BRENDA - Enzyme Database show
show all sequences of 1.14.11.21

Purification and characterization of clavaminate synthase from Streptomyces antibioticus. A multifunctional enzyme of clavam biosynthesis

Janc, J.W.; Egan, L.A.; Townsend, C.A.; J. Biol. Chem. 270, 5399-5404 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0032
-
Fe2+
pH 7.0, 22°C
Streptomyces antibioticus
0.039
-
2-oxoglutarate
pH 7.0, 22°C
Streptomyces antibioticus
0.38
-
(2S,3R)-proclavaminate
pH 7.0, 22°C
Streptomyces antibioticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Streptomyces antibioticus
Fe2+
required
Streptomyces clavuligerus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35360
-
CS1, mass spectrometry
Streptomyces clavuligerus
35750
-
CS2, mass spectrometry
Streptomyces clavuligerus
35840
-
CS3, mass spectrometry
Streptomyces antibioticus
38000
-
CS1, gel filtration
Streptomyces clavuligerus
41000
-
CS3, gel filtration
Streptomyces antibioticus
42000
-
CS2, gel filtration
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
amidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
amidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus Tu1718
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus Tu1718
?
dihydroclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
clavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
dihydroclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
clavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
additional information
Streptomyces antibioticus
enzyme plays a critical role in the biosynthesis of the clavam metabolites
?
-
-
-
additional information
Streptomyces clavuligerus
enzyme plays a critical role in the biosynthesis of the clavam metabolites
?
-
-
-
additional information
Streptomyces antibioticus
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
?
-
-
-
additional information
Streptomyces clavuligerus
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
?
-
-
-
additional information
Streptomyces antibioticus Tu1718
enzyme plays a critical role in the biosynthesis of the clavam metabolites
?
-
-
-
additional information
Streptomyces antibioticus Tu1718
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
?
-
-
-
proclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
dihydroclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
dihydroclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces antibioticus
-
strain Tü 1718, CS3, no production of clavulanic acid
-
Streptomyces antibioticus Tu1718
-
strain Tü 1718, CS3, no production of clavulanic acid
-
Streptomyces clavuligerus
-
2 isozymes CS1 and CS2
-
Oxidation Stability
Oxidation Stability
Organism
inactivation due to release of peroxide from reaction of 2-oxoglutarate and O2 in absence of proclavaminate, in presence of Fe2+, t1/2: 52 s, catalase partially protects
Streptomyces clavuligerus
Purification (Commentary)
Commentary
Organism
12.5fold to homogeneity
Streptomyces antibioticus
Reaction
Reaction
Commentary
Organism
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2
reaction mechanism
Streptomyces antibioticus
deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2
reaction mechanism
Streptomyces clavuligerus
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O
reaction mechanism
Streptomyces antibioticus
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O
reaction mechanism
Streptomyces clavuligerus
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O
reaction mechanism
Streptomyces antibioticus
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O
reaction mechanism
Streptomyces clavuligerus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.1
-
purified enzyme
Streptomyces antibioticus
Storage Stability
Storage Stability
Organism
-80°C, purified protein, 50 mM MOPS, pH 7.0, 0.02 mM EDTA, 2 mM DTT, 50% glycerol, stable for at least 1 year
Streptomyces antibioticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + O2
side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme
639274
Streptomyces antibioticus
? + H2O2
-
-
-
?
2-oxoglutarate + O2
side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme
639274
Streptomyces clavuligerus
? + H2O2
-
-
-
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
amidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
amidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639274
Streptomyces antibioticus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639274
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus Tu1718
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus Tu1718
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639274
Streptomyces antibioticus Tu1718
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus Tu1718
?
dihydroclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
dihydroclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
cyclization
639274
Streptomyces antibioticus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
dihydroclavaminate + 2-oxoglutarate + O2
cyclization
639274
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
additional information
dependent on O2 and 2-oxoglutarate
639274
Streptomyces antibioticus
?
-
-
-
-
additional information
dependent on O2 and 2-oxoglutarate
639274
Streptomyces clavuligerus
?
-
-
-
-
additional information
enzyme plays a critical role in the biosynthesis of the clavam metabolites
639274
Streptomyces antibioticus
?
-
-
-
-
additional information
enzyme plays a critical role in the biosynthesis of the clavam metabolites
639274
Streptomyces clavuligerus
?
-
-
-
-
additional information
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
639274
Streptomyces antibioticus
?
-
-
-
-
additional information
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
639274
Streptomyces clavuligerus
?
