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Information on EC 1.10.3.2 - laccase
for references in articles please use BRENDA:EC1.10.3.2
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EC Tree 1 Oxidoreductases
1.10 Acting on diphenols and related substances as donors
1.10.3 With oxygen as acceptor
1.10.3.2 laccase
IUBMB Comments
A group of multi-copper proteins of low specificity acting on both o- and p-quinols, and often acting also on aminophenols and phenylenediamine. The semiquinone may react further either enzymically or non-enzymically.
The enzyme appears in viruses and cellular organisms
- 1.10.3.2
- trametes
-
decolor
- versicolor
- abts
-
white-rot
- manganese
-
basidiomycete
- pleurotus
- oxidases
-
wastewater
-
textile
-
effluent
- ostreatus
-
bioremediation
-
electrode
-
lignocellulosic
-
recalcitrant
- pulp
- guaiacol
- straw
- wood
- tyrosinase
- mushroom
-
solid-state
-
submerged
- cellulase
- xylanase
-
brilliant
- phanerochaete
-
chemoradiation
- chrysosporium
- malachite
-
trinuclear
-
veratryl
-
kraft
-
chemoradiotherapy
-
copper-containing
-
reusability
- monophenols
- indigo
- edodes
-
humic
- bagasse
-
brachytherapy
-
bioelectrocatalytic
-
copper-binding
- azoreductase
- monolignols
- industry
-
para-aortic
- food industry
- degradation
- biofuel production
- energy production
- medicine
- environmental protection
- biotechnology
- analysis
- cmcase
- synthesis
showSynonyms
laccase, lacc, phenol oxidase, laccase a, cota-laccase, lac i, diphenol oxidase, poxa1b, laccase2, cota laccase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
benzenediol oxygen oxidoreductase
benzenediol-oxygen oxidoreductase
benzenediol: oxygen oxidoreductase
benzenediol:O2 oxidoreductase
Benzenediol:oxygen oxidoreductase
CotA
CotA-laccase
CotA-type laccase
CueO
Diphenol oxidase
-
-
-
-
FpLcc1
FpLcc2
GMET_RS10855
Hvo_B0205
LAC
Lac-3.5
Lac-4.8
LAC1
Lac2
LAC3
LacA
Lacc
laccase
laccase 1
laccase 3
laccase A
Laccase allele OR
-
-
-
-
Laccase allele TS
-
-
-
-
lacTT
Lcc
lcc1
Lcc2
Lcc9
LccA
Ligninolytic phenoloxidase
-
-
-
-
McoP
MmPPOA
multicopper oxidase
p-benzenediol:dioxygen oxidoreductase
p-diphenol dioxygen oxidoreductase
p-diphenol oxidase
-
-
-
-
p-diphenol: dioxygen oxidoreductase
p-diphenol:O2 oxidoreductase
p-diphenol:oxygen oxidoreductase
p-diphenol:oxygen-oxidoreductase
PCL
polyphenol oxidase A
POXA1b
POXA1w
POXA2
POXA3a
POXA3b
PpoA
SN4LAC
spore coat A protein
spore coat protein A
two-domain laccase
urishiol oxidase
-
-
-
-
urushiol oxidase
-
-
-
-
yellow laccase
additional information
Benzenediol:oxygen oxidoreductase
-
-
-
-
LAC
Oscillatoria subuliformis BDU100712
-
-
-
laccase
Lyophyllum subglobisporium ECN 100606
-
-
-
Lcc2
Lcc2 is comprised of 5 individual isoforms with pI values ranging from 3.5 to 5.8
additional information
-
laccases are members of the MCO, multicopper oxidase, family of enzymes
additional information
Kitasatospora aureofaciens MTCC 7334
-
the enzyme is a typical blue laccase
-
additional information
-
the enzyme is a polyphenol oxidase belonging to the family of multi-copper oxidases
additional information
-
the enzyme is a polyphenol oxidase belonging to the family of multi-copper oxidases
-
additional information
-
the enzyme belongs to the multicopper oxidase family
additional information
Trametes hirsuta Ig-9 / CGMCC 2422
-
the enzyme belongs to the multicopper oxidase family
-
additional information
-
the enzyme belongs to the family of blue oxidases
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
discussion of mechanism
-
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
discussion of mechanism
-
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
nonenzymatic disproportionation of 2 semiquinones to quinone and quinol
-
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
two site ping-pong bi-bi mechanism
-
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
reaction mechanism of the reduction of dioxygen to water, second-order rate constant for the reaction with dioxygen, ping-pong mechanism, kinetic model
-
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
catalytic reaction mechanism, modelling, overview
-
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
gossypol biosynthesis, justicidin B biosynthesis, matairesinol biosynthesis, sesamin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
benzenediol:oxygen oxidoreductase
A group of multi-copper proteins of low specificity acting on both o- and p-quinols, and often acting also on aminophenols and phenylenediamine. The semiquinone may react further either enzymically or non-enzymically.
