Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.70 extracted from

  • Mazmouz, R.; Chapuis-Hugon, F.; Pichon, V.; Mejean, A.; Ploux, O.
    The last step of the biosynthesis of the cyanotoxins cylindrospermopsin and 7-epi-cylindrospermopsin is catalysed by CyrI, a 2-oxoglutarate-dependent iron oxygenase (2011), ChemBioChem, 12, 858-862 .
    View publication on PubMed
Search for more literature for 1.14.11.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Oscillatoria sp. PCC 10702
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Kamptonema formosum PCC 6407
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Kamptonema sp. PCC 6506
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Oscillatoria sp. PCC 7930
expressed in Escherichia coli BL21-CodonPlus® (DE3)-RIL cells Oscillatoria sp. PCC 7926

Inhibitors

Inhibitors Comment Organism Structure
7-deoxycylindrospermopsin substrate inhibition Oscillatoria sp. PCC 7926

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 7-deoxycylindrospermopsin pH and temperature not specified in the publication Oscillatoria sp. PCC 7926

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, 0.05 mM used in assay conditions Oscillatoria sp. PCC 7926
Fe2+ dependent on, 0.05 mM used in assay conditions Oscillatoria sp. PCC 10702
Fe2+ dependent on, 0.05 mM used in assay conditions Kamptonema formosum PCC 6407
Fe2+ dependent on, 0.05 mM used in assay conditions Kamptonema sp. PCC 6506
Fe2+ dependent on, 0.05 mM used in assay conditions Oscillatoria sp. PCC 7930

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 10702
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema formosum PCC 6407
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema sp. PCC 6506
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7930
-
 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7926
-
 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 10702
-
 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema formosum PCC 6407
-
 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Kamptonema sp. PCC 6506
-
 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 Oscillatoria sp. PCC 7930
-
 cylindrospermopsin + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Kamptonema formosum PCC 6407
-
-
-
Kamptonema sp. PCC 6506
-
-
-
Oscillatoria sp. PCC 10702
-
-
-
Oscillatoria sp. PCC 7926
-
-
-
Oscillatoria sp. PCC 7930
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap Ni-affinity column chromatography Oscillatoria sp. PCC 7926
HisTrap Ni-affinity column chromatography Oscillatoria sp. PCC 10702
HisTrap Ni-affinity column chromatography Kamptonema formosum PCC 6407
HisTrap Ni-affinity column chromatography Kamptonema sp. PCC 6506
HisTrap Ni-affinity column chromatography Oscillatoria sp. PCC 7930

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 10702 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema formosum PCC 6407 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema sp. PCC 6506 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7930 7-epi-cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7926 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 10702 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema formosum PCC 6407 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Kamptonema sp. PCC 6506 cylindrospermopsin + succinate + CO2
-
 ?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
-
 Oscillatoria sp. PCC 7930 cylindrospermopsin + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 33581, quadrupole time-of-flight mass spectrometry Kamptonema sp. PCC 6506
? x * 33582, calculated from amino acid sequence Kamptonema sp. PCC 6506
? x * 33859, quadrupole time-of-flight mass spectrometry Oscillatoria sp. PCC 7926
? x * 33860, calculated from amino acid sequence Oscillatoria sp. PCC 7926

Synonyms

Synonyms Comment Organism
cyrI
-
 Oscillatoria sp. PCC 7926
cyrI
-
 Oscillatoria sp. PCC 10702
cyrI
-
 Kamptonema formosum PCC 6407
cyrI
-
 Kamptonema sp. PCC 6506
cyrI
-
 Oscillatoria sp. PCC 7930

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.036
-
 7-deoxycylindrospermopsin pH and temperature not specified in the publication Oscillatoria sp. PCC 7926

General Information

General Information Comment Organism
metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Oscillatoria sp. PCC 7926
metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Oscillatoria sp. PCC 10702
metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Kamptonema formosum PCC 6407
metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Kamptonema sp. PCC 6506
metabolism the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin Oscillatoria sp. PCC 7930