Information on EC 1.13.11.32 - 2-nitropropane dioxygenase

While the enzyme from the fungus Neurospora crassa contains non-covalently bound FMN as the cofactor, that from the yeast Williopsis mrakii contains FAD . This enzyme differs from EC 1.7.3.1, nitroalkane oxidase, in that the preferred substrates are anionic nitronates rather than neutral nitroalkanes [2,3]. The enzyme has broad substrate specificity that is independent of substrate size [2,3]. Some other nitroalkanes, including nitroethane, 1-nitropropane and 3-nitropentan-2-ol, can act as donors, but more slowly.