Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | required for activation | Faecalicatena orotica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Methylorotate | 2 mM, 50% inhibition of reduction of orotate | Faecalicatena orotica | |
isopropyl hydantoin | - |
Faecalicatena orotica | |
Na3PO4 | 0.2 M, at pH 6.5, 20% inhibition | Faecalicatena orotica | |
NaCl | 0.2 M, 55% inhibition | Faecalicatena orotica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
Orotate | - |
Faecalicatena orotica | |
0.13 | - |
5-Fluoroorotate | - |
Faecalicatena orotica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Faecalicatena orotica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Faecalicatena orotica |
Storage Stability | Organism |
---|---|
above 20°C, 0.2 M phosphate buffer, fast loss of activity | Faecalicatena orotica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + NAD+ | - |
Faecalicatena orotica | orotate + NADH + H+ | - |
r | |
5-fluorodihydroorotate + NAD+ | more active substrate for reverse reaction than orotate | Faecalicatena orotica | 5-fluoroorotate + NADH + H+ | - |
r | |
additional information | not: uracil, cytosine, 5-methylcytosine, thymine as substrates | Faecalicatena orotica | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values not per enzyme molecule, but per mol of flavin | Faecalicatena orotica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
maximal activity | Faecalicatena orotica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, ratio FAD to FMN 1:1 | Faecalicatena orotica | |
flavin | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica | |
FMN | flavoprotein, ratio FAD to FMN 1:1 | Faecalicatena orotica | |
additional information | contains 2 mol of iron per mol of enzyme | Faecalicatena orotica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
isopropyl hydantoin | - |
Faecalicatena orotica |