BRENDA - Enzyme Database show
show all sequences of 1.2.1.28

Benzoic acid biosynthesis in cell cultures of Hypericum androsaemum

Abd El-Mawla, A.M.A.; Beerhues, L.; Planta 214, 727-733 (2002)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NAD+ + H2O
Hypericum androsaemum
-
benzoate + NADH + H+
-
Hypericum androsaemum
?
benzaldehyde + NAD+ + H2O
Hypericum androsaemum
last step in the biosynthesis of benzoic acid from cinnamic acid, a precursor of xanthones
benzoate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hypericum androsaemum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
5-days-old, without or after treatment with methyl jasmonate for 6 h
Hypericum androsaemum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxybenzaldehyde + NAD+ + H2O
16% of activity with benzaldehyde
390243
Hypericum androsaemum
2-hydroxybenzoate + NADH + H+
-
390243
Hypericum androsaemum
?
2-hydroxybenzaldehyde + NAD+ + H2O
16% of the activity with benzaldehyde
390243
Hypericum androsaemum
2-hydroxybenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
38% of activity with benzaldehyde
390243
Hypericum androsaemum
3-hydroxybenzoate + NADH + H+
-
390243
Hypericum androsaemum
?
3-hydroxybenzaldehyde + NAD+ + H2O
38% of the activity with benzaldehyde
390243
Hypericum androsaemum
3-hydroxybenzoate + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
24% of activity with benzaldehyde
390243
Hypericum androsaemum
4-hydroxybenzoate + NADH + H+
-
390243
Hypericum androsaemum
?
4-hydroxybenzaldehyde + NAD+ + H2O
24% of the activity with benzaldehyde
390243
Hypericum androsaemum
4-hydroxybenzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
390243
Hypericum androsaemum
benzoate + NADH + H+
-
390243
Hypericum androsaemum
?
benzaldehyde + NAD+ + H2O
best substrate
390243
Hypericum androsaemum
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
last step in the biosynthesis of benzoic acid from cinnamic acid, a precursor of xanthones
390243
Hypericum androsaemum
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NADP+ + H2O
best substrate
390243
Hypericum androsaemum
benzoate + NADPH
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Hypericum androsaemum
35
-
-
Hypericum androsaemum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hypericum androsaemum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
; preferred cofactor
Hypericum androsaemum
NADP+
50% of the activity with NAD+
Hypericum androsaemum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
; preferred cofactor
Hypericum androsaemum
NADP+
50% of the activity with NAD+
Hypericum androsaemum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NAD+ + H2O
Hypericum androsaemum
-
benzoate + NADH + H+
-
Hypericum androsaemum
?
benzaldehyde + NAD+ + H2O
Hypericum androsaemum
last step in the biosynthesis of benzoic acid from cinnamic acid, a precursor of xanthones
benzoate + NADH + H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
5-days-old, without or after treatment with methyl jasmonate for 6 h
Hypericum androsaemum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxybenzaldehyde + NAD+ + H2O
16% of activity with benzaldehyde
390243
Hypericum androsaemum
2-hydroxybenzoate + NADH + H+
-
390243
Hypericum androsaemum
?
2-hydroxybenzaldehyde + NAD+ + H2O
16% of the activity with benzaldehyde
390243
Hypericum androsaemum
2-hydroxybenzoate + NADH + H+
-
-
-
?
3-hydroxybenzaldehyde + NAD+ + H2O
38% of activity with benzaldehyde
390243
Hypericum androsaemum
3-hydroxybenzoate + NADH + H+
-
390243
Hypericum androsaemum
?
3-hydroxybenzaldehyde + NAD+ + H2O
38% of the activity with benzaldehyde
390243
Hypericum androsaemum
3-hydroxybenzoate + NADH + H+
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
24% of activity with benzaldehyde
390243
Hypericum androsaemum
4-hydroxybenzoate + NADH + H+
-
390243
Hypericum androsaemum
?
4-hydroxybenzaldehyde + NAD+ + H2O
24% of the activity with benzaldehyde
390243
Hypericum androsaemum
4-hydroxybenzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
-
390243
Hypericum androsaemum
benzoate + NADH + H+
-
390243
Hypericum androsaemum
?
benzaldehyde + NAD+ + H2O
best substrate
390243
Hypericum androsaemum
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
last step in the biosynthesis of benzoic acid from cinnamic acid, a precursor of xanthones
390243
Hypericum androsaemum
benzoate + NADH + H+
-
-
-
?
benzaldehyde + NADP+ + H2O
best substrate
390243
Hypericum androsaemum
benzoate + NADPH
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Hypericum androsaemum
35
-
-
Hypericum androsaemum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Hypericum androsaemum
Other publictions for EC 1.2.1.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724140
Shrivastava
Purification and characterizat ...
Pseudomonas putida, Pseudomonas putida CSV86
Arch. Microbiol.
193
553-563
2011
-
-
-
-
-
2
14
12
-
2
2
2
-
2
-
-
1
-
-
-
2
1
29
1
-
-
-
11
1
-
-
2
11
-
-
-
-
-
2
-
-
2
-
14
11
12
-
2
2
2
-
-
-
1
-
-
2
1
29
1
-
-
-
11
1
-
-
-
-
1
1
-
11
11
699798
Gaid
Benzaldehyde dehydrogenase fro ...
