BRENDA - Enzyme Database show
show all sequences of 1.13.11.32

Kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase

Francis, K.; Gadda, G.; Bioorg. Chem. 37, 167-172 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Butanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
Hexanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KBr
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KBr
-
Neurospora crassa
KCl
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KCl
-
Neurospora crassa
KF
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KF
-
Neurospora crassa
KI
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KI
-
Neurospora crassa
NaNO2
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
NaNO2
competitive inhibitor with respect to ethylnitronate as substrate
Neurospora crassa
NaNO3
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
NaNO3
-
Neurospora crassa
pentanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
propanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters of reductive half reaction
Cyberlindnera mrakii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cyberlindnera mrakii
-
-
-
Neurospora crassa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 butyl-1-nitronate + O2
-
696605
Cyberlindnera mrakii
2 butanal + 2 nitrite
-
-
-
?
2 ethylnitronate + O2
-
696605
Cyberlindnera mrakii
2 ethanal + 2 nitrite
-
-
-
?
2 propyl-1-nitronate + O2
-
696605
Cyberlindnera mrakii
2 propanal + 2 nitrite
-
-
-
?
ethyl nitronate + O2
-
696605
Neurospora crassa
ethanal + nitrite
-
-
-
?
additional information
kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase
696605
Neurospora crassa
?
-
-
-
-
additional information
kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase
696605
Cyberlindnera mrakii
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters of reductive half reaction
Cyberlindnera mrakii
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
binding of sodium nitrite to the enzyme enzymes induces spectral changes in the FMN cofactor bound at the active site of the enzyme
Cyberlindnera mrakii
FMN
binding of sodium nitrite to the enzyme enzymes induces spectral changes in the FMN cofactor bound at the active site of the enzyme
Neurospora crassa
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.95
-
KI
pH 7.4, 30C
Neurospora crassa
1.7
-
NaNO3
pH 7.4, 30C
Neurospora crassa
1.9
-
NaNO3
pH 7.4, 30C
Neurospora crassa
4
-
KBr
pH 7.4, 30C
Neurospora crassa
6
-
KI
pH 7.4, 30C
Cyberlindnera mrakii
9.8
-
NaNO2
pH 7.4, 30C
Cyberlindnera mrakii
11.3
-
KCl
pH 7.4, 30C
Neurospora crassa
12
-
NaNO3
pH 7.4, 30C
Cyberlindnera mrakii
20
-
KBr
pH 7.4, 30C
Cyberlindnera mrakii
30
-
KCl
pH 7.4, 30C
Cyberlindnera mrakii
58
-
pentanal
pH 7.4, 30C
Cyberlindnera mrakii
58
-
propanal
pH 7.4, 30C
Cyberlindnera mrakii
60
-
Butanal
pH 7.4, 30C
Cyberlindnera mrakii
60
-
Hexanal
pH 7.4, 30C
Cyberlindnera mrakii
77
-
KF
pH 7.4, 30C
Neurospora crassa
125
-
KF
pH 7.4, 30C
Cyberlindnera mrakii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
binding of sodium nitrite to the enzyme enzymes induces spectral changes in the FMN cofactor bound at the active site of the enzyme
Cyberlindnera mrakii
FMN
binding of sodium nitrite to the enzyme enzymes induces spectral changes in the FMN cofactor bound at the active site of the enzyme
Neurospora crassa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Butanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
Hexanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KBr
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KBr
-
Neurospora crassa
KCl
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KCl
-
Neurospora crassa
KF
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KF
-
Neurospora crassa
KI
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
KI
-
Neurospora crassa
NaNO2
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
NaNO2
competitive inhibitor with respect to ethylnitronate as substrate
Neurospora crassa
NaNO3
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
NaNO3
-
Neurospora crassa
pentanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
propanal
competitive inhibitor with respect to ethylnitronate as substrate
Cyberlindnera mrakii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.95
-
KI
pH 7.4, 30C
Neurospora crassa
1.7
-
NaNO3
pH 7.4, 30C
Neurospora crassa
1.9
-
NaNO3
pH 7.4, 30C
Neurospora crassa
4
-
KBr
pH 7.4, 30C
Neurospora crassa
6
-
KI
pH 7.4, 30C
Cyberlindnera mrakii
9.8
-
NaNO2
pH 7.4, 30C
Cyberlindnera mrakii
11.3
-
KCl
pH 7.4, 30C
Neurospora crassa
12
-
NaNO3
pH 7.4, 30C
Cyberlindnera mrakii
20
-
KBr
pH 7.4, 30C
Cyberlindnera mrakii
30
-
KCl
pH 7.4, 30C
Cyberlindnera mrakii
58
-
pentanal
pH 7.4, 30C
Cyberlindnera mrakii
58
-
propanal
pH 7.4, 30C
Cyberlindnera mrakii
60
-
Butanal
pH 7.4, 30C
Cyberlindnera mrakii
60
-
Hexanal
pH 7.4, 30C
Cyberlindnera mrakii
77
-
KF
pH 7.4, 30C
Neurospora crassa
125
-
KF
pH 7.4, 30C
Cyberlindnera mrakii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters of reductive half reaction
Cyberlindnera mrakii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 butyl-1-nitronate + O2
-
696605
Cyberlindnera mrakii
2 butanal + 2 nitrite
-
-
-
?
2 ethylnitronate + O2
-
696605
Cyberlindnera mrakii
2 ethanal + 2 nitrite
-
-
-
?
2 propyl-1-nitronate + O2
-
696605
Cyberlindnera mrakii
2 propanal + 2 nitrite
-
-
-
?
ethyl nitronate + O2
-
696605
Neurospora crassa
ethanal + nitrite
-
-
-
?
additional information
kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase
696605
Neurospora crassa
?
-
-
-
-
additional information
kinetic evidence for an anion binding pocket in the active site of nitronate monooxygenase
696605
Cyberlindnera mrakii
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters of reductive half reaction
Cyberlindnera mrakii
Other publictions for EC 1.13.11.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera mrakii, Neurospora crassa, Pseudomonas aeruginosa
Arch. Biochem. Biophys.
493
53-61
2009
-
-
2
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696605
Francis
Kinetic evidence for an anion ...
Cyberlindnera mrakii, Neurospora crassa
Bioorg. Chem.
37
167-172
2009
-
-
-
-
-
-
16
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
2
16
-
-
-
-
-
2
-
-
-
-
16
16
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-