BRENDA - Enzyme Database show
show all sequences of 1.14.13.226

Novel acetone metabolism in a propane-utilizing bacterium, Gordonia sp. strain TY-5

Kotani, T.; Yurimoto, H.; Kato, N.; Sakai, Y.; J. Bacteriol. 189, 886-893 (2007)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
4 * 63000, SDS-PAGE
Gordonia sp. TY-5
230000
-
gel filtration
Gordonia sp. TY-5
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetone + NADPH + H+ + O2
Gordonia sp. TY-5
-
methyl acetate + NADP+ + H2O
enzyme catalyzes stoichiometric conversion of acetone to methyl acetate with consumption of NADPH
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gordonia sp. TY-5
A1IHE6
-
-
Purification (Commentary)
Commentary
Organism
-
Gordonia sp. TY-5
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetone + NADPH + H+ + O2
-
738519
Gordonia sp. TY-5
methyl acetate + NADP+ + H2O
enzyme catalyzes stoichiometric conversion of acetone to methyl acetate with consumption of NADPH
-
-
?
butan-2-one + NADPH + H+ + O2
82% of the activity with acetone
738519
Gordonia sp. TY-5
methyl propanoate + NADP+ + H2O
-
-
-
?
cyclobutanone + NADPH + H+ + O2
131% of the activity with acetone
738519
Gordonia sp. TY-5
? + NADP+ + H2O
-
-
-
?
cyclohexanone + NADPH + H+ + O2
135% of the activity with acetone
738519
Gordonia sp. TY-5
? + NADP+ + H2O
-
-
-
?
cyclopentanone + NADPH + H+ + O2
170% of the activity with acetone
738519
Gordonia sp. TY-5
? + NADP+ + H2O
-
-
-
?
decan-2-one + NADPH + H+ + O2
173% of the activity with acetone
738519
Gordonia sp. TY-5
methyl nonanoate + NADP+ + H2O
-
-
-
?
heptan-2-one + NADPH + H+ + O2
80.7% of the activity with acetone
738519
Gordonia sp. TY-5
methyl hexanoate + NADP+ + H2O
-
-
-
?
nonan-2-one + NADPH + H+ + O2
186% of the activity with acetone
738519
Gordonia sp. TY-5
methyl octanoate + NADP+ + H2O
-
-
-
?
octan-2-one + NADPH + H+ + O2
128% of the activity with acetone
738519
Gordonia sp. TY-5
methyl heptanoate + NADP+ + H2O
-
-
-
?
pentan-2-one + NADPH + H+ + O2
45% of the activity with acetone
738519
Gordonia sp. TY-5
methyl butanoate + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 63000, SDS-PAGE
Gordonia sp. TY-5
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Gordonia sp. TY-5
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Gordonia sp. TY-5
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADH
Gordonia sp. TY-5
NADPH
-
Gordonia sp. TY-5
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADH
Gordonia sp. TY-5
NADPH
-
Gordonia sp. TY-5
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
4 * 63000, SDS-PAGE
Gordonia sp. TY-5
230000
-
gel filtration
Gordonia sp. TY-5
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetone + NADPH + H+ + O2
Gordonia sp. TY-5
-
methyl acetate + NADP+ + H2O
enzyme catalyzes stoichiometric conversion of acetone to methyl acetate with consumption of NADPH
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gordonia sp. TY-5
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetone + NADPH + H+ + O2
-
738519
Gordonia sp. TY-5
methyl acetate + NADP+ + H2O
enzyme catalyzes stoichiometric conversion of acetone to methyl acetate with consumption of NADPH
-
-
?
butan-2-one + NADPH + H+ + O2
82% of the activity with acetone
738519
Gordonia sp. TY-5
methyl propanoate + NADP+ + H2O
-
-
-
?
cyclobutanone + NADPH + H+ + O2
131% of the activity with acetone
738519
Gordonia sp. TY-5
? + NADP+ + H2O
-
-
-
?
cyclohexanone + NADPH + H+ + O2
135% of the activity with acetone
738519
Gordonia sp. TY-5
? + NADP+ + H2O
-
-
-
?
cyclopentanone + NADPH + H+ + O2
170% of the activity with acetone
738519
Gordonia sp. TY-5
? + NADP+ + H2O
-
-
-
?
decan-2-one + NADPH + H+ + O2
173% of the activity with acetone
738519
Gordonia sp. TY-5
methyl nonanoate + NADP+ + H2O
-
-
-
?
heptan-2-one + NADPH + H+ + O2
80.7% of the activity with acetone
738519
Gordonia sp. TY-5
methyl hexanoate + NADP+ + H2O
-
-
-
?
nonan-2-one + NADPH + H+ + O2
186% of the activity with acetone
738519
Gordonia sp. TY-5
methyl octanoate + NADP+ + H2O
-
-
-
?
octan-2-one + NADPH + H+ + O2
128% of the activity with acetone
738519
Gordonia sp. TY-5
methyl heptanoate + NADP+ + H2O
-
-
-
?
pentan-2-one + NADPH + H+ + O2
45% of the activity with acetone
738519
Gordonia sp. TY-5
methyl butanoate + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 63000, SDS-PAGE
Gordonia sp. TY-5
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Gordonia sp. TY-5
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Gordonia sp. TY-5
Other publictions for EC 1.14.13.226
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738519
Kotani
Novel acetone metabolism in a ...
Gordonia sp. TY-5
J. Bacteriol.
189
886-893
2007
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