BRENDA - Enzyme Database
show all sequences of 1.14.99.63

Characterization of beta-carotene ketolases, CrtW, from marine bacteria by complementation analysis in Escherichia coli

Choi, S.; Nishida, Y.; Matsuda, S.; Adachi, K.; Kasai, H.; Peng, X.; Komemushi, S.; Miki, W.; Misawa, N.; Mar. Biotechnol. 7, 515-522 (2005)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX
Brevundimonas sp. SD212
expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX
Paracoccus sp. N81106
expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX
Paracoccus sp. PC1
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
Brevundimonas sp. SD212
the enzyme is involved in the astaxanthin biosynthetic pathway
astaxanthin + 2 acceptor + 3 H2O
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
Paracoccus sp. N81106
the enzyme is involved in the astaxanthin biosynthetic pathway
astaxanthin + 2 acceptor + 3 H2O
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
Paracoccus sp. PC1
the enzyme is involved in the astaxanthin biosynthetic pathway
astaxanthin + 2 acceptor + 3 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevundimonas sp. SD212
Q4W8B8
-
-
Paracoccus sp. N81106
P54972
-
-
Paracoccus sp. PC1
Q44261
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
-
748526
Paracoccus sp. N81106
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
-
748526
Paracoccus sp. PC1
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in the astaxanthin biosynthetic pathway
748526
Brevundimonas sp. SD212
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in the astaxanthin biosynthetic pathway
748526
Paracoccus sp. N81106
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in the astaxanthin biosynthetic pathway
748526
Paracoccus sp. PC1
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
efficient conversion by CrtW of Brevundimonas sp. SD212 from adonixanthin to astaxanthin
748526
Brevundimonas sp. SD212
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX
Brevundimonas sp. SD212
expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX
Paracoccus sp. N81106
expression in Escherichia coli synthesizing zeaxanthin due to the presence of plasmid pACCAR25DELTAcrtX
Paracoccus sp. PC1
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
Brevundimonas sp. SD212
the enzyme is involved in the astaxanthin biosynthetic pathway
astaxanthin + 2 acceptor + 3 H2O
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
Paracoccus sp. N81106
the enzyme is involved in the astaxanthin biosynthetic pathway
astaxanthin + 2 acceptor + 3 H2O
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
Paracoccus sp. PC1
the enzyme is involved in the astaxanthin biosynthetic pathway
astaxanthin + 2 acceptor + 3 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adonixanthin + 2 reduced acceptor + 2 O2
-
748526
Paracoccus sp. N81106
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
-
748526
Paracoccus sp. PC1
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in the astaxanthin biosynthetic pathway
748526
Brevundimonas sp. SD212
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in the astaxanthin biosynthetic pathway
748526
Paracoccus sp. N81106
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
the enzyme is involved in the astaxanthin biosynthetic pathway
748526
Paracoccus sp. PC1
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
adonixanthin + 2 reduced acceptor + 2 O2
efficient conversion by CrtW of Brevundimonas sp. SD212 from adonixanthin to astaxanthin
748526
Brevundimonas sp. SD212
astaxanthin + 2 acceptor + 3 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the astaxanthin biosynthetic pathway
Brevundimonas sp. SD212
metabolism
the enzyme is involved in the astaxanthin biosynthetic pathway
Paracoccus sp. N81106
metabolism
the enzyme is involved in the astaxanthin biosynthetic pathway
Paracoccus sp. PC1
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the astaxanthin biosynthetic pathway
Brevundimonas sp. SD212
metabolism
the enzyme is involved in the astaxanthin biosynthetic pathway
Paracoccus sp. N81106
metabolism
the enzyme is involved in the astaxanthin biosynthetic pathway
Paracoccus sp. PC1
Other publictions for EC 1.14.99.63
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742396
Zhou
Alleviation of metabolic bott ...
Haematococcus lacustris
Enzyme Microb. Technol.
100
28-36
2017
-
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1
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7
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2
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1
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2
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1
1
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742573
Wang
-
Engineering of beta-carotene ...
