Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12800 | - |
x * 23800, small subunit NicA, + x * 12800, large subunit NicB | Pseudomonas putida |
23800 | - |
x * 23800, small subunit NicA, + x * 12800, large subunit NicB | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
KT2440 | - |
Pseudomonas putida KT 2240 | - |
KT2440 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 5-methylphenazinium methyl sulfate + H2O | 4% of the activity with nicotinate | Pseudomonas putida | ? | - |
? | |
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O | - |
Pseudomonas putida | 6-hydroxynicotinic acid + ? | - |
? | |
nipecotic acid + 5-methylphenazinium methyl sulfate + H2O | 8% of the activity with nicotinate | Pseudomonas putida | ? | - |
? | |
picolinic acid + 5-methylphenazinium methyl sulfate + H2O | 7% of the activity with nicotinate | Pseudomonas putida | ? | - |
? | |
pyrazine-2-carboxylic acid + 5-methylphenazinium methyl sulfate + H2O | 8% of the activity with nicotinate | Pseudomonas putida | ? | - |
? | |
pyridine-3-carboxyaldehyde + 5-methylphenazinium methyl sulfate + H2O | 5% of the activity with nicotinate | Pseudomonas putida | ? | - |
? | |
pyridine-3-sulfonic acid + 5-methylphenazinium methyl sulfate + H2O | 4% of the activity with nicotinate | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23800, small subunit NicA, + x * 12800, large subunit NicB | Pseudomonas putida |
More | small subunit NicA harbors the 41-C-X4-C-G-X-C-Xn-C-59 and 100-C-G-X-C-X31-C-X-C-137 conserved motifs likely involved in binding of the two [2Fe-2S] clusters | Pseudomonas putida |
More | the N-terminal region of large subunit NicB contains the active site and the molybdopterin cytosine dinucleotide binding sites in an arrangement MPT2-MPT1-MPT3. The C-terminal region of NicB, residues750-1187, contains three conserved cytochrome c hemebinding motifs 817-CAVCH-823, 963-CTACH-969, and 1087-CLGCH-1093 | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
NicA | - |
Pseudomonas putida |
NicB | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2Fe-2S-center | - |
Pseudomonas putida | |
cytochrome c | - |
Pseudomonas putida | |
molybdopterin cytosine dinucleotide | - |
Pseudomonas putida |