BRENDA - Enzyme Database
show all sequences of 1.13.11.64

Biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. strain JS329

Qu, Y.; Spain, J.C.; Appl. Environ. Microbiol. 76, 1417-1422 (2010)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
1 mM, 98% loss of activity
Bradyrhizobium sp.
o-phenanthroline
1 mM, complete loss of activity
Bradyrhizobium sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions
Bradyrhizobium sp.
Organism
Organism
UniProt
Commentary
Textmining
Bradyrhizobium sp.
D3WZ86
-
-
Renatured (Commentary)
Renatured (Commentary)
Organism
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions
Bradyrhizobium sp.
Storage Stability
Storage Stability
Organism
-20°C, 40% to 50% loss of original activity after 1 week of storage
Bradyrhizobium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitro-6-oxohepta-2,4-dienedioate
-
701822
Bradyrhizobium sp.
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
2nd step of reaction, spontaneous
-
-
?
5-nitrosalicylate + O2
oxidation catalyzed by cell extracts requires 0.97 mol O2/mol 5-nitrosalicylate
701822
Bradyrhizobium sp.
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
overall reaction
-
-
?
5-nitrosalicylate + O2
-
701822
Bradyrhizobium sp.
4-nitro-6-oxohepta-2,4-dienedioate
1st step of reaction
-
-
?
salicylate + O2
-
701822
Bradyrhizobium sp.
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
5NSA dioxygenase
-
Bradyrhizobium sp.
naaB
-
Bradyrhizobium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no requirement of NADPH
Bradyrhizobium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no requirement of NADPH
Bradyrhizobium sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
1 mM, 98% loss of activity
Bradyrhizobium sp.
o-phenanthroline
1 mM, complete loss of activity
Bradyrhizobium sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions
Bradyrhizobium sp.
Renatured (Commentary) (protein specific)
Commentary
Organism
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions
Bradyrhizobium sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 40% to 50% loss of original activity after 1 week of storage
Bradyrhizobium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitro-6-oxohepta-2,4-dienedioate
-
701822
Bradyrhizobium sp.
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
2nd step of reaction, spontaneous
-
-
?
5-nitrosalicylate + O2
oxidation catalyzed by cell extracts requires 0.97 mol O2/mol 5-nitrosalicylate
701822
Bradyrhizobium sp.
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
overall reaction
-
-
?
5-nitrosalicylate + O2
-
701822
Bradyrhizobium sp.
4-nitro-6-oxohepta-2,4-dienedioate
1st step of reaction
-
-
?
salicylate + O2
-
701822
Bradyrhizobium sp.
?
-
-
-
?
Other publictions for EC 1.13.11.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719725
Qu
Molecular and biochemical char ...
Bradyrhizobium sp.
J. Bacteriol.
193
3057-3063
2011
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701822
Qu
Biodegradation of 5-nitroanthr ...
Bradyrhizobium sp.
Appl. Environ. Microbiol.
76
1417-1422
2010
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1
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