BRENDA - Enzyme Database
show all sequences of 1.14.18.5

Syringomycin action gene SYR2 is essential for sphingolipid 4-hydroxylation in Saccharomyces cerevisiae

Grilley, M.; Stock, S.; Dickson, R.; Lester, R.; Takemoto, J.; J. Biol. Chem. 273, 11062-11068 (1998)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane protein
Saccharomyces cerevisiae
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
a diiron binding enzyme
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae W303C
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dihydroceramide + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae
?
-
-
-
?
dihydroceramide + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae W303C
?
-
-
-
?
DL-erythro-dihydrosphingosine + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae
phytosphingosine + acceptor + H2O
-
-
-
?
DL-erythro-dihydrosphingosine + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae W303C
phytosphingosine + acceptor + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
DHS hydroxylase
-
Saccharomyces cerevisiae
dihydroceramide hydrolase
-
Saccharomyces cerevisiae
SUR2
-
Saccharomyces cerevisiae
SYR2
-
Saccharomyces cerevisiae
Syr2p
-
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
integral membrane protein
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
a diiron binding enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dihydroceramide + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae
?
-
-
-
?
dihydroceramide + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae W303C
?
-
-
-
?
DL-erythro-dihydrosphingosine + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae
phytosphingosine + acceptor + H2O
-
-
-
?
DL-erythro-dihydrosphingosine + reduced acceptor + H+ + O2
-
719795
Saccharomyces cerevisiae W303C
phytosphingosine + acceptor + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
yeast strains deficient in Syr2 lack the sphingoid long chain base phytosphingosine
Saccharomyces cerevisiae
physiological function
SYR2 is necessary for growth inhibition by the cyclic lipodepsipeptide syringomycin E. The SYR2 gene is required for the 4-hydroxylation reaction of sphingolipid long chain bases and is not essential for growth. SYR2 does not play a role in sterol biosynthesis
Saccharomyces cerevisiae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
yeast strains deficient in Syr2 lack the sphingoid long chain base phytosphingosine
Saccharomyces cerevisiae
physiological function
SYR2 is necessary for growth inhibition by the cyclic lipodepsipeptide syringomycin E. The SYR2 gene is required for the 4-hydroxylation reaction of sphingolipid long chain bases and is not essential for growth. SYR2 does not play a role in sterol biosynthesis
Saccharomyces cerevisiae
Other publictions for EC 1.14.18.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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720322
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168
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1
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1
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1
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719486
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FEBS Lett.
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1
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721116
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1
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Synthesis of 4-hydroxysphingan ...
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415
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2003
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3
1
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7
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1
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733375
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Biochim. Biophys. Acta
1618
17-24
2003
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19
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2
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19
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1
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1
1
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719481
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FEBS Lett.
494
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2001
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1
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719795
Grilley
Syringomycin action gene SYR2 ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae W303C
J. Biol. Chem.
273
11062-11068
1998
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4
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2
2
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719792
Haak
Hydroxylation of Saccharomyces ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
29704-29710
1997
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