Data extracted from this reference:
Activating Compound
L-ascorbate
2 mM, no further stimulation above 2 mM
Streptomyces sp.
Inhibitors
Citric acid
30% inhibition at 5 mM
Streptomyces sp.
Co2+
more than 90% inhibition at 1 mM
Streptomyces sp.
Cu2+
more than 90% inhibition at 1 mM
Streptomyces sp.
EDTA
95% inhibition at 2 mM
Streptomyces sp.
Zn2+
more than 90% inhibition at 1 mM
Streptomyces sp.
KM Value [mM]
0.11
2-oxoglutarate
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
0.56
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
Metals/Ions
Fe2+
strictly required, optimal stimulation at 1 mM
Streptomyces sp.
Molecular Weight [Da]
35000
SDS-PAGE
Streptomyces sp.
Organism
Streptomyces sp.
strainTH1
Streptomyces sp. TH1
strainTH1
Purification (Commentary)
5 chromatographic steps near to homogeneity
Streptomyces sp.
Source Tissue
cell culture
Streptomyces sp.
Substrates and Products (Substrate)
L-proline + 2-oxoglutarate + O2
665361
Streptomyces sp.
cis-3-hydroxy-L-proline + succinate + CO2
?
L-proline + 2-oxoglutarate + O2
665361
Streptomyces sp. TH1
cis-3-hydroxy-L-proline + succinate + CO2
?
Synonyms
proline 3-hydroxylase
Streptomyces sp.
Temperature Optimum [°C]
Turnover Number [1/s]
3.2
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
pH Optimum
Cofactor
2-oxoglutarate
strictly required, highest activity at 4 mM
Streptomyces sp.
pI Value
Activating Compound (protein specific)
L-ascorbate
2 mM, no further stimulation above 2 mM
Streptomyces sp.
Cofactor (protein specific)
2-oxoglutarate
strictly required, highest activity at 4 mM
Streptomyces sp.
Inhibitors (protein specific)
Citric acid
30% inhibition at 5 mM
Streptomyces sp.
Co2+
more than 90% inhibition at 1 mM
Streptomyces sp.
Cu2+
more than 90% inhibition at 1 mM
Streptomyces sp.
EDTA
95% inhibition at 2 mM
Streptomyces sp.
Zn2+
more than 90% inhibition at 1 mM
Streptomyces sp.
KM Value [mM] (protein specific)
0.11
2-oxoglutarate
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
0.56
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
Metals/Ions (protein specific)
Fe2+
strictly required, optimal stimulation at 1 mM
Streptomyces sp.
Molecular Weight [Da] (protein specific)
35000
SDS-PAGE
Streptomyces sp.
Purification (Commentary) (protein specific)
5 chromatographic steps near to homogeneity
Streptomyces sp.
Source Tissue (protein specific)
cell culture
Streptomyces sp.
Substrates and Products (Substrate) (protein specific)
L-proline + 2-oxoglutarate + O2
665361
Streptomyces sp.
cis-3-hydroxy-L-proline + succinate + CO2
?
L-proline + 2-oxoglutarate + O2
665361
Streptomyces sp. TH1
cis-3-hydroxy-L-proline + succinate + CO2
?
Temperature Optimum [°C] (protein specific)
Turnover Number [1/s] (protein specific)
3.2
L-proline
100 mM N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid pH 7.0, 5 mM 2-oxoglutarate, 5 mM L-proline, 5 mM L-ascorbate, 1 mM FeSO4, 35°C
Streptomyces sp.
pH Optimum (protein specific)
pI Value (protein specific)
Other publictions for EC
727259
Hatzimichael
The collagen prolyl hydroxylas ...
Homo sapiens
Br. J. Cancer
107
1423-1432
2012
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726654
Mordechai
High myopia caused by a mutati ...
Homo sapiens
Am. J. Hum. Genet.
89
438-445
2011
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697017
Shah
The prolyl 3-hydroxylases P3H2 ...
Homo sapiens
Br. J. Cancer
100
1687-1696
2009
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1
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1
1
2
2
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697667
Johnston
-
Hydroxylation of L-proline to ...
Streptomyces sp., Streptomyces sp. TH1
Enzyme Microb. Technol.
45
484-490
2009
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1
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1
1
2
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7
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1
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1
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1
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4
1
1
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1
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665517
Vranka
Prolyl 3-hydroxylase 1, enzyme ...
Gallus gallus
J. Biol. Chem.
279
23615-23621
2004
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664814
Clifton
Structure of proline 3-hydroxy ...
Streptomyces sp., Streptomyces sp. TH1
Eur. J. Biochem.
268
6625-6636
2001
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1
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2
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8
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1
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1
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2
1
2
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1
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2
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1
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1
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2
1
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664403
Shibasaki
-
Cloning of an isozyme of proli ...
Streptomyces sp., Streptomyces sp. TH1
Biotechnol. Lett.
22
1967-1973
2000
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1
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5
4
-
1
3
-
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7
-
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1
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1
2
-
8
-
2
2
-
2
-
1
-
2
1
-
2
-
-
-
1
1
-
-
-
-
5
-
4
-
1
3
-
-
-
-
1
-
1
2
-
8
-
2
-
2
-
1
-
2
2
-
-
-
-
-
-
666959
Shibasaki
-
Substrate selectivities of pro ...
Streptomyces sp., Streptomyces sp. TH1
Tetrahedron Lett.
40
5227-5230
1999
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1
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6
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7
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1
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1
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8
-
2
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-
-
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-
1
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-
-
-
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6
-
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-
-
-
-
1
-
1
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8
-
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-
-
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665361
Mori
Purification and cloning of a ...
Streptomyces sp., Streptomyces sp. TH1
J. Bacteriol.
179
5677-5683
1997
1
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5
2
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1
1
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-
11
-
-
1
-
-
1
-
-
2
-
1
1
-
-
1
1
-
-
1
-
1
-
1
-
-
1
-
-
-
-
5
-
2
-
1
1
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1
-
1
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-
2
-
1
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1
1
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-
1
-
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-
-
-
-
663681
Mori
Detection of novel proline 3-h ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) TH2, Bacillus sp. (in: Bacteria) TH3, Streptomyces canus, Streptomyces sp., Streptomyces sp. TH1
Appl. Environ. Microbiol.
62
1903-1907
1996
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4
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3
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16
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3
4
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6
-
3
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3
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3
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4
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3
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3
4
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6
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