Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cannabidiol | CBD, a major non-psychotropic cannabis constituent, stimulates lysyl hydroxylase 1 activity in osteoblasts, cannabidiol enhances the biomechanical properties of healing rat mid-femoral fractures | Rattus norvegicus | |
DELTA9-tetrahydrocannabinol | THC, the psychotropic cannabis constituent, stimulates lysyl hydroxylase 2 activity in osteoblasts | Rattus norvegicus | |
additional information | no stimulating effect of LH1 by DELTA9-tetrahydrocannabinol (THC), but THC potentiates the CBD stimulated work-to-failure at 6 weeks postfracture followed by attenuation of the CBD effect at 8 weeks | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
gene Plod1, real-time RT-PCR enzyme expression analysis | Rattus norvegicus |
gene Plod2, real-time RT-PCR enzyme expression analysis | Rattus norvegicus |
gene Plod3, real-time RT-PCR enzyme expression analysis | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[procollagen]-L-lysine + 2-oxoglutarate + O2 | Rattus norvegicus | - |
[procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q5U367 | - |
- |
Rattus norvegicus | Q63321 | - |
- |
Rattus norvegicus | Q811A3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | - |
Rattus norvegicus | - |
osteoblast | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[procollagen]-L-lysine + 2-oxoglutarate + O2 | - |
Rattus norvegicus | [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LH1 | - |
Rattus norvegicus |
LH2 | - |
Rattus norvegicus |
LH3 | - |
Rattus norvegicus |
Plod1 | - |
Rattus norvegicus |
PLOD2 | - |
Rattus norvegicus |
PLOD3 | - |
Rattus norvegicus |
Organism | Comment | Expression |
---|---|---|
Rattus norvegicus | no induction of PLOD3 expression in osteoblasts by DELTA9-tetrahydrocannabinol and cannabidiol | additional information |
Rattus norvegicus | cannabidiol stimulates mRNA expression of Plod1 in primary osteoblast cultures | up |
Rattus norvegicus | DELTA9-tetrahydrocannabinol stimulates mRNA expression of Plod2 in primary osteoblast cultures | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes lysine hydroxylation, which is involved in collagen crosslinking and stabilization. Critical mechanical role of collagen crosslinking enzymes, e.g. in bone healing. Lysyl hydroxylase 1 (PLOD1) is one of the few collagen crosslinking enzymes associated with bone quality | Rattus norvegicus |
physiological function | the enzyme LH2 catalyzes lysine hydroxylation, which is involved in collagen crosslinking and stabilization. PLOD2 has no role in determining bone quality | Rattus norvegicus |
physiological function | the enzyme LH3 catalyzes lysine hydroxylation | Rattus norvegicus |