BRENDA - Enzyme Database show
show all sequences of 1.14.11.16

Site-directed mutagenesis of residues in a conserved region of bovine aspartyl (asparaginyl) beta-hydroxylase: Evidence that histidine 675 has a role in binding Fe2+

McGinnis, K.; Ku, G.M.; VanDusen, W.J.; Fu, J.; Garsky, V.; Stern, A.M.; Friedman, P.A.; Biochemistry 35, 3957-3962 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
wild-type and mutants expressed in Escherichia coli BL21
Bos taurus
Engineering
Amino acid exchange
Commentary
Organism
G659A
21% activity of wild-type
Bos taurus
G669A
90% activity of wild-type
Bos taurus
H667L
106% activity of wild-type
Bos taurus
H671L
16% activity of wild-type
Bos taurus
H675D
20% activity of wild-type
Bos taurus
H675E
12% activity of wild-type
Bos taurus
H686L
9% activity of wild-type
Bos taurus
P678V
90% activity of wild-type
Bos taurus
R682A
10% activity of wild-type
Bos taurus
R684A
8% activity of wild-type
Bos taurus
R684K
87% activity of wild-type
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
Fe2+
at 0.6 mM 2-oxoglutarate, wild-type
Bos taurus
0.052
-
Fe2+
at 0.6 mM 2-oxoglutarate, H675E mutant
Bos taurus
0.093
-
Fe2+
at 0.6 mM 2-oxoglutarate, H675D mutant
Bos taurus
0.098
-
2-oxoglutarate
at 1 mM Fe2+, H675D mutant
Bos taurus
0.102
-
2-oxoglutarate
at 1 mM Fe2+, wild-type
Bos taurus
0.125
-
2-oxoglutarate
at 1 mM Fe2+, H675E mutant
Bos taurus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
peptide L-aspartate + 2-oxoglutarate + O2
second epidermal growth factor-like domain of bovine protein S
439255
Bos taurus
peptide 3-hydroxy-L-aspartate + succinate + CO2
-
439255
Bos taurus
?
Cloned(Commentary) (protein specific)
Commentary
Organism
wild-type and mutants expressed in Escherichia coli BL21
Bos taurus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
G659A
21% activity of wild-type
Bos taurus
G669A
90% activity of wild-type
Bos taurus
H667L
106% activity of wild-type
Bos taurus
H671L
16% activity of wild-type
Bos taurus
H675D
20% activity of wild-type
Bos taurus
H675E
12% activity of wild-type
Bos taurus
H686L
9% activity of wild-type
Bos taurus
P678V
90% activity of wild-type
Bos taurus
R682A
10% activity of wild-type
Bos taurus
R684A
8% activity of wild-type
Bos taurus
R684K
87% activity of wild-type
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
Fe2+
at 0.6 mM 2-oxoglutarate, wild-type
Bos taurus
0.052
-
Fe2+
at 0.6 mM 2-oxoglutarate, H675E mutant
Bos taurus
0.093
-
Fe2+
at 0.6 mM 2-oxoglutarate, H675D mutant
Bos taurus
0.098
-
2-oxoglutarate
at 1 mM Fe2+, H675D mutant
Bos taurus
0.102
-
2-oxoglutarate
at 1 mM Fe2+, wild-type
Bos taurus
0.125
-
2-oxoglutarate
at 1 mM Fe2+, H675E mutant
Bos taurus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
peptide L-aspartate + 2-oxoglutarate + O2
second epidermal growth factor-like domain of bovine protein S
439255
Bos taurus
peptide 3-hydroxy-L-aspartate + succinate + CO2
-
439255
Bos taurus
?
Other publictions for EC 1.14.11.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743376
Tang
Aspartate beta-hydroxylase di ...
Homo sapiens
Oncogenesis
6
e362
2017
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1
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1
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1
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-
-
-
-
-
1
-
-
1
-
-
743379
Ma
Prolyl hydroxylase domain pro ...
Homo sapiens
Oncotarget
8
12983-13002
2017
-
-
-
-
-
-
-
-
-
-
-
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-
2
-
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1
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1
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-
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-
1
3
3
1
-
-
742626
Iwagami
Aspartate beta-hydroxylase mo ...
Homo sapiens
Hepatology
63
1213-1226
2016
-
-
-
-
-
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-
-
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1
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1
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1
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-
-
-
-
-
-
-
2
2
-
-
-
742226
Lyu
-
Research progress of aspartyl ...
Homo sapiens
Cancer Res. Clinic
27
280-282
2015
-
-
-
-
-
-
-
-
1
-
-
-
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1
-
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2
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1
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2
-
-
-
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-
-
-
-
-
-
-
1
2
2
1
-
-
742572
Yang
The aspartyl (asparaginyl) be ...
Homo sapiens
Front. Biosci.
20
902-909
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
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2
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2
-
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-
-
-
-
-
-
-
1
1
1
1
-
-
743130
Borgas
Regulation of aspartyl-(aspar ...
Homo sapiens
J. Nat. Sci.
1
e84
2015
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
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-
-
-
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-
-
-
-
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-
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1
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1
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1
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
743377
Dong
Aspartate beta-hydroxylase ex ...
Homo sapiens
Oncotarget
6
1231-1248
2015
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
743882
Tomimaru
Aspartate-beta-hydroxylase in ...
Homo sapiens
Vaccine
33
1256-1266
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
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-
-
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
742666
Huyan
Inhibition of human natural k ...
Homo sapiens
Int. Immunopharmacol.
