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Information on EC 1.2.7.3 - 2-oxoglutarate synthase
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EC Tree
1.2.7.3 2-oxoglutarate synthaseIUBMB Comments
The enzyme contains thiamine diphosphate and two [4Fe-4S] clusters. Highly specific for 2-oxoglutarate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that oxidatively decarboxylate different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase and EC 1.2.7.7, 3-methyl-2-oxobutanoate dehydrogenase (ferredoxin).
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Word Map
- 1.2.7.3
-
tricarboxylic
- ferredoxins
-
hydrogen-oxidizing
- hydrogenobacter
- 2-oxoacids
-
pyruvate:ferredoxin
-
ferredoxin-dependent
-
2-oxoacid:ferredoxin
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
+
2
=
+
+
2
+
2
Synonyms
2-oxoglutarate:ferredoxin oxidoreductase, 2-oxoglutarate ferredoxin oxidoreductase, 2-oxoglutarate synthase, alpha-ketoglutarate synthase, alpha-ketoglutarate oxidoreductase, 2-oxoglutarate oxidoreductase, 2-ketoglutarate ferredoxin oxidoreductase, fordabge, oordabc, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-ketoglutarate ferredoxin oxidoreductase
-
-
-
-
2-oxoglutarate ferredoxin oxidoreductase
-
-
-
-
2-oxoglutarate oxidoreductase
2-oxoglutarate:ferredoxin 2-oxidoreductase (CoA-succinylating)
-
-
-
-
2-oxoglutarate:ferredoxin oxidoreductase
alpha-ketoglutarate oxidoreductase
alpha-ketoglutarate synthase
alpha-ketoglutarate-ferredoxin oxidoreductase
-
-
-
-
CoA-dependent 2-ketoglutarate oxidoreductase
EC 1.2.7.9
-
-
formerly
-
FOR enzyme
For(OGOR)
forDABGE
KGOR
KOR enzyme
Kor(OGOR)
Mmc1_1749
Mmc1_1750
OGOR
OOR
oorDABC
2-oxoglutarate:ferredoxin oxidoreductase
-
-
-
-
2-oxoglutarate:ferredoxin oxidoreductase
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
2-oxoglutarate:ferredoxin oxidoreductase
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
-
forDABGE
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
gene name, five-subunit-structure: ForA, ForB, ForG, ForD, and ForE
forDABGE
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
gene name, five-subunit-structure: ForA, ForB, ForG, ForD, and ForE
-
KGOR
-
-
-
-
Mmc1_1749
A0L8G4; A0L8G5
gene name, pyruvate flavodoxin/ferredoxin oxidoreductase domain protein
Mmc1_1749
A0L8G4; A0L8G5
gene name, pyruvate flavodoxin/ferredoxin oxidoreductase domain protein
-
OGOR
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-oxoglutarate + CoA + 2 oxidized ferredoxin = succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidative decarboxylation
-
-
-
-
redox reaction
-
-
-
-
reductive carboxylation
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
2-oxoglutarate:ferredoxin oxidoreductase (decarboxylating)
The enzyme contains thiamine diphosphate and two [4Fe-4S] clusters. Highly specific for 2-oxoglutarate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that oxidatively decarboxylate different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase and EC 1.2.7.7, 3-methyl-2-oxobutanoate dehydrogenase (ferredoxin).
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CAS REGISTRY NUMBER
COMMENTARY
37251-05-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized methyl viologen
succinyl-CoA + CO2 + reduced methyl viologen
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
A0L8G4; A0L8G5
-
-
-
r
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
A0L8G4; A0L8G5
-
-
-
r
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
O87870; Q8RJQ9
specific for 2-oxoglutarate
-
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates
-
-
?
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
pure enzyme does not utilize other 2-keto acids, such as pyruvate, phenylglyoxylate, indolepyruvate, 2-oxobutanoate or 2-oxoisovalerate, as substrates
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
-
-
r
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
-
-
r
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Q9AJM0; Q9AJL9
involved in carbon dioxide fixation via a reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
involved in reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Q9AJM0; Q9AJL9
activity of the recombinant enzyme with 2-oxobutyrate, 2-oxoisocaproate, 2-oxoisovalerate, oxalacetate, oxomalonate, pyruvate or phosphoenolpyruvate is less than 5% of the activity with 2-oxoglutarate
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
-
no activity with oxalacetate, oxomalonate, 2-oxoisocaproate, or phosphoenolpyruvate. The enzyme reacts with 2-oxobutyrate, pyruvate, and 2-oxoisovalerate at an activity level of less than 0.4 to 0.7% relative to that with 2-oxoglutarate. The enzyme uses 2-oxoglutarate, coenzyme A, and one of several electron acceptors in substrate amounts (ferredoxin isolated from Hydrogenobacter thermophilus, flavin adenine dinucleotide, flavin mononucleotide, or methyl viologen). NAD+, NADP+, and ferredoxins from Chlorella spp. and Clostridium pasteurianum are ineffective
-
-
r
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
no activity with oxalacetate, oxomalonate, 2-oxoisocaproate, or phosphoenolpyruvate. The enzyme reacts with 2-oxobutyrate, pyruvate, and 2-oxoisovalerate at an activity level of less than 0.4 to 0.7% relative to that with 2-oxoglutarate. The enzyme uses 2-oxoglutarate, coenzyme A, and one of several electron acceptors in substrate amounts (ferredoxin isolated from Hydrogenobacter thermophilus, flavin adenine dinucleotide, flavin mononucleotide, or methyl viologen). NAD+, NADP+, and ferredoxins from Chlorella spp. and Clostridium pasteurianum are ineffective
-
-
r
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
involved in reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q9AJM0; Q9AJL9
involved in carbon dioxide fixation via a reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q9AJM0; Q9AJL9
activity of the recombinant enzyme with 2-oxobutyrate, 2-oxoisocaproate, 2-oxoisovalerate, oxalacetate, oxomalonate, pyruvate or phosphoenolpyruvate is less than 5% of the activity with 2-oxoglutarate
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin + H+
-
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin + H+
Helicobacter pylori DSM 1
-
-
-
-
?
