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Literature summary for 1.14.11.45 extracted from
- 4-Hydroxyisoleucine production of recombinant Corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation (2015), Appl. Microbiol. Biotechnol., 99, 3851-3863 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells and Corynebacterium glutamicum ssp. lactofermentum strain SN01 | Bacillus thuringiensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
2-oxoglutarate | at pH 6.0 and 40°C | Bacillus thuringiensis |  |
| 0.61 | - |
L-isoleucine | at pH 6.0 and 40°C | Bacillus thuringiensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis | - |
(4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
| L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis YBT-1520 | - |
(4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis | - |
- |
- |
| Bacillus thuringiensis YBT-1520 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Bacillus thuringiensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:corn steep liquor-grown cell | - |
Bacillus thuringiensis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, purified enzyme, 1 h and 4 h, 18% and more than 70% loss of activity, respectively | Bacillus thuringiensis |
| 4°C, purified enzyme, 2 and 8 days, 30% and more than 85% loss of activity, respectively | Bacillus thuringiensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
| L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis YBT-1520 | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Bacillus thuringiensis |
| ? | x * 29550, calculated from amino acid sequence | Bacillus thuringiensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDO | - |
Bacillus thuringiensis |
| L-isoleucine dioxygenase | - |
Bacillus thuringiensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus thuringiensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | preincubating the enzyme at 50°C for 1 h results in a loss of approximately half of activity, and nearly no activity is detected after preheating at 70°C for 1 h | Bacillus thuringiensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
2-oxoglutarate | at pH 6.0 and 40°C | Bacillus thuringiensis | |
| 0.089 | - |
L-isoleucine | at pH 6.0 and 40°C | Bacillus thuringiensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus thuringiensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7 | about 25% activity at pH 5.5 and 7.0, respectively, about 50% activity at pH 6.5 | Bacillus thuringiensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | the enzyme remains stable between pH 5.0 and 8.0 for 24 h | Bacillus thuringiensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.116 | - |
2-oxoglutarate | at pH 6.0 and 40°C | Bacillus thuringiensis | |
| 0.146 | - |
L-isoleucine | at pH 6.0 and 40°C | Bacillus thuringiensis | |
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