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Literature summary for 1.14.20.6 extracted from

  • Toh, H.; Wang, S.; Chang, S.; Chu, F.
    Molecular cloning and characterization of flavonol synthase in Acacia confusa (2013), Tree Genet. Genomes, 9, 85-92.
No PubMed abstract available
Search for more literature for 1.14.20.6

Cloned(Commentary)

Cloned (Comment) Organism
gene AcFLS, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged FLS protein in Escherichia coli strain BL21-CodonPlus-RIL Acacia confusa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, the enzyme contains a ferrous iron (II) binding residue motif, i.e. the HxDxnH motif consisting of His217, Asp219, and His273 Acacia confusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, about, sequence calculation Acacia confusa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 Acacia confusa
-
 quercetin + succinate + CO2 + H2O
-
 ?
dihydromyricetin + 2-oxoglutarate + O2 Acacia confusa
-
 myricetin + succinate + CO2 + H2O
-
 ?
naringenin + 2-oxoglutarate + O2 Acacia confusa
-
 kaempferol + succinate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acacia confusa H6V8U4 gene AcFLS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged FLS protein from Escherichia coli strain BL21-CodonPlus-RIL by nickel affinity chromatography Acacia confusa

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Acacia confusa
-
leaf
-
 Acacia confusa
-
seedling
-
 Acacia confusa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
 purified recombinant His-tagged enzyme, substrate dihydroquercetin, pH 6.8, 25°C Acacia confusa
0.154
-
 purified recombinant His-tagged enzyme, substrate dihydromyricetin, pH 6.8, 25°C Acacia confusa
0.163
-
 purified recombinant His-tagged enzyme, substrate naringenin, pH 6.8, 25°C Acacia confusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R/3R)-dihydroquercetin + 2-oxoglutarate + O2
-
 Acacia confusa quercetin + succinate + CO2 + H2O
-
 ?
dihydromyricetin + 2-oxoglutarate + O2
-
 Acacia confusa myricetin + succinate + CO2 + H2O
-
 ?
additional information substrates are bound with similar affinity Acacia confusa ?
-
 ?
naringenin + 2-oxoglutarate + O2
-
 Acacia confusa kaempferol + succinate + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, about, sequence calculation Acacia confusa
More FLS protein structure modeling, overview Acacia confusa

Synonyms

Synonyms Comment Organism
FLS
-
 Acacia confusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Acacia confusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Acacia confusa

pI Value

Organism Comment pI Value Maximum pI Value
Acacia confusa sequence calculation
-
 5.7

Expression

Organism Comment Expression
Acacia confusa wounding by cutting leaves induces maximum AcFLS mRNA accumulation 6-12 h after treatment up

General Information

General Information Comment Organism
evolution the enzyme belongs to the the 2-oxoglutarate-Fe(II) dioxygenase superfamily Acacia confusa
additional information FLS protein structure modeling, overview. The structures of two major flavonols, i.e. melanoxetin or transilitin, found in Acacia confusa heartwood are different from common flavonoids Acacia confusa