KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00038 | - |
3-methylbenzaldehyde | - |
Pseudomonas putida | |
0.00097 | - |
4-methylbenzaldehyde | - |
Pseudomonas putida | |
0.0012 | - |
3-methylbenzaldehyde | - |
Acinetobacter calcoaceticus | |
0.003 | - |
4-methylbenzaldehyde | - |
Pseudomonas putida | |
0.003 | - |
4-methylbenzaldehyde | - |
Acinetobacter calcoaceticus | |
0.026 | - |
2-Methylbenzaldehyde | - |
Pseudomonas putida | |
0.088 | - |
2-Methylbenzaldehyde | - |
Acinetobacter calcoaceticus | |
0.33 | - |
NAD+ | - |
Pseudomonas putida | |
0.46 | - |
benzaldehyde | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ | Pseudomonas putida | |
K+ | or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ | Acinetobacter calcoaceticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56300 | - |
4 * 56300, SDS-PAGE | Pseudomonas putida |
122000 | 126000 | gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NAD+ + H2O | Pseudomonas putida | - |
benzoate + NADH + H+ | - |
ir | |
benzaldehyde + NAD+ + H2O | Acinetobacter calcoaceticus | - |
benzoate + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.2 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 2-methylbenzoate + NADH | - |
? | |
2-methylbenzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | 2-methylbenzoate + NADH | - |
? | |
3-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-methylbenzoate + NADH | - |
? | |
3-methylbenzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | 3-methylbenzoate + NADH | - |
? | |
4-methylbenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-methylbenzoate + NADH | - |
? | |
4-methylbenzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | 4-methylbenzoate + NADH | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + H+ | - |
ir | |
benzaldehyde + NAD+ + H2O | - |
Acinetobacter calcoaceticus | benzoate + NADH + H+ | - |
ir | |
benzaldehyde + NAD+ + H2O | also active with NADP+, see also EC 1.2.1.7 | Pseudomonas putida | benzoate + NADH + H+ | - |
ir | |
benzaldehyde + NAD+ + H2O | also active with NADP+, see also EC 1.2.1.7 | Acinetobacter calcoaceticus | benzoate + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Acinetobacter calcoaceticus |
tetramer | 4 * 56300, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NAD+ | - |
Acinetobacter calcoaceticus | |
NADP+ | - |
Acinetobacter calcoaceticus | |
NADP+ | enzyme on Pseudomonas plasmid | Pseudomonas putida |