BRENDA - Enzyme Database show
show all sequences of 1.14.11.28

Purification and cloning of a proline 3-hydroxylase, a novel enzyme which hydroxylates free L-proline to cis-3-hydroxy-L-proline

Mori, H.; Shibasaki, T.; Yano, K.; Ozaki, A.; J. Bacteriol. 179, 5677-5683 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
L-ascorbate
2 mM, no further stimulation above 2 mM
Streptomyces sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Citric acid
30% inhibition at 5 mM
Streptomyces sp.
Co2+
more than 90% inhibition at 1 mM
Streptomyces sp.
Cu2+
more than 90% inhibition at 1 mM
Streptomyces sp.
EDTA
95% inhibition at 2 mM
Streptomyces sp.
Zn2+
more than 90% inhibition at 1 mM
Streptomyces sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
2-oxoglutarate
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
0.56
-
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
strictly required, optimal stimulation at 1 mM
Streptomyces sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
SDS-PAGE
Streptomyces sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
-
strainTH1
-
Streptomyces sp. TH1
-
strainTH1
-
Purification (Commentary)
Commentary
Organism
5 chromatographic steps near to homogeneity
Streptomyces sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Streptomyces sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-proline + 2-oxoglutarate + O2
-
665361
Streptomyces sp.
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
-
665361
Streptomyces sp. TH1
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Streptomyces sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2
-
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Streptomyces sp.
Cofactor
Cofactor
Commentary
Organism
Structure
2-oxoglutarate
strictly required, highest activity at 4 mM
Streptomyces sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces sp.
-
-
4.3
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
L-ascorbate
2 mM, no further stimulation above 2 mM
Streptomyces sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2-oxoglutarate
strictly required, highest activity at 4 mM
Streptomyces sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Citric acid
30% inhibition at 5 mM
Streptomyces sp.
Co2+
more than 90% inhibition at 1 mM
Streptomyces sp.
Cu2+
more than 90% inhibition at 1 mM
Streptomyces sp.
EDTA
95% inhibition at 2 mM
Streptomyces sp.
Zn2+
more than 90% inhibition at 1 mM
Streptomyces sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
2-oxoglutarate
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
0.56
-
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
strictly required, optimal stimulation at 1 mM
Streptomyces sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
SDS-PAGE
Streptomyces sp.
Purification (Commentary) (protein specific)
Commentary
Organism
5 chromatographic steps near to homogeneity
Streptomyces sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Streptomyces sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-proline + 2-oxoglutarate + O2
-
665361
Streptomyces sp.
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
-
665361
Streptomyces sp. TH1
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Streptomyces sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2
-
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Streptomyces sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces sp.
-
-
4.3
Other publictions for EC 1.14.11.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727259
Hatzimichael
The collagen prolyl hydroxylas ...
Homo sapiens
Br. J. Cancer
107
1423-1432
2012
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-
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-
-
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2
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1
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1
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1
-
-
1
-
-
726654
Mordechai
High myopia caused by a mutati ...
Homo sapiens
Am. J. Hum. Genet.
89
438-445
2011
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-
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-
1
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1
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1
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697017
Shah
The prolyl 3-hydroxylases P3H2 ...
Homo sapiens
Br. J. Cancer
100
1687-1696
2009
-
1
-
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2
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2
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1
1
2
2
-
-
697667
Johnston
-
Hydroxylation of L-proline to ...
Streptomyces sp., Streptomyces sp. TH1
Enzyme Microb. Technol.
45
484-490
2009
-
-
1
-
-
-
-
-
-
1
1
2
-
7
-
-
-
-
-
-
1
-
4
1
1
-
-
-
1
-
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-
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-
1
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-
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-
-
-
-
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-
1
1
2
-
-
-
-
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
665517
Vranka
Prolyl 3-hydroxylase 1, enzyme ...
Gallus gallus
J. Biol. Chem.
279
23615-23621
2004
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
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-
1
-
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1
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1
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1
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-
-
-
-
-
-
664814
Clifton
Structure of proline 3-hydroxy ...
Streptomyces sp., Streptomyces sp. TH1
Eur. J. Biochem.
268
6625-6636
2001
-
-
-
1
-
-
-
-
-
-
-
2
-
8
-
-
1
-
-
1
-
-
2
1
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-
1
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2
-
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-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664403
Shibasaki
-
Cloning of an isozyme of proli ...
Streptomyces sp., Streptomyces sp. TH1
Biotechnol. Lett.
22
1967-1973
2000
-
-
1
-
-
-
5
4
-
1
3
-
-
7
-
-
1
-
-
1
2
-
8
-
2
-
2
-
1
-
2
1
-
2
-
-
-
1
1
-
-
-
-
5
-
4
-
1
3
-
-
-
-
1
-
1
2
-
8
-
2
-
2
-
1
-
2
2
-
-
-
-
-
-
666959
Shibasaki
-
Substrate selectivities of pro ...
Streptomyces sp., Streptomyces sp. TH1
Tetrahedron Lett.
40
5227-5230
1999
-
-
1
-
-
-
-
6
-
-
-
-
-
7
-
-
1
-
-
1
-
-
8
-
-
-
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-
-
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1
-
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-
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6
-
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-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665361
Mori
Purification and cloning of a ...
Streptomyces sp., Streptomyces sp. TH1
J. Bacteriol.
179
5677-5683
1997
1
-
-
-
-
-
5
2
-
1
1
-
-
10
-
-
1
-
-
1
-
-
2
-
1
-
-
1
1
-
-
1
-
1
-
1
-
-
1
-
-
-
-
5
-
2
-
1
1
-
-
-
-
1
-
1
-
-
2
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
663681
Mori
Detection of novel proline 3-h ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) TH2, Bacillus sp. (in: Bacteria) TH3, Streptomyces canus, Streptomyces sp., Streptomyces sp. TH1
Appl. Environ. Microbiol.
62
1903-1907
1996
-
-
-
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4
-
-
3
-
-
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16
-
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-
3
4
-
6
-
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-
-
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3
-
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-
3
-
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4
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3
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-
3
4
-
6
-
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