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Literature summary for 1.14.19.13 extracted from
- Molecular cloning and characterization of the genes encoding a microsomal oleate DELTA12 desaturase (CsFAD2) and linoleate DELTA15 desaturase (CsFAD3) from Camelina sativa (2016), Ind. Crops Prod., 89, 405-415 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CsFAD3, variant FAD3-1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview | Camelina sativa |
| gene CsFAD3, variant FAD3-2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview | Camelina sativa |
| gene CsFAD3, variant FAD3-3, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, subcloning in Escherichia coli strain XL1-Blue, and functional recombinant expression in Saccharomyces cerevisiae strain W303-1A. The conversion driven by recombinant CsFAD3 is enhanced 5fold at 22°C compared to 30°C, although it is not able to desaturate 16:2DELTA9,12. Fatty acid composition of Saccharomyces cerevisiae cells overexpressing CsFAD3 microsomal desaturase and grown at two different temperatures, overview | Camelina sativa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Camelina sativa | 5783 | - |
| membrane | integral membrane enzyme | Camelina sativa | 16020 | - |
| microsome | - |
Camelina sativa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | two Fe2+ ions bound in the His box | Camelina sativa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelina sativa | A0A059V6T7 | FAD3-2 | - |
| Camelina sativa | A0A059V8B3 | FAD3-3 | - |
| Camelina sativa | A0A059V8R1 | FAD3-1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Camelina sativa | - |
| hypocotyl | - |
Camelina sativa | - |
| leaf | - |
Camelina sativa | - |
| root | - |
Camelina sativa | - |
| seed | developing, high expression level, particularly from 6 to 12 days after flowering and from 18 to 24 days after flowering, corresponding with the phases of seed expansion and oil accumulation respectively | Camelina sativa | - |
| seed | developing, high expression level, particularly from 6 to 12 days of flowering and from 18 to 24 days of flowering, corresponding with the phases of seed expansion and oil accumulation respectively | Camelina sativa | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44300, about, sequence calculation | Camelina sativa |
| ? | x * 44400, about, sequence calculation | Camelina sativa |
| ? | x * 44500, about, sequence calculation | Camelina sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CsFAD3 | - |
Camelina sativa |
| FAD3 | - |
Camelina sativa |
| FAD3-1 | - |
Camelina sativa |
| FAD3-2 | - |
Camelina sativa |
| FAD3-3 | - |
Camelina sativa |
| linoleate DELTA15desaturase | - |
Camelina sativa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Camelina sativa | sequence calculation | - |
8.4 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | three different copies of the genes FAD2 and FAD3 are identified, which contain three histidine rich motifs (HXCGHX, HRXHH andHVXHH) and six highly conserved transmembrane domains. Comparing their sequences, the CsFAD2copies accommodate four conservative changes (E36D, R48H, V97A, and A177P) and two semi-conservative ones (V63I and L249M), whereas only one semi-conservative change (A327S) is detected in CsFAD3 but with two extra amino acids (H147 and G148) | Camelina sativa |
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