Brevundimonas vesicularis, Brevundimonas vesicularis DC263
Front. Chem. Sci. Engin.
11
89-99
2017
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1
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3
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743205
Lu
Metabolic engineering of Esch ...
Brevundimonas sp. SD212, Paracoccus sp. N81106, Paracoccus sp. PC1, Sphingomonas sp. DC18
Mar. Drugs
15
E296
2017
-
1
4
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8
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4
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16
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4
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8
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16
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4
4
-
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743873
Bai
Reconstruction of the astaxan ...
Chlamydomonas reinhardtii
Transgenic Res.
26
13-23
2017
-
1
1
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-
-
-
-
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1
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749390
Kildegaard
Engineering of Yarrowia lipol ...
Paracoccus sp. N81106
Synth. Syst. Biotechnol.
2
287-294
2017
-
1
-
-
-
-
-
-
-
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1
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743898
Sandmann
Control of light-dependent ke ...
Nostoc sp. PCC 7120
Z. Naturforsch. C
71
303-311
2016
-
-
-
-
-
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1
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1
1
1
1
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-
742948
Kathiresan
Regulation of astaxanthin and ...
Haematococcus lacustris, Haematococcus lacustris SAG 34-1a
J. Biotechnol.
196-197
33-41
2015
-
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1
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2
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1
1
-
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-
720799
Schöpf
Multiple ketolases involved in ...
Nostoc punctiforme
Planta
237
1279-1285
2013
-
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1
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4
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2
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2
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748319
Zhong
Functional characterization o ...
Haematococcus lacustris
J. Exp. Bot.
62
3659-3669
2011
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1
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1
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1
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1
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716454
Kim
Over-expression of Arabidopsis ...
Haematococcus lacustris
Phytochemistry
71
168-178
2010
-
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1
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1
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1
1
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746819
Ukibe
Metabolic engineering of Sacc ...
Xanthophyllomyces dendrorhous, Xanthophyllomyces dendrorhous ATCC 24202
Appl. Environ. Microbiol.
75
7205-7211
2009
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-
1
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2
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2
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4
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1
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1
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4
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747340
Scaife
Characterization of cyanobact ...
Nostoc punctiforme, Synechocystis sp. PCC 6803
Biotechnol. Bioeng.
103
944-955
2009
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-
2
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2
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1
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748597
Martin
Conversion of beta-carotene i ...
Xanthophyllomyces dendrorhous, Xanthophyllomyces dendrorhous ATCC 96815
Microb. Cell Fact.
7
3
2008
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4
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2
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8
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8
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1
1
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749393
Shindo
-
4-Ketoantheraxanthin, a novel ...
Brevundimonas sp. SD212
Tetrahedron Lett.
49
3294-3296
2008
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1
1
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1
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1
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1
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1
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746871
Choi
Characterization of two beta- ...
Brevundimonas sp. SD212, Rhodococcus erythropolis, Rhodococcus erythropolis PR4, Synechosystis sp. PCC6803
Appl. Microbiol. Biotechnol.
75
1335-1341
2007
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3
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4
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12
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3
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12
-
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-
-
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746817
Ye
Mutational and functional ana ...
Paracoccus sp. N81106
Appl. Environ. Microbiol.
72
5829-5837
2006
-
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1
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20
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4
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20
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2
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747895
Tao
A carotenoid synthesis gene c ...
Brevundimonas vesicularis, Brevundimonas vesicularis DC2630
Gene
379
101-108
2006
-
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1
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2
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748562
Tao
Engineering a beta-carotene k ...
Sphingomonas sp. DC18
Metab. Eng.
8
523-531
2006
-
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1
-
7
-
-
-
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2
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1
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2
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1
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7
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2
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2
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748658
Ojima
Cloning of the astaxanthin sy ...
Xanthophyllomyces dendrorhous, Xanthophyllomyces dendrorhous ATCC 96594
Mol. Genet. Genomics
275
148-158
2006
-
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5
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4
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1
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1
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4
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748526
Choi
-
Characterization of beta-caro ...
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748657
Tao
Novel beta-carotene ketolases ...
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