Application | Comment | Organism |
---|---|---|
environmental protection | laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents | Klebsiella pneumoniae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.24 | - |
2,6-dimethoxyphenol | pH 8.0, 70°C | Klebsiella pneumoniae | |
5.33 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 35°C | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 15% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM | Klebsiella pneumoniae | |
Ca2+ | about 15% loss of maximal activity at 10 mM, about 25% loss of activity at 50 mM | Klebsiella pneumoniae | |
Cu2+ | multicopper enzyme | Klebsiella pneumoniae | |
Mg2+ | about 10% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM | Klebsiella pneumoniae | |
Mn2+ | about 10% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae 601 MCO | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Klebsiella pneumoniae | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Klebsiella pneumoniae 601 MCO | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Klebsiella pneumoniae | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Klebsiella pneumoniae 601 MCO | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55600, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Klebsiella pneumoniae |
70 | - |
substrate: 2,6-dimethoxyphenol | Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | 30°C: about 50% of maximal activity, 40°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Klebsiella pneumoniae |
45 | 80 | 45°C: about 60% of maximal activity, 80°C: about 65% of maximal activity, substrate: 2,6-dimethoxyphenol | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 35°C | Klebsiella pneumoniae | |
2.93 | - |
2,6-dimethoxyphenol | pH 8.0, 70°C | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Klebsiella pneumoniae |
8 | - |
substrate: 2,6-dimethoxyphenol | Klebsiella pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | pH 3.5: about 55% of maximal activity, pH 4.5: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Klebsiella pneumoniae |
7.5 | 8.5 | pH 7.5: about 45% of maximal activity, pH 8.5: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Klebsiella pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 35°C | Klebsiella pneumoniae | |
2.36 | - |
2,6-dimethoxyphenol | pH 8.0, 70°C | Klebsiella pneumoniae |