Any feedback?
Literature summary for 1.14.20.6 extracted from
- Functional expression and mutational analysis of flavonol synthase from Citrus unshiu (2002), Eur. J. Biochem., 269, 4134-4142.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G261A | mutant enzyme with 10% of wild-type activity | Citrus unshiu |
| G261P | no immunoreactive FLS polypeptide detected | Citrus unshiu |
| G68A | mutant enzyme with 6% of wild-type activity | Citrus unshiu |
| G68P | no immunoreactive FLS polypeptide detected | Citrus unshiu |
| P207G | no effect on activity | Citrus unshiu |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
2-oxoglutarate | - |
Citrus unshiu |  |
| 0.045 | - |
dihydrokaempferol | - |
Citrus unshiu | |
| 0.272 | - |
dihydroquercetin | - |
Citrus unshiu | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, Km-value: 0.011 mM | Citrus unshiu | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37899 | - |
x * 37899, MALDI-TOF mass spectrometry | Citrus unshiu |
| 38000 | - |
x * 38000, SDS-PAGE | Citrus unshiu |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus unshiu | Q9ZWQ9 | Satsuma orange | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrokaempferol + 2-oxoglutarate + O2 | - |
Citrus unshiu | kaempferol + succinate + CO2 + H2O | - |
? | |
| dihydrokaempferol + 2-oxoglutarate + O2 | - |
Citrus unshiu | kaempferol + succinate + CO2 + H2O | - |
r | |
| dihydroquercetin + 2-oxoglutarate + O2 | - |
Citrus unshiu | quercetin + succinate + CO2 + H2O | - |
? | |
| dihydroquercetin + 2-oxoglutarate + O2 | - |
Citrus unshiu | quercetin + succinate + CO2 + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Citrus unshiu |
| ? | x * 37899, MALDI-TOF mass spectrometry | Citrus unshiu |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
reaction with dihydroquercetin | Citrus unshiu |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
reaction with dihydroquercetin | Citrus unshiu |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