2-oxoglutarate + CoA + oxidized methyl viologen
succinyl-CoA + CO2 + reduced methyl viologen
-
-
-
-
?
2-oxoglutarate + CoA + oxidized methyl viologen
succinyl-CoA + CO2 + reduced methyl viologen
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-
-
?
2-oxoglutarate + CoA + oxidized methyl viologen
succinyl-CoA + CO2 + reduced methyl viologen
-
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
A0L8G4; A0L8G5
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
A0L8G4; A0L8G5
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
may be involved in glutamate synthesis
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
of several ferredoxins tested, only those from the photosynthetic bacteria Chlorobium thiosulfatophilum, Chromatium, and Rhodospirillum rubrum are effective. The ferredoxins of Clostridium pasteurianum and spinach are poor substitutes for those of photosynthetic bacteria
-
-
r
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
may be involved in glutamate synthesis
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
O87870; Q8RJQ9
it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
-
-
r
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
CO2 exchange reaction and CO2 fixation, reductive TCA-cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
key enzyme in reductive tricarboxylic acid cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
CO2 exchange reaction and CO2 fixation, reductive TCA-cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
-
-
-
r
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
key enzyme in reductive tricarboxylic acid cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
enzyme plays a role in synthesis, not in energy conservation
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
enzyme plays a role in synthesis, not in energy conservation
-
-
?
succinyl-CoA + CO2 + reduced ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
-
-
-
-
r
succinyl-CoA + CO2 + reduced ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
-
involved in reductive TCA-cycle
-
-
r
succinyl-CoA + CO2 + reduced ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-
-
r
succinyl-CoA + CO2 + reduced ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
involved in reductive TCA-cycle
-
-
r
additional information
?
-
A0L8G4; A0L8G5
enzyme is both CO2-fixing and 2-oxoglutarate oxidizing. Negligible activity with other 2-oxoacids, including glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, 3-methyl-2-oxovalerate, phenylpyruvate and oxaloacetate and 2-oxoadipate
-
-
-
additional information
?
-
A0L8G4; A0L8G5
enzyme is both CO2-fixing and 2-oxoglutarate oxidizing. Negligible activity with other 2-oxoacids, including glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, 3-methyl-2-oxovalerate, phenylpyruvate and oxaloacetate and 2-oxoadipate
-
-
-
additional information
?
-
-
enzyme is both CO2-fixing and 2-oxoglutarate oxidizing. Negligible activity with other 2-oxoacids, including glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoisovalerate, 3-methyl-2-oxovalerate, phenylpyruvate and oxaloacetate and 2-oxoadipate
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Q9AJM0; Q9AJL9
involved in carbon dioxide fixation via a reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
involved in reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
involved in reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
one of the key enzymes of the reductive TCA cycle. The physiological function is the reductive carboxylation of succinyl-CoA to produce 2-oxoglutarate, growth under aerobic conditions. Under anaerobic conditions only the Kor enzyme is expressed. The enzyme For supports the optimal growth of the organism in the presence of oxygen
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q9AJM0; Q9AJL9
involved in carbon dioxide fixation via a reductive TCA cycle
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin + H+
-
-
-
-
?
2-oxoglutarate + CoA + oxidized ferredoxin
succinyl-CoA + CO2 + reduced ferredoxin + H+
Helicobacter pylori DSM 1
-
-
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
may be involved in glutamate synthesis
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
-
may be involved in glutamate synthesis
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + 2 oxidized ferredoxin
O87870; Q8RJQ9
it is proposed that the enzyme couples the complete acetyl-CoA oxidation to aromatic ring reduction by the use of the low-potential electron shuttle ferredoxin
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
CO2 exchange reaction and CO2 fixation, reductive TCA-cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
key enzyme in reductive tricarboxylic acid cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
CO2 exchange reaction and CO2 fixation, reductive TCA-cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
key enzyme in reductive tricarboxylic acid cycle
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
enzyme plays a role in synthesis, not in energy conservation
-
-
?