23
452-459
2014
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
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-
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2
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2
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-
-
-
-
-
-
-
-
1
1
-
-
-
725118
Yang
Single-chain variable fragment ...
Homo sapiens
Hybridoma
30
69-79
2011
-
-
-
-
-
-
1
-
1
-
-
1
-
3
-
-
-
-
-
2
-
-
1
-
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1
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1
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1
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2
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1
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-
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-
-
-
-
-
-
-
695432
de la Monte
Ethanol inhibition of aspartyl ...
Rattus norvegicus
Alcohol
43
225-240
2009
1
-
1
-
-
-
2
-
-
-
2
-
-
3
-
-
-
-
-
3
-
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1
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1
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2
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2
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-
3
-
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-
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-
-
-
-
-
-
2
2
2
2
-
-
698158
Longato
Overexpression of insulin rece ...
Mus musculus
Hepatology
49
1935-1943
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
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-
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-
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-
-
-
-
-
-
-
1
1
1
1
-
-
698195
Luu
Prognostic value of aspartyl ( ...
Homo sapiens
Hum. Pathol.
40
639-644
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
4
-
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-
1
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
698201
Xue
Monoclonal antibodies against ...
Homo sapiens
Hybridoma
28
251-257
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
-
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1
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1
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1
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1
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-
-
-
-
-
699589
Hardy
Asparaginyl beta-hydroxylation ...
Homo sapiens
J. Mol. Biol.
392
994-1006
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
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1
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1
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1
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1
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1
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1
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
684228
Carter
Ethanol impaired neuronal migr ...
Homo sapiens
Acta Neuropathol.
116
303-315
2008
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
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1
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1
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1
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1
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1
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-
-
-
673951
Gundogan
Role of aspartyl-(asparaginyl) ...
Homo sapiens
Hum. Pathol.
38
50-59
2007
-
-
-
-
-
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2
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4
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4
-
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-
-
-
687022
Yeung
Isolation and characterization ...
Homo sapiens
Hum. Antibodies
16
163-176
2007
-
2
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
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2
1
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687592
Coleman
Asparaginyl hydroxylation of t ...
Homo sapiens, Mus musculus
J. Biol. Chem.
282
24027-24038
2007
-
-
-
2
-
-
2
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2
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2
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2
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673915
Cantarini
Aspartyl-asparagyl beta hydrox ...
Homo sapiens
Hepatology
44
446-457
2006
-
-
-
-
-
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2
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1
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1
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1
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1
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-
-
-
675183
de la Monte
Aspartyl-(asparaginyl)-beta-hy ...
Homo sapiens
J. Hepatol.
44
971-983
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
3
-
-
1
-
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1
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1
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3
-
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1
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-
-
-
-
-
-
675861
Xian
Expression of aspartyl beta-hy ...
Homo sapiens
Mod. Pathol.
19
280-286
2006
-
-
-
-
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2
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2
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1
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2
-
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1
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676817
Cockman
Posttranslational hydroxylatio ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
14767-14772
2006
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657901
Lancaster
Factor inhibiting hypoxia-indu ...
Homo sapiens
Biochem. Soc. Trans.
32
943-945
2004
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
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1
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-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659316
Linke
Substrate requirements of the ...
Homo sapiens
J. Biol. Chem.
279
14391-14397
2004
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
1
-
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3
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659445
Koivunen
Catalytic properties of the as ...
Homo sapiens
J. Biol. Chem.
279
9899-9904
2004
1
-
1
-
-
-
1
3
-
1
-
1
-
1
-
-
1
-
-
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1
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1
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1
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1
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-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439251
Dinchuk
Aspartyl beta-hydroxylase (Asp ...
Mus musculus
J. Biol. Chem.
275
39543-39554
2000
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
14
-
-
2
-
-
-
-
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-
-
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-
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2
-
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-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439252
Ince
Overexpression of human aspart ...
Homo sapiens
Cancer Res.
60
1261-1266
2000
-
-
1
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
1
-
-
4
-
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439253
McGinnis
The five cysteine residues loc ...
Bos taurus
Biochim. Biophys. Acta
1387
454-456
1998
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439254
Lavaissiere
Overexpression of human aspart ...
Homo sapiens
J. Clin. Invest.
98
1313-1323
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439255
McGinnis
Site-directed mutagenesis of r ...
Bos taurus
Biochemistry
35
3957-3962
1996
-
-
1
-
11
-
-
6
-
1
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
11
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439256
Jia
A fully active catalytic domai ...
Bos taurus
Proc. Natl. Acad. Sci. USA
91
7227-7231
1994
-
-
1
-
-
-
1
2
-
1
2
-
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
1
2
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439257
Korioth
Cloning and characterization o ...
Homo sapiens
Gene
150
395-399
1994
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439258
Wang
Bovine liver aspartyl beta-hyd ...
Bos taurus
J. Biol. Chem.
266
14004-14010
1991
-
-
-
-
-
-
-
8
-
1
2
-
-
2
-
-
1
-
-
2
1
1
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
2
-
-
-
-
1
-
2
1
1
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
439259
Gronke
Partial purification and chara ...
Bos taurus
J. Biol. Chem.
265
8558-8565
1990
-
-
-
-
-
-
-
2
1
1
-
-
-
3
-
-
1
-
-
2
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
1
-
2
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
439260
Gronke
Aspartyl beta-hydroxylase: in ...
Mus musculus, Rattus norvegicus
Proc. Natl. Acad. Sci. USA
86
3609-3613
1989
-
-
-
-
-
-
1
-
2
2
-
-
-
5
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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2
2
-
-
-
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-
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-
2
-
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4
-
-
-
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-
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-
-