-specific
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
product inhibition kinetics
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
low activity, 20fold higher activity with NADPH
Rattus norvegicus
NADPH
dependent on, beta-specific
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-nonanone
product inhibition, competitive versus trans-3-nonen-2-one
Rattus norvegicus
additional information
no inhibition by 9,10-phenanthroline
Rattus norvegicus
NADP+
product inhibition, competitive versus NADPH
Rattus norvegicus
Zn2+
-
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
product inhibition kinetics
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
ordered Theorell-Chance kinetic mechanism, steady-state kinetics, detailed analysis, kinetic isotopic effects
Rattus norvegicus
0.004
-
NADPH
recombinant enzyme, pH 7.2, 25°C, with substrate trans-2-nonenal
Rattus norvegicus
0.028
-
trans-3-nonen-2-one
recombinant enzyme, pH 7.2, 25°C
Rattus norvegicus
0.032
-
NADPH
recombinant enzyme, pH 7.2, 25°C, with substrate trans-3-nonen-2-one
Rattus norvegicus
0.078
-
trans-2-nonenal
recombinant enzyme, pH 7.2, 25°C
Rattus norvegicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
enzyme contains no Zn2+
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Rattus norvegicus
phase2/antioxidative enzyme
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
phase2/antioxidative enzyme
656210
Rattus norvegicus
?
-
-
-
-
additional information
regio- and stereochemistry of hydride transfer, overview
656210
Rattus norvegicus
?
-
-
-
-
trans-2-nonenal + NAD(P)H
-
656210
Rattus norvegicus
nonanal + NAD(P)+
-
-
-
?
trans-3-nonen-2-one + NAD(P)H
-
656210
Rattus norvegicus
2-nonanone + NAD(P)+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
pH-profile
Rattus norvegicus
Other publictions for EC 1.3.1.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725462
Yamauchi
NADPH-dependent reductases inv ...
Arabidopsis thaliana, Cucumis sativus
J. Biol. Chem.
286
6999-7009
2011
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2
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15
3
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2
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6
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2
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2
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34
2
6
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15
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4
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3
6
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23
3
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3
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3
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3
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34
3
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23
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-
13
20
726133
Mano
-
Accumulation of lipid peroxide ...
Arabidopsis thaliana
Plant Biotechnol.
27
193-197
2010
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1
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1
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1
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1
1
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697015
Zhang
-
Molecular cloning and characte ...
Artemisia annua
Botany
87
643-649
2009
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1
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2
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1
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1
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1
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4
1
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2
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2
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1
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1
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4
1
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1
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689624
Papdi
Functional identification of A ...
Arabidopsis thaliana
Plant Physiol.
147
528-542
2008
1
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1
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690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
5
-
1
-
-
-
6
1
1
6
1
-
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3
-
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1
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1
2
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4
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5
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674632
Yu
Nrf2-mediated induction of cyt ...
Rattus norvegicus
J. Biol. Chem.
281
26245-26252
2006
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1
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2
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4
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1
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3
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675296
Gong
Investigating the role of ster ...
Homo sapiens
J. Med. Chem.
49
2593-2599
2006
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1
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4
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1
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1
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1
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1
1
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4
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1
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4
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676581
Mano
Protection against photooxidat ...
Arabidopsis thaliana
Plant Physiol.
139
1773-1783
2005
-
-
1
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-
6
2
-
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-
4
-
-
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-
1
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6
-
1
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7
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1
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1
1
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6
2
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1
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6
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7
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-
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-
-
-
655242
Dick
NADPH alkenal/one oxidoreducta ...
Rattus norvegicus
Clin. Cancer Res.
10
1492-1499
2004
-
-
1
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3
3
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3
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3
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3
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7
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2
1
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1
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1
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1
1
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3
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3
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3
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3
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7
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1
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1
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656210
Dick
The catalytic and kinetic mech ...
Rattus norvegicus
J. Biol. Chem.
279
17269-17277
2004
-
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4
5
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1
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1
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3
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1
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4
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2
1
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1
1
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1
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2
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4
1
5
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1
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1
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4
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1
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1
1
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636484
Mano
The NADPH:quinone oxidoreducta ...
Arabidopsis thaliana
Plant Cell Physiol.
43
1445-1455
2002
-
-
1
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-
9
-
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1
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1
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1
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12
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9
1
1
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1
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1
1
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9
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1
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1
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12
-
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9
1
1
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390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
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1
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3
4
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1
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1
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1
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1
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1
12
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1
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4
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1
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1
1
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3
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4
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1
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1
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1
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1
12
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1
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4
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-
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390628
Stoffel
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
356
385-390
1975
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