Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 0.5 mM, complete inhibition | Acinetobacter calcoaceticus | |
N-ethylmaleimide | 0.5 mM, complete inhibition | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | benzaldehyde dehydrogenase I | Acinetobacter calcoaceticus | |
K+ | or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ | Acinetobacter calcoaceticus | |
K+ | only dehydrogenase I of strain N.C.I.B. 8250 is activated by univalent cations | Acinetobacter calcoaceticus | |
Li+ | benzaldehyde dehydrogenase I | Acinetobacter calcoaceticus | |
Na+ | benzaldehyde dehydrogenase I | Acinetobacter calcoaceticus | |
NH4+ | benzaldehyde dehydrogenase I | Acinetobacter calcoaceticus | |
Rb+ | benzaldehyde dehydrogenase I | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
strain N.C.I.B. 8250, gel filtration | Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | Acinetobacter calcoaceticus | - |
benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | Acinetobacter calcoaceticus N.C.I.B. 8250 | - |
benzoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
N.C.I.B. 8250 | - |
Acinetobacter calcoaceticus N.C.I.B. 8250 | - |
N.C.I.B. 8250 | - |
Purification (Comment) | Organism |
---|---|
dehydrogenases I and II | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
benzaldehyde dehydrogenase II and class 1 mutant activity in cell extracts of wild-type grown without inducer | Acinetobacter calcoaceticus |
0.006 | - |
class 1 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol or 1 mM benzaldehyde | Acinetobacter calcoaceticus |
0.094 | - |
class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde | Acinetobacter calcoaceticus |
0.126 | - |
benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 1 mM benzaldehyde | Acinetobacter calcoaceticus |
0.324 | - |
benzaldehyde dehydrogenase II activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol | Acinetobacter calcoaceticus |
0.325 | - |
class 2 mutant enzyme activity in cell extracts of wild-type grown in the presence of 5 mM benzyl alcohol | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus N.C.I.B. 8250 | benzoate + NADH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-lives: benzaldehyde dehydrogenase I, 520 min, benzaldehyde dehydrogenase II, 30 min, pH 9.18, half-life for benzaldehyde dehydrogenase II increases to 113 min at pH 8.5 | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter calcoaceticus |