Information on EC 1.13.11.64 - 5-nitrosalicylate dioxygenase

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The expected taxonomic range for this enzyme is: Bradyrhizobium

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EC NUMBER
COMMENTARY hide
1.13.11.64
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RECOMMENDED NAME
GeneOntology No.
5-nitrosalicylate dioxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-nitro-6-oxohepta-2,4-dienedioate = 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
show the reaction diagram
spontaneous
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-
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5-nitrosalicylate + O2 = 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
show the reaction diagram
overall reaction
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-
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5-nitrosalicylate + O2 = 4-nitro-6-oxohepta-2,4-dienedioate
show the reaction diagram
(1a)
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-
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SYSTEMATIC NAME
IUBMB Comments
5-nitrosalicylate:oxygen 1,2-oxidoreductase (decyclizing)
The enzyme, characterized from the soil bacterium Bradyrhizobium sp. JS329, is involved in the pathway of 5-nitroanthranilate degradation. It is unusual in being able to catalyse the ring fission without the requirement for prior removal of the nitro group. The product undergoes spontaneous lactonization, with concurrent elimination of the nitro group.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitro-6-oxohepta-2,4-dienedioate
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
show the reaction diagram
5-chlorosalicylate + O2
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + chloride
show the reaction diagram
5-nitrosalicylate + O2
2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite
show the reaction diagram
5-nitrosalicylate + O2
4-nitro-6-oxohepta-2,4-dienedioate
show the reaction diagram
salicylate + O2
?
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no requirement of NADPH
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
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1 mM, 98% loss of activity
o-phenanthroline
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1 mM, complete loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40% to 50% loss of original activity after 1 week of storage
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions
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LINKS TO OTHER DATABASES (specific for EC-Number 1.13.11.64)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)