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IUBMB Comments Requires Fe(II). The enzyme, found in some cyanobacterial species, catalyses the last step in the biosynthesis of the toxins cylindrospermopsin and 7-epi-cylindrospermopsin. The ratio of the two products differs among different strains.
The enzyme appears in viruses and cellular organisms
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cyrI
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7-deoxycylindrospermopsin + 2-oxoglutarate + O2 = 7-epi-cylindrospermopsin + succinate + CO2
(2)
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7-deoxycylindrospermopsin + 2-oxoglutarate + O2 = cylindrospermopsin + succinate + CO2
(1)
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7-deoxycylindrospermopsin,2-oxoglutarate:oxygen oxidoreductase (7-hydroxylating)
Requires Fe(II). The enzyme, found in some cyanobacterial species, catalyses the last step in the biosynthesis of the toxins cylindrospermopsin and 7-epi-cylindrospermopsin. The ratio of the two products differs among different strains.
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7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
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-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
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-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
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-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
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-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
?
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7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
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-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
-
-
-
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?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
7-epi-cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
-
?
7-deoxycylindrospermopsin + 2-oxoglutarate + O2
cylindrospermopsin + succinate + CO2
-
-
-
-
?
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Fe2+
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dependent on, 0.05 mM used in assay conditions
Fe2+
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dependent on, 0.05 mM used in assay conditions
Fe2+
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dependent on, 0.05 mM used in assay conditions
Fe2+
-
dependent on, 0.05 mM used in assay conditions
Fe2+
-
dependent on. Km value is 0.0021 mM. 0.05 mM used in assay conditions
Fe2+
-
dependent on, 0.05 mM used in assay conditions
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7-deoxycylindrospermopsin
7-deoxycylindrospermopsin
-
the enzyme is strongly inhibited by 7-deoxycylindrospermopsin at concentration higher than 0.001 mM
7-deoxycylindrospermopsin
-
substrate inhibition
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0.0032
2-oxoglutarate
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pH 7.4 and 28°C
0.0012 - 0.019
7-deoxycylindrospermopsin
0.0012
7-deoxycylindrospermopsin
-
pH and temperature not specified in the publication
0.019
7-deoxycylindrospermopsin
-
pH 7.4 and 28°C
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0.03
2-oxoglutarate
-
pH 7.4 and 28°C
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38.3
7-deoxycylindrospermopsin
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pH 7.4 and 28°C
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0.036
7-deoxycylindrospermopsin
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pH and temperature not specified in the publication
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brenda
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brenda
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brenda
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brenda
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brenda
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metabolism
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the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin
metabolism
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the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin
metabolism
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the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin
metabolism
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the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin
metabolism
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the enzyme catalyses the last step of the biosynthesis of the cyanobacterial cytotoxin cylindrospermopsin
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?
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x * 33581, quadrupole time-of-flight mass spectrometry
?
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x * 33582, calculated from amino acid sequence
?
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x * 33859, quadrupole time-of-flight mass spectrometry
?
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x * 33860, calculated from amino acid sequence
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His-Trap nickel column chromatography and Superdex S200 gel filtration
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HisTrap Ni-affinity column chromatography
HisTrap Ni-affinity column chromatography
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HisTrap Ni-affinity column chromatography
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HisTrap Ni-affinity column chromatography
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HisTrap Ni-affinity column chromatography
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HisTrap Ni-affinity column chromatography
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
expressed in Escherichia coli BL21-CodonPlus® (DE3)-RIL cells
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
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Mazmouz, R.; Essadik, I.; Hamdane, D.; Mejean, A.; Ploux, O.
Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis Binding studies, site-directed mutagenesis and stereoselectivity
Arch. Biochem. Biophys.
647
1-9
2018
Oscillatoria sp. PCC 7926
brenda
Mazmouz, R.; Chapuis-Hugon, F.; Pichon, V.; Mejean, A.; Ploux, O.
The last step of the biosynthesis of the cyanotoxins cylindrospermopsin and 7-epi-cylindrospermopsin is catalysed by CyrI, a 2-oxoglutarate-dependent iron oxygenase
ChemBioChem
12
858-862
2011
Oscillatoria sp. PCC 7926, Oscillatoria sp. PCC 10702, Kamptonema formosum PCC 6407, Kamptonema sp. PCC 6506, Oscillatoria sp. PCC 7930
brenda
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Enzyme Nomenclature
1.14.11.70 7-deoxycylindrospermopsin hydroxylase
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