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CAS REGISTRY NUMBER
COMMENTARY
80498-15-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2Z,2'Z)-2,2'-(1Z,2Z)-hydrazine-1,2-diylidenebis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
(4a'S)-2',3',6',7'-tetrahydroxy-1,1'',3,3''-tetramethyl-4a',9a'-dihydro-2H,2''H-dispiro[pyrimidine-5,9'-anthracene-10',5''-pyrimidine]-2,2'',4,4'',6,6''(1H,1''H,3H,3''H)-hexone + O2
2',3',6',7'-tetrahydroxy-1,1'',3,3''-tetramethyldispiro[pyrimidine-5(2H),9'(10'H)-anthracene-10',5''(2''H)-pyrimidine]-2,2'',4,4'',6,6''-(1H,1''H,3H,3''H)-hexone + H2O2
-
Substrates: -
Products: a polycyclic dispiropyrimidinone
Products: a polycyclic dispiropyrimidinone
?
1,4-dioxane + O2
?
Substrates: the oxidation reaction is accelerated by 25, 22, 6 and 19% in presence of 1 mM syringaldehyde, vanillin, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and guaiacol mediators respectively
Products: -
Products: -
?
1-(4-hydroxy-3,5-dimethoxyphenyl)ethanone + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2-methylcatechol + O2
?
-
Substrates: 17.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
3,5-dimethoxy-hydroxy-benzaldazine + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
3-(4-dimethylamino-1-phenylazo) benzene sulfonic acid sodium salt + O2
?
-
Substrates: i.e. methyl orange, decolorization of the azo dye
Products: -
Products: -
?
3-dimethylaminobenzoic acid + 3-methyl-2-benzothiazolinone hydrazone + O2
? + H2O
-
Substrates: ratio of substrates 1:0.07
Products: -
Products: -
?
4 4-hydroxy-3,5-dimethoxy-benzaldehyde azine + O2
?
-
Substrates: -
Products: -
Products: -
?
4 N,N-dimethyl-1,4-phenylenediamine + O2
?
-
Substrates: -
Products: -
Products: -
?
4-aminoantipyrine + O2
? + H2O
-
Substrates: 19% of the activity with N,N-dimethyl-p-phenylenediamine sulfate
Products: -
Products: -
?
4-hydroxy-1,6-dimethylpyridin-2(1H)-one + 3-methoxy-1,2-benzenediol
7,8-dihydroxy-6-methoxy-2,3-dimethylbenzofuro[3,2-c]pyridin-1(2H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-1,6-dimethylpyridin-2(1H)-one + 3-methyl-1,2-benzenediol
7,8-dihydroxy-2,3,6-trimethyl[1]benzofuro[3,2-c]pyridin-1(2H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-1,6-dimethylpyridin-2(1H)-one + benzenediol
7,8-dihydroxy-2,3-dimethyl[1]benzofuro[3,2-c]pyridin-1(2H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-1-thiocoumarin + 3-methoxy-1,2-benzenediol
8,9-dihydroxy-10-methoxy-6H-thiochromeno[4,3-b][1]benzofuran-6-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-1-thiocoumarin + 3-methyl-1,2-benzenediol
8,9-dihydroxy-10-methyl-6H-thiochromeno[4,3-b][1]benzofuran-6-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-1-thiocoumarin + benzenediol
8,9-dihydroxy-6H-thiochromeno[4,3-b][1]benzofuran-6-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-6-methylpyridin-2(1H)-one + 3-methoxy-1,2-benzenediol
7,8-dihydroxy-6-methoxy-3-methyl[1]benzofuro[3,2-c]pyridin-1(2H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-6-methylpyridin-2(1H)-one + 3-methyl-1,2-benzenediol
7,8-dihydroxy-3,6-dimethyl[1]benzofuro[3,2-c]pyridin-1(2H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-6-methylpyridin-2(1H)-one + benzenediol
7,8-dihydroxy-3-methyl[1]benzofuro[3,2-c]pyridin-1(2H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxyphenylacetic acid + O2
?
-
Substrates: second-order rate constant for the reaction with dioxygen
Products: -
Products: -
?
4-hydroxyquinolin-2(1H)-one + 3-methoxy-1,2-benzenediol
8,9-dihydroxy-10-methoxy[1]benzofuro[3,2-c]quinolin-6(5H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxyquinolin-2(1H)-one + 3-methyl-1,2-benzenediol
8,9-dihydroxy-10-methyl[1]benzofuro[3,2-c]quinolin-6(5H)-one
-
Substrates: -
Products: -
Products: -
?
4-hydroxyquinolin-2(1H)-one + benzenediol
8,9-dihydroxy[1]benzofuro[3,2-c]quinolin-6(5H)-one
-
Substrates: -
Products: -
Products: -
?
4-morpholinoaniline + 2,5-dihydroxybenzoic acid ethyl ester + O2
2,5-bis-(4-morpholin-4-yl-phenylamino)-3,6-dioxocyclohexa-1,4-dienecarboxylic acid ethyl ester
-
Substrates: -
Products: -
Products: -
?
