Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | KCN, reversible | Moorella thermoacetica | |
KCN | CO reverses cyanide inhibition, but promotes reaction with methyl iodide | Moorella thermoacetica | |
additional information | not affected by propyl iodide, methyl iodide, carbon tetrachloride,or metal chelators; not inhibited by propyl iodide, methyl iodide, carbon tetrachloride, EDTA or nitrilotriacetate | Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | - |
Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
410000 | - |
gel filtration | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + electron acceptor | Moorella thermoacetica | ferredoxin and a membrane-bound b-type cytochrome are considered to be the native electron carriers | ? | - |
? | |
CO + H2O + oxidized cytochrome b | Moorella thermoacetica | membrane-bound b-type, native electron carrier | CO2 + reduced cytochrome b | - |
? | |
CO + H2O + oxidized ferredoxin | Moorella thermoacetica | - |
CO2 + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Moorella thermoacetica | - |
formerly Clostridium thermoaceticum | - |
Oxidation Stability | Organism |
---|---|
enzymatic activity is destroyed by air, a low amount of activity still is present after 2 h exposure to air | Moorella thermoacetica |
Purification (Comment) | Organism |
---|---|
- |
Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
method for in situ assay | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + cytochrome b | - |
Moorella thermoacetica | CO2 + reduced cytochrome b | - |
? | |
CO + H2O + cytochrome c3 | cytochrome 3 from Desulfovibrio vulgaris | Moorella thermoacetica | CO2 + reduced cytochrome c3 | - |
? | |
CO + H2O + electron acceptor | ferredoxin and a membrane-bound b-type cytochrome are considered to be the native electron carriers | Moorella thermoacetica | ? | - |
? | |
CO + H2O + ferredoxin | active with ferredoxin obtained from Clostridium thermoaceticum, no activity with ferredoxin from spinach | Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + FMN | - |
Moorella thermoacetica | CO2 + FMNH2 | - |
? | |
CO + H2O + methyl viologen | no activity with FAD, NAD+ and NADP+ | Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
CO + H2O + oxidized cytochrome b | membrane-bound b-type, native electron carrier | Moorella thermoacetica | CO2 + reduced cytochrome b | - |
? | |
CO + H2O + oxidized cytochrome c3 | Desulfovibrio vulgaris cytochrome c3 | Moorella thermoacetica | CO2 + reduced cytochrome c3 | - |
? | |
CO + H2O + oxidized ferredoxin | - |
Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + oxidized ferredoxin | ferredoxin from Clostridium pasteurianum is also a substrate | Moorella thermoacetica | CO2 + reduced ferredoxin | - |
? | |
CO + H2O + oxidized flavodoxin | - |
Moorella thermoacetica | CO2 + reduced flavodoxin | - |
? | |
CO + H2O + oxidized methyl viologen | - |
Moorella thermoacetica | CO2 + reduced methyl viologen | - |
? | |
additional information | spinach ferredoxin, FAD, NAD+ and NADP+ are not reduced | Moorella thermoacetica | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.6 | - |
- |
Moorella thermoacetica |
10.6 | - |
metyl viologen as substrate | Moorella thermoacetica |