-
-
-
-
additional information
dependent on O2 and 2-oxoglutarate
639274
Streptomyces antibioticus Tu1718
?
-
-
-
-
additional information
enzyme plays a critical role in the biosynthesis of the clavam metabolites
639274
Streptomyces antibioticus Tu1718
?
-
-
-
-
additional information
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
639274
Streptomyces antibioticus Tu1718
?
-
-
-
-
proclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
saturation
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
saturation
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
no activity with the racemic erythro isomers (2S,3S) and (2R,3R)
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
no activity with the racemic erythro isomers (2S,3S) and (2R,3R)
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
specific for the threo-(2S,3R) enantiomer
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
specific for the threo-(2S,3R) enantiomer
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 35839-38000, CS3, mass spectrometry and SDS-PAGE
Streptomyces antibioticus
monomer
1 * 35357-45000, CS1, mass spectrometry and SDS-PAGE; 1 * 35750-44000, CS2, mass spectrometry and SDS-PAGE
Streptomyces clavuligerus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Streptomyces antibioticus
7
-
assay at
Streptomyces clavuligerus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0032
-
Fe2+
pH 7.0, 22°C
Streptomyces antibioticus
0.039
-
2-oxoglutarate
pH 7.0, 22°C
Streptomyces antibioticus
0.38
-
(2S,3R)-proclavaminate
pH 7.0, 22°C
Streptomyces antibioticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Streptomyces antibioticus
Fe2+
required
Streptomyces clavuligerus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35360
-
CS1, mass spectrometry
Streptomyces clavuligerus
35750
-
CS2, mass spectrometry
Streptomyces clavuligerus
35840
-
CS3, mass spectrometry
Streptomyces antibioticus
38000
-
CS1, gel filtration
Streptomyces clavuligerus
41000
-
CS3, gel filtration
Streptomyces antibioticus
42000
-
CS2, gel filtration
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
amidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
amidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus Tu1718
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus Tu1718
?
dihydroclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
clavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
dihydroclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
clavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
additional information
Streptomyces antibioticus
enzyme plays a critical role in the biosynthesis of the clavam metabolites
?
-
-
-
additional information
Streptomyces clavuligerus
enzyme plays a critical role in the biosynthesis of the clavam metabolites
?
-
-
-
additional information
Streptomyces antibioticus
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
?
-
-
-
additional information
Streptomyces clavuligerus
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
?
-
-
-
additional information
Streptomyces antibioticus Tu1718
enzyme plays a critical role in the biosynthesis of the clavam metabolites
?
-
-
-
additional information
Streptomyces antibioticus Tu1718
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
?
-
-
-
proclavaminate + 2-oxoglutarate + O2
Streptomyces antibioticus
-
dihydroclavaminate + succinate + CO2 + H2O
-
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
dihydroclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
inactivation due to release of peroxide from reaction of 2-oxoglutarate and O2 in absence of proclavaminate, in presence of Fe2+, t1/2: 52 s, catalase partially protects
Streptomyces clavuligerus
Purification (Commentary) (protein specific)
Commentary
Organism
12.5fold to homogeneity
Streptomyces antibioticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.1
-
purified enzyme
Streptomyces antibioticus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, purified protein, 50 mM MOPS, pH 7.0, 0.02 mM EDTA, 2 mM DTT, 50% glycerol, stable for at least 1 year
Streptomyces antibioticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + O2
side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme
639274
Streptomyces antibioticus
? + H2O2
-
-
-
?
2-oxoglutarate + O2
side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme
639274
Streptomyces clavuligerus
? + H2O2
-
-
-
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
amidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
amidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639274
Streptomyces antibioticus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639274
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus Tu1718
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus Tu1718
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639274
Streptomyces antibioticus Tu1718
guanidinoproclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus Tu1718
?
dihydroclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
dihydroclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
cyclization
639274
Streptomyces antibioticus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
dihydroclavaminate + 2-oxoglutarate + O2
cyclization
639274
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
additional information
dependent on O2 and 2-oxoglutarate
639274
Streptomyces antibioticus
?
-
-
-
-
additional information
dependent on O2 and 2-oxoglutarate
639274
Streptomyces clavuligerus
?
-
-
-
-
additional information
enzyme plays a critical role in the biosynthesis of the clavam metabolites
639274
Streptomyces antibioticus
?
-
-
-
-
additional information
enzyme plays a critical role in the biosynthesis of the clavam metabolites
639274
Streptomyces clavuligerus
?
