A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis
Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.; MicrobiologyOpen 2, 471-481 (2013) 
Data extracted from this reference:
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Pantoea ananatis
expression in Escherichia coli
Pantoea ananatis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Pantoea ananatis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-isoleucine + 2-oxoglutarate + O2
Pantoea ananatis
-
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
ir
L-isoleucine + 2-oxoglutarate + O2
Pantoea ananatis AJ13355
-
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Pantoea ananatis
-
-
-
Pantoea ananatis AJ13355
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Superdex 200 HR 10/30A column
Pantoea ananatis
Storage Stability
Storage Stability
Organism
-70°C
Pantoea ananatis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
-
ir
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis AJ13355
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
-
ir
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis
31-hydroxy-L-isoleucine + succinate + CO2
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis AJ13355
31-hydroxy-L-isoleucine + succinate + CO2
-
-
-
?
L-methionine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis
L-methionine sulfoxide + succinate + CO2
-
-
-
?
L-methionine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis AJ13355
L-methionine sulfoxide + succinate + CO2
-
-
-
?
L-valine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis
4-hydroxy-L-valine + succinate + CO2
-
-
-
?
L-valine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis AJ13355
4-hydroxy-L-valine + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
HilA
-
Pantoea ananatis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pantoea ananatis
expression in Escherichia coli
Pantoea ananatis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Pantoea ananatis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-isoleucine + 2-oxoglutarate + O2
Pantoea ananatis
-
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
ir
L-isoleucine + 2-oxoglutarate + O2
Pantoea ananatis AJ13355
-
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
Superdex 200 HR 10/30A column
Pantoea ananatis
Storage Stability (protein specific)
Storage Stability
Organism
-70°C
Pantoea ananatis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
-
ir
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis AJ13355
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
-
-
-
ir
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis
31-hydroxy-L-isoleucine + succinate + CO2
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
-
754661
Pantoea ananatis AJ13355
31-hydroxy-L-isoleucine + succinate + CO2
-
-
-
?
L-methionine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis
L-methionine sulfoxide + succinate + CO2
-
-
-
?
L-methionine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis AJ13355
L-methionine sulfoxide + succinate + CO2
-
-
-
?
L-valine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis
4-hydroxy-L-valine + succinate + CO2
-
-
-
?
L-valine + 2-oxoglutarate + O2
low activity
754661
Pantoea ananatis AJ13355
4-hydroxy-L-valine + succinate + CO2
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine
Pantoea ananatis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine
Pantoea ananatis
Other publictions for EC 1.14.11.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
754661
Smirnov
A novel L-isoleucine-4'-dioxy ...
Pantoea ananatis, Pantoea ananatis AJ13355
MicrobiologyOpen
2
471-481
2013
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