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
2-oxoglutarate + CoA + oxidized ferredoxin
-
enzyme plays a role in synthesis, not in energy conservation
-
-
?
succinyl-CoA + CO2 + reduced ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
-
involved in reductive TCA-cycle
-
-
r
succinyl-CoA + CO2 + reduced ferredoxin
2-oxoglutarate + CoA + oxidized ferredoxin
Hydrogenobacter thermophilus TK-6 / IAM 12695
-
involved in reductive TCA-cycle
-
-
r
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
[4Fe-4S]-center
A0L8G4; A0L8G5
protein contains 3.6 mol of iron and 3.4 mol of sulfur per mol of alphabeta heterodimer
additional information
O87870; Q8RJQ9
comparison of cofactor binding motifs of 2-ketoacid:acceptor oxidoreductases
-
additional information
-
comparison of cofactor binding motifs of 2-ketoacid:acceptor oxidoreductases
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Iron-sulfur cluster
additional information
-
no effect of Mg2+
Iron-sulfur cluster
O87870; Q8RJQ9
two [4Fe-4S] clusters per ferredoxin, 8.3 mol iron per mol of enzyme, 7.2 mol acid-labile sulfide per mol of enzyme
Iron-sulfur cluster
-
contains at least four [4Fe-4S] clusters per mol of holoenzyme
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no inhibition: CO, no inhibition at 5 mM: sodium benzoate, NaF, sodium hypophosphite, NaN3, KCN, 2-oxohexanoate, pyruvate, 2-oxobutyrate
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
thiamine diphosphate
-
0.9 mol per mol of enzyme, stimulates but is not essential, Km: 0.009 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.64
2-oxoglutarate
A0L8G4; A0L8G5
mutant E45Q, pH 7.5, temperature not specified in the publication
0.97
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
1.4
2-oxoglutarate
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
pH 7.8, 70°C, recombinant Kor
1.8
2-oxoglutarate
A0L8G4; A0L8G5
mutant Y436F, pH 7.5, temperature not specified in the publication
2.6
2-oxoglutarate
-
pH and temperature not specified in the publication
2.9
2-oxoglutarate
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
pH 7.8, 70°C, recombinant For
4.4
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 8.5, temperature not specified in the publication
4.9
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 7.5, temperature not specified in the publication
0.026
CoA
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
pH 7.8, 70°C, recombinant For
0.08
CoA
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
pH 7.8, 70°C, recombinant Kor
6.8
CoA
A0L8G4; A0L8G5
wild-type, pH 8.5, temperature not specified in the publication
10
CoA
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
0.008
oxidized ferredoxin
-
pH 8.4, 80°C, ferredoxin from Pyrococcus furiosus
1.8
oxidized ferredoxin
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
0.00055
reduced ferredoxin
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
isozyme Kor, in 10 mM MOPS, pH 6.8, at 70°C
0.0025
reduced ferredoxin
Q93RA3; Q93RA2, Q9AJM0; Q9AJL9
isozyme For, in 10 mM MOPS, pH 6.8, at 70°C
1.3
reduced ferredoxin
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
0.25
succinyl-CoA
-
pH and temperature not specified in the publication
32
succinyl-CoA
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
12.3
oxidized ferredoxin
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
0.51
reduced ferredoxin
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
0.45
succinyl-CoA
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
6.8
2-oxoglutarate
A0L8G4; A0L8G5
mutant E45Q, pH 7.5, temperature not specified in the publication
10.8
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
17.8
2-oxoglutarate
A0L8G4; A0L8G5
mutant Y436F, pH 7.5, temperature not specified in the publication
22.2
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 7.5, temperature not specified in the publication
30.3
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 8.5, temperature not specified in the publication
11.2
CoA
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
24.7
CoA
A0L8G4; A0L8G5
wild-type, pH 8.5, temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6800
oxidized ferredoxin
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
390
reduced ferredoxin
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
14
succinyl-CoA
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
4.5
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 7.5, temperature not specified in the publication
6.9
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 8.5, temperature not specified in the publication
9.9
2-oxoglutarate
A0L8G4; A0L8G5
mutant Y436F, pH 7.5, temperature not specified in the publication
11
2-oxoglutarate
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
11
2-oxoglutarate
A0L8G4; A0L8G5
mutant E45Q, pH 7.5, temperature not specified in the publication
1100
CoA
A0L8G4; A0L8G5
wild-type, pH 7.0, temperature not specified in the publication
3600
CoA
A0L8G4; A0L8G5
wild-type, pH 8.5, temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.25
-
pH and temperature not specified in the publication
3.34
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
70°C, pH not specified in the publication, recombinant enzyme, oxidative decarboxylation of 2-oxoglutarate
35.2
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
pH 7.8, 70°C, recombinant Kor
additional information
additional information
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0
the specific activity of For is approximately one-tenth of that of Kor
additional information
Q9AJM0; Q9AJL9
the specific activity of For is approximately one-tenth of that of Kor
additional information
-
the specific activity of For is approximately one-tenth of that of Kor
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 7.8
-
optimum with a sharp drop on the acidic side
7.7
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
recombinant Kor
8.7
D3DIA1; D3DIA2; D3DIA3; D3DIA0; Q93RA0, Q9AJM0; Q9AJL9
recombinant For, at 70°C, optimum varies between buffers