Sorbus aucuparia
J. Plant Physiol.
166
1343-1349
2009
1
-
-
-
-
-
2
2
-
-
-
1
-
1
-
-
1
-
-
-
2
-
1
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
2
-
2
-
-
-
1
-
-
-
1
-
-
2
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
700759
Long
Involvement of snapdragon benz ...
Antirrhinum majus
Plant J.
59
256-265
2009
-
-
1
-
-
-
1
3
1
-
2
1
-
3
-
-
1
-
-
1
1
-
11
1
1
-
-
3
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
3
1
-
2
1
-
-
-
1
-
1
1
-
11
1
1
-
-
3
1
1
-
-
-
-
-
-
-
-
695093
Goettsching
Productive degradation of the ...
Acinetobacter sp. AG1
Res. Microbiol.
158
251-257
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
-
2
-
1
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1
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-
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2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676831
van Sint Fiet
Selection of biocatalysts for ...
Pseudomonas putida
Proc. Natl. Acad. Sci. USA
103
1693-1698
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
-
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2
-
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1
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1
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
390243
Abd El-Mawla
Benzoic acid biosynthesis in c ...
Hypericum androsaemum
Planta
214
727-733
2002
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
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1
-
-
10
-
2
-
-
-
1
-
-
2
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
10
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
390242
Kim
-
DNA sequence of the phnN gene ...
Pseudomonas sp., Pseudomonas sp. DJ77
J. Microbiol.
37
224-228
1999
-
-
1
-
-
-
-
-
-
-
1
2
-
4
-
-
-
-
-
-
-
-
11
1
-
-
-
-
1
-
-
1
-
-
-
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-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
11
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
390241
Gillooly
Molecular characterization of ...
Acinetobacter calcoaceticus
Biochem. J.
330
1375-1381
1998
-
-
1
-
-
-
-
-
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1
1
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1
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1
1
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1
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1
1
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1
1
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1
1
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-
-
-
-
-
-
-
-
-
-
-
-
390225
Inoue
Overlapping substrate specific ...
Pseudomonas putida
J. Bacteriol.
177
1196-1201
1995
-
-
-
-
-
1
-
8
-
-
4
2
-
1
-
-
1
-
-
-
1
-
16
1
-
-
-
-
1
1
-
1
-
-
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-
1
-
-
1
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-
-
8
-
-
4
2
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-
1
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1
-
16
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
4814
Tsou
Mandelate pathway of Pseudomon ...
Pseudomonas putida
Biochemistry
29
9856-9862
1990
-
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1
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1
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1
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1
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1
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1
1
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1
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1
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-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
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-
9
-
2
2
2
-
2
-
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1
-
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1
-
10
2
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1
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-
4
-
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4
-
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9
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2
2
2
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1
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1
-
10
2
-
-
-
-
1
-
-
-
-
-
-
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-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
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-
1
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-
-
-
-
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1
-
1
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1
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1
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1
1
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1
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1
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-
389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
-
-
-
-
-
-
-
-
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1
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1
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4
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1
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1
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1
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4
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390228
Chalmers
Purification and characterizat ...
Acinetobacter calcoaceticus
Biochem. J.
263
913-919
1989
-
-
-
-
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5
3
-
1
2
1
-
1
-
-
1
-
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-
1
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21
1
-
-
-
-
1
-
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2
-
-
-
-
-
-
2
-
-
-
-
5
-
3
-
1
2
1
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1
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1
-
21
1
-
-
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-
1
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-
-
-
-
-
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-
-
288330
Shaw
Physical and functional mappin ...
Pseudomonas putida
J. Gen. Microbiol.
134
2463-2474
1988
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1
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1
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1
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1
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1
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1
1
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1
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1
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389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
-
-
-
-
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2
-
2
-
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2
1
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1
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1
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1
1
2
1
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1
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1
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1
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2
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2
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2
1
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1
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1
1
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
255
653-661
1988
-
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-
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-
10
16
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-
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1
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1
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17
-
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16
-
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1
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1
-
-
-
-
10
-
16
-
-
-
1
-
-
-
-
-
-
-
-
17
-
-
-
-
16
-
-
-
-
-
-
-
-
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-
390233
Chalmers
-
Quantitative immunoblotting in ...
Acinetobacter calcoaceticus
Biochem. Soc. Trans.
16
153-154
1988
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2
1
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1
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1
1
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1
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1
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2
1
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1
1
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288331
Keil
Gene organization of the first ...
Pseudomonas putida
J. Bacteriol.
169
764-770
1987
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1
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1
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1
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1
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1
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1
1
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1
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1
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390234
Van den Tweel
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Physical and functional mappin ...
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745-754
1987
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390237
MacKintosh
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Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
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1048-1049
1987
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2
5
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4
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4801
Van den Tweel
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Microbial metabolism of D- and ...
Pseudomonas putida
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169-174
1986
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288333
Bhat
Involvement of 4-hydroxymandel ...
Pseudomonas putida
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1108-1118
1976
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390239
Livingstone
Two benzaldehyde dehydrogenase ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus N.C.I.B. 8250
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1972
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Fewson
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Regulation of enzymes of the m ...
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1968
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