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Literature summary for 1.14.19.13 extracted from
- Targeted mutation of DELTA12 and DELTA15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil (2014), Plant Biotechnol. J., 12, 613-623 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FAD3A, quantitative RT-PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae, CSFAD3A produces 18:2DELTA9,15 from endogenous 18:1DELTA9 when expressed in Saccharomyces cerevisiae | Cannabis sativa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Cannabis sativa | 5783 | - |
| membrane | membrane-bound | Cannabis sativa | 16020 | - |
| microsome | - |
Cannabis sativa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Cannabis sativa | the FAD3 enzyme is responsible for the DELTA15 desaturation of linoleic acid (18:2DELTA9,12) to alpha-linolenoic acid (18:3DELTA9,12,15) and of gamma-linolenic acid (18:3DELTA6,9,12) to stearidonic acid (18:4DELTA6,9,12,15). CSFAD3A acts as a DELTA15 desaturase in vivo as well as in a heterologous host | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cannabis sativa | A0A088MER7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Cannabis sativa | - |
| leaf | - |
Cannabis sativa | - |
| additional information | expression of CSFAD3A in both leaves and embryos, it is induced during seed development peaking at the FND stage where it is about 14 times higher than levels in young leaves, quantitative RT-PCR expression analysis | Cannabis sativa | - |
| seed | developing | Cannabis sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the FAD3 enzyme is responsible for the DELTA15 desaturation of linoleic acid (18:2DELTA9,12) to alpha-linolenoic acid (18:3DELTA9,12,15) and of gamma-linolenic acid (18:3DELTA6,9,12) to stearidonic acid (18:4DELTA6,9,12,15). CSFAD3A acts as a DELTA15 desaturase in vivo as well as in a heterologous host | Cannabis sativa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CSFAD3A | - |
Cannabis sativa |
| DELTA 15 desaturase | - |
Cannabis sativa |
| DELTA15 desaturase | - |
Cannabis sativa |
| FAD3A | - |
Cannabis sativa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | all members of the CSFAD3 subclass contain eight exons and seven introns within the gene. Evolutionary relationship of Cannabis sativa putative desaturases, overview | Cannabis sativa |
| additional information | hemp seeds contain three DELTA15 desaturases, FAD3A is the major DELAT15 desaturase in hemp seed | Cannabis sativa |
| physiological function | the FAD3 enzyme is responsible for the DELTA15 desaturation of linoleic acid (18:2DELTA9,12) to alpha-linolenoic acid (18:3DELTA9,12,15) | Cannabis sativa |
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