Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-Hydroxypicolinate | stimulates rapid oxidation of NADPH | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Aminosalicylate | competitive | Pseudomonas aeruginosa | |
6-Aminonicotinate | competitive | Pseudomonas aeruginosa | |
6-Hydroxynicotinate | - |
Pseudomonas aeruginosa | |
Cl- | - |
Pseudomonas aeruginosa | |
CNS- | - |
Pseudomonas aeruginosa | |
I- | - |
Pseudomonas aeruginosa | |
N3- | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45100 | - |
2 * 45100 | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
0°C-4°C, as a precipitate under a solution of 50 mM potassium phosphate and 0.5 mM EDTA, pH 6.5-7.0, with 70% saturated ammonium sulfate, idefinitely stable | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? | |
4-mercaptobenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | 4,4'-dithiobisbenzoate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45100 | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1-deaza-FAD | when 1-deaza-FAD is used as cofactor, the enzyme carries out each step in catalysis except the transfer of oxygen to 4-hydroxybenzoate | Pseudomonas aeruginosa | |
6-Hydroxy-FAD | when 6-hydroxy-FAD is used as cofactor, the enzyme has a lower turnover rate than the native enzyme | Pseudomonas aeruginosa | |
FAD | contains 1 mol of FAD per subunit | Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |