Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Hydrogenobacter thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus | involved in reductive TCA cycle | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | Hydrogenobacter thermophilus TK-6 / IAM 12695 | involved in reductive TCA cycle | succinyl-CoA + CO2 + reduced ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 | D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | D3DIA1 and D3DIA2 and D3DIA3 and D3DIA0 and Q93RA0 | D3DIA1: subunit forA, D3DIA2: subunit forB, D3DIA3: subunit forG, D3DIA0: subunit forD, Q93RA0: subunit forE | - |
Purification (Comment) | Organism |
---|---|
- |
Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.34 | - |
70°C, pH not specified in the publication, recombinant enzyme, oxidative decarboxylation of 2-oxoglutarate | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CoA + oxidized ferredoxin | - |
Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | involved in reductive TCA cycle | Hydrogenobacter thermophilus | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + oxidized ferredoxin | involved in reductive TCA cycle | Hydrogenobacter thermophilus TK-6 / IAM 12695 | succinyl-CoA + CO2 + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | five-subunit-structure: ForA, ForB, ForG, ForD, and ForE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
forDABGE | gene name, five-subunit-structure: ForA, ForB, ForG, ForD, and ForE | Hydrogenobacter thermophilus |
OGOR | - |
Hydrogenobacter thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Hydrogenobacter thermophilus |