-
-
-
-
additional information
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
639274
Streptomyces antibioticus
?
-
-
-
-
additional information
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
639274
Streptomyces clavuligerus
?
-
-
-
-
additional information
dependent on O2 and 2-oxoglutarate
639274
Streptomyces antibioticus Tu1718
?
-
-
-
-
additional information
enzyme plays a critical role in the biosynthesis of the clavam metabolites
639274
Streptomyces antibioticus Tu1718
?
-
-
-
-
additional information
biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor
639274
Streptomyces antibioticus Tu1718
?
-
-
-
-
proclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
-
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
saturation
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
saturation
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
no activity with the racemic erythro isomers (2S,3S) and (2R,3R)
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
no activity with the racemic erythro isomers (2S,3S) and (2R,3R)
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
specific for the threo-(2S,3R) enantiomer
639274
Streptomyces antibioticus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces antibioticus
?
proclavaminate + 2-oxoglutarate + O2
specific for the threo-(2S,3R) enantiomer
639274
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639274
Streptomyces clavuligerus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 35839-38000, CS3, mass spectrometry and SDS-PAGE
Streptomyces antibioticus
monomer
1 * 35357-45000, CS1, mass spectrometry and SDS-PAGE; 1 * 35750-44000, CS2, mass spectrometry and SDS-PAGE
Streptomyces clavuligerus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Streptomyces antibioticus
7
-
assay at
Streptomyces clavuligerus
Other publictions for EC 1.14.11.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742159
Shrestha
-
Heterologous production of cl ...
Streptomyces clavuligerus
Biotechnol. Bioprocess Eng.
22
359-365
2017
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723902
Chin
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Predicting the catalytic sites ...
Streptomyces clavuligerus
Afr. J. Microbiol. Res.
5
3357-3366
2011
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685142
Borowski
Mechanism for cyclization reac ...
Streptomyces clavuligerus
Biochemistry
46
3682-3691
2007
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639036
Townsend
New reactions in clavulanic ac ...
Streptomyces clavuligerus
Curr. Opin. Chem. Biol.
6
583-589
2002
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639286
Zhang
Crystal structure of a clavami ...
Streptomyces clavuligerus
FEBS Lett.
517
7-12
2002
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639284
Zhou
Spectroscopic studies of subst ...
Streptomyces clavuligerus
J. Am. Chem. Soc.
123
7388-7398
2001
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639281
Zhang
Structural origins of the sele ...
Streptomyces clavuligerus
Nat. Struct. Biol.
7
127-133
2000
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639282
Khaleeli
Site-directed mutagenesis and ...
Streptomyces clavuligerus
Biochemistry
39
8666-8673
2000
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8
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639283
Doan
Mutagenesis studies on the iro ...
Streptomyces clavuligerus
Biochem. Biophys. Res. Commun.
279
240-244
2000
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15
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639280
Lloyd
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Product-substrate engineering ...
Streptomyces clavuligerus
Tetrahedron
55
10201-10220
1999
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639279
Zhou
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Substrate binding to the alpha ...
Streptomyces clavuligerus
J. Am. Chem. Soc.
120
13539-13540
1998
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639278
Baldwin
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Chemo-enzymic synthesis of bic ...
Streptomyces clavuligerus
Tetrahedron
53
7011-7020
1997
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639277
Busby
A single monomeric iron center ...
Streptomyces clavuligerus
Bioorg. Med. Chem.
4
1059-1064
1996
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639274
Janc
Purification and characterizat ...
Streptomyces antibioticus, Streptomyces antibioticus Tu1718, Streptomyces clavuligerus
J. Biol. Chem.
270
5399-5404
1995
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639275
Busby
Expression and purification of ...
Streptomyces clavuligerus
J. Biol. Chem.
270
4262-4269
1995
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639276
Paradkar
Functional analysis of the gen ...
Streptomyces clavuligerus, Streptomyces clavuligerus NRRL 3585
J. Bacteriol.
177
1307-1314
1995
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639273
Iwata-Reuyl
Synthesis and reaction of pote ...
Streptomyces clavuligerus
J. Nat. Prod.
56
1373-1396
1993
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639272
Elson
-
Studies on the substrate speci ...
Streptomyces clavuligerus
Bioorg. Med. Chem. Lett.
2
1503-1508
1992
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639271
Salowe
Elucidation of the order of ox ...
Streptomyces clavuligerus
Biochemistry
30
2281-2292
1991
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639270
Salowe
Purification and characterizat ...
Streptomyces clavuligerus
Biochemistry
29
6499-6508
1990
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