BRENDA - Enzyme Database show
show all sequences of 1.14.18.5

Characterization of bifunctional sphingolipid DELTA4-desaturases/C4-hydroxylases of trypanosomatids by liquid chromatography-electrospray tandem mass spectrometry

Vacchina, P.; Tripodi, K.E.; Escalante, A.M.; Uttaro, A.D.; Mol. Biochem. Parasitol. 184, 29-38 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae DELTAsur2 mutant
Leishmania major
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leishmania major
G3FCJ2
Leishmania major has four genes occurring in tandem arrays, with at least three of them encoding active bifunctional enzymes
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Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is a bifunctional sphingolipid DELTA4-desaturase/C4-hydroxylase, with the desaturase activity being one order of magnitude higher than the hydroxylase activity
734653
Leishmania major
?
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-
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Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae DELTAsur2 mutant
Leishmania major
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme is a bifunctional sphingolipid DELTA4-desaturase/C4-hydroxylase, with the desaturase activity being one order of magnitude higher than the hydroxylase activity
734653
Leishmania major
?
-
-
-
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Other publictions for EC 1.14.18.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734653
Vacchina
Characterization of bifunction ...
Leishmania major
Mol. Biochem. Parasitol.
184
29-38
2012
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720322
Moreno-Perez
Sphingolipid base modifying en ...
Helianthus annuus
J. Plant Physiol.
168
831-839
2011
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719485
Mizutani
Identification of the human sp ...
Homo sapiens
FEBS Lett.
563
93-97
2004
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5
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719486
Idkowiak-Baldys
Sphingolipid C4 hydroxylation ...
Saccharomyces cerevisiae
FEBS Lett.
569
272-276
2004
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1
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721116
Bae
Cloning and functional charact ...
Wickerhamomyces ciferrii
Yeast
21
437-443
2004
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1
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3
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1
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733207
Wright
Synthesis of 4-hydroxysphingan ...
Zea mays
Arch. Biochem. Biophys.
415
184-192
2003
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3
1
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733375
Idkowiak-Baldys
Structure-function studies of ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1618
17-24
2003
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19
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19
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1
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734141
Ternes
Identification and characteriz ...
Mus musculus
J. Biol. Chem.
277
25512-25518
2002
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719481
Sperling
Functional characterization of ...
Arabidopsis thaliana
FEBS Lett.
494
90-94
2001
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1
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4
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719795
Grilley
Syringomycin action gene SYR2 ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae W303C
J. Biol. Chem.
273
11062-11068
1998
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4
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2
2
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719792
Haak
Hydroxylation of Saccharomyces ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
29704-29710
1997
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