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IUBMB Comments Requires Fe2+ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.
The expected taxonomic range for this enzyme is: Pantoea ananatis
Synonyms
HilA , L-isoleucine 4'-dioxygenase,
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L-isoleucine 4'-dioxygenase
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incorrect
HilA
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L-isoleucine + 2-oxoglutarate + O2 = 31-hydroxy-L-isoleucine + succinate + CO2
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L-isoleucine, 2-oxoglutarate:oxygen oxidoreductase (31-hydroxylating)
Requires Fe2+ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.
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L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
31-hydroxy-L-isoleucine + succinate + CO2
L-methionine + 2-oxoglutarate + O2
L-methionine sulfoxide + succinate + CO2
L-valine + 2-oxoglutarate + O2
4-hydroxy-L-valine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
L-isoleucine + 2-oxoglutarate + O2
31-hydroxy-L-isoleucine + succinate + CO2
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L-isoleucine + 2-oxoglutarate + O2
31-hydroxy-L-isoleucine + succinate + CO2
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L-methionine + 2-oxoglutarate + O2
L-methionine sulfoxide + succinate + CO2
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low activity
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L-methionine + 2-oxoglutarate + O2
L-methionine sulfoxide + succinate + CO2
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low activity
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L-valine + 2-oxoglutarate + O2
4-hydroxy-L-valine + succinate + CO2
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low activity
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L-valine + 2-oxoglutarate + O2
4-hydroxy-L-valine + succinate + CO2
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low activity
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L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
L-isoleucine + 2-oxoglutarate + O2
(3S)-3'-hydroxy-L-isoleucine + succinate + CO2
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ir
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brenda
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brenda
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physiological function
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enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine
physiological function
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enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine
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Superdex 200 HR 10/30A column
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expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
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Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis
MicrobiologyOpen
2
471-481
2013
Pantoea ananatis, Pantoea ananatis AJ13355
brenda
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Enzyme Nomenclature
1.14.11.74 L-isoleucine 31-dioxygenase
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