4-morpholinoaniline + 2,5-dihydroxybenzoic acid methyl ester + O2
2,5-bis-(4-morpholin-4-yl-phenylamino)-3,6-dioxocyclohexa-1,4-dienecarboxylic acid methyl ester
-
Substrates: -
Products: -
Products: -
?
acid red 6B + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of acid red 6B is 98.6%
Products: -
Products: -
?
alpha-naphthol + O2
? + H2O
-
Substrates: 11.7% of activity with syringaldazine
Products: -
Products: -
?
benzenediol + thiobarbituric acid
1,1'',3,3''-tetraethyl-2',3',6',7'-tetrahydroxy-2,2''-dithioxo-2H,2''H-dispiro[pyrimidine-5,9'-anthracene-10',5''-pyrimidine]-4,4'',6,6''(1H,1''H,3H,3''H)-tetrone
-
Substrates: -
Products: -
Products: -
?
benzidine + O2
?
-
Substrates: 22.53% of the activity with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
beta-(3,4-dihydroxyphenyl)alanine + O2
beta-(3,4-dihydroxyphenyl)alanine quinone + H2O
-
Substrates: i.e. L-dopa
Products: -
Products: -
?
chlorogenic acid + O2
? + H2O
-
Substrates: 26.5% of the activity with N,N-dimethyl-p-phenylenediamine sulfate
Products: -
Products: -
?
Crystal violet + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Diamond Black PV 200 + ?
2-amino-1-phenol-4-sulfonic acid + 1,5-naphthalenediol + ?
-
Substrates: i.e. Mordant Black 9, Eriochrome Black PV, wool azo dye
Products: -
Products: -
?
dihydroxyphenylalanine + O2
? + H2O
-
Substrates: 23.6% of the activity with N,N-dimethyl-p-phenylenediamine sulfate
Products: -
Products: -
?
Evans Blue + O2
?
-
Substrates: activity with mutant enzyme V159E/N398D
Products: -
Products: -
?
Gly-Leu-Tyr + O2
?
-
Substrates: 21% of the activity with caffeic acid
Products: -
Products: -
?
L-3,4-dihydroxyphenylalanine + O2
?
-
Substrates: i.e. L-dopa
Products: -
Products: -
?
L-Tyr + O2
?
-
Substrates: 11% of the activity with caffeic acid
Products: -
Products: -
?
lignin sulfonic acid + O2
? + H2O
-
Substrates: 0.9% of activity with syringaldazine
Products: -
Products: -
?
m-cresol + O2
6-hydroxy-3-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
m-methoxyphenol + O2
? + H2O
-
Substrates: 15% of the activity with N,N-dimethyl-p-phenylenediamine sulfate
Products: -
Products: -
?
morpholine + 1,4-dihydroxynaphthalene + O2
2-morpholin-4-yl-[1,4]naphthoquinone
-
Substrates: -
Products: -
Products: -
?
morpholine + 1,4-hydroquinone + O2
2,5-dimorpholin-4-yl-[1,4]benzoquinone
-
Substrates: -
Products: -
Products: -
?
morpholine + 2,3-dimethyl-1,4-hydroquinone + O2
2,3-dimethyl-5-morpholin-4-yl-[1,4]benzoquinone
-
Substrates: -
Products: -
Products: -
?
morpholine + 2,5-dihydroxy-N-(2-hydroxyethyl)-benzamide + O2
2-hydroxy-5-morpholin-4-yl-3,6-dioxocyclohexa-1,4-dienecarboxylic acid (2-hydroxyethyl)-amide
-
Substrates: -
Products: -
Products: -
?
morpholine + 2-methyl-1,4-hydroquinone + O2
2-methyl-6-morpholin-4-yl-[1,4]benzoquinone + 2-methyl-5-morpholin-4-yl-[1,4]benzoquinone
-
Substrates: -
Products: -
Products: -
?
N,N-dimethyl-4-phenylenediamine + O2
?
-
Substrates: -
Products: -
Products: -
?
N,N-dimethyl-p-phenylenediamine sulfate + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
N,N-dimethylbarbituric acid + catechol
2',3',6',7'-tetrahydroxy-1,1'',3,3''-tetramethyldispiro[pyrimidine-5(2H),9'(10'H)-anthracene-10',5''(2''H)-pyrimidine]-2,2'',4,4'',6,6''-(1H,1''H,3H,3''H)-hexone
-
Substrates: -
Products: a polycyclic dispiropyrimidinone
Products: a polycyclic dispiropyrimidinone
?
p-coumaric acid + O2
?
-
Substrates: 79% of the activity with caffeic acid
Products: -
Products: -
?
p-cresol + O2
? + H2O
-
Substrates: 6.35% of activity with syringaldazine
Products: -
Products: -
?
p-phenylenediamine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 563% of the activity with phenol
Products: -
Products: -
?
propyl gallate + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 94% of the activity with phenol
Products: -
Products: -
?
Reactive blue 19 + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Reactive blue 5 + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
reactive brilliant orange K-7R + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive 203 brilliant orange K-7R is 94.9%
Products: -
Products: -
?
Remazol Brilliant Blue + O2
?
-
Substrates: greater efficiency in Remazol Brilliant Blue decolourization (90%) in absence of redox mediator
Products: -
Products: -
?
syringic acid + O2
2,6-dimethoxy-1,4-benzoquinone + CO2 + H2O
-
Substrates: -
