BRENDA - Enzyme Database show
show all sequences of 1.13.12.6

Long-term ex vivo and in vivo monitoring of tumor progression by using dual luciferases

Morita, N.; Haga, S.; Ohmiya, Y.; Ozaki, M.; Anal. Biochem. 497, 24-26 (2016)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice. Continuous non-invasive measurement of CLuc activity for tumor growth allows us to determine a suitable time to assess tumor spatial distribution and growth, thereby, reducing the animal stress and the experimental cost
Cypridina noctiluca
Engineering
Amino acid exchange
Commentary
Organism
additional information
concept of tumor monitoring using dual luciferases, construction of the expression vector, and evaluation of tumor monitoring systems using dual luciferases from Cypridina noctiluca and firefly Photinus pyralis, overview. The enzymes are expressed in human breast cancercells MDA-MB-231, followed by inoculatin of the MDA-MB-231/FIC cell suspension subcutaneously into the back of 6-week-old male nude mice lacking T-cell function (BALB/cAJcl-nu/nu). The expressed CLuc is secreted into the blood from the cells and circulates in the living body. The blood containing CLuc can be drawn by using glass micro-hematocrit capillary tubes or a syringe
Cypridina noctiluca
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
the enzyme is secreted
Cypridina noctiluca
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cypridina noctiluca
Q75R40
-
-
Application (protein specific)
Application
Commentary
Organism
analysis
the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice. Continuous non-invasive measurement of CLuc activity for tumor growth allows us to determine a suitable time to assess tumor spatial distribution and growth, thereby, reducing the animal stress and the experimental cost
Cypridina noctiluca
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
concept of tumor monitoring using dual luciferases, construction of the expression vector, and evaluation of tumor monitoring systems using dual luciferases from Cypridina noctiluca and firefly Photinus pyralis, overview. The enzymes are expressed in human breast cancercells MDA-MB-231, followed by inoculatin of the MDA-MB-231/FIC cell suspension subcutaneously into the back of 6-week-old male nude mice lacking T-cell function (BALB/cAJcl-nu/nu). The expressed CLuc is secreted into the blood from the cells and circulates in the living body. The blood containing CLuc can be drawn by using glass micro-hematocrit capillary tubes or a syringe
Cypridina noctiluca
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
the enzyme is secreted
Cypridina noctiluca
-
-
Other publictions for EC 1.13.12.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745977
Yasuno
Effects of N-glycosylation de ...
Cypridina noctiluca
Photochem. Photobiol.
94
338-342
2018
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746376
Mitani
Efficient production of glyco ...
Cypridina noctiluca
Protein Expr. Purif.
133
102-109
2017
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744013
Morita
Long-term ex vivo and in vivo ...
Cypridina noctiluca
Anal. Biochem.
497
24-26
2016
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744465
Chase
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Activity and inhibition of Cy ...
Cypridina sp.
Biolumin. Progr.
2015
115-136
2015
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744466
Tsuji
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Chemistry of the luciferases ...
Apogon ellioti, Vargula hilgendorfii
Biolumin. Progr.
2015
137-149
2015
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728578
Tzertzinis
Substrate cooperativity in mar ...
Cypridina noctiluca
PLoS ONE
7
e40099
2012
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Sensitive and convenient yeast ...
Cypridina noctiluca
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82
5768-5776
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699954
Wu
A bioluminescent enzyme immuno ...
Cypridina noctiluca
Luminescence
24
131-133
2009
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Michelini
Luminescent probes and visuali ...
Cypridina noctiluca
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700998
Wu
In vivo far-red luminescence i ...
Cypridina noctiluca
Proc. Natl. Acad. Sci. USA
106
15599-15603
2009
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696008
Inouye
Soluble protein expression in ...
Cypridina noctiluca
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376
448-453
2008
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671321
Wu
Preparation of biotinylated Cy ...
Cypridina noctiluca
Anal. Chem.
79
1634-1638
2007
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684849
Kanjou
Yeast mutant with efficient se ...
Cypridina noctiluca
Biochem. Biophys. Res. Commun.
358
429-434
2007
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685701
Wu
Dual-reporter assay using two ...
Cypridina noctiluca
Biotechniques
42
290,292
2007
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671303
Yamagishi
Perfusion-culture-based secret ...
Cypridina noctiluca
Anal. Biochem.
354
15-21
2006
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677182
Wu
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Syntheses and evaluation of th ...
Vargula hilgendorfii
Tetrahedron Lett.
47
753-756
2006
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Monitoring for dynamic biologi ...
Vargula hilgendorfii
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2004
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655065
Nakajima
cDNA cloning and characterizat ...
Cypridina noctiluca
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68
565-570
2004
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439332
Inouye
Fusions to imidazopyrazinone-t ...
Vargula hilgendorfii
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2000
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439331
Maeda
Engineering of functional chim ...
Vargula hilgendorfii
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147-152
1997
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439329
Toya
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Structure of cypridina bilucef ...
Vargula hilgendorfii
Tetrahedron Lett.
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239-242
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439324
Tsuji
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Cypridina luciferin and lucife ...
Vargula hilgendorfii
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364-372
1978
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439328
Tsuji
Mechanism of the enzyme-cataly ...
Vargula hilgendorfii
Biochem. Biophys. Res. Commun.
74
606-613
1977
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439325
Tsuji
Some properties of luciferase ...
Vargula hilgendorfii
Biochemistry
13
5204-5209
1974
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4
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439330
Shimomura
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Cypridina bioluminescence: lig ...
Vargula hilgendorfii
Science
13
1299-1300
1969
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439327
Stone
The enzyme catalyzed oxidation ...
Cypridina sp.
Biochem. Biophys. Res. Commun.
31
386-391
1968
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439326
Cormier
Evidence for the identity of t ...
Porichthys porosissimus
Biochem. Biophys. Res. Commun.
29
747-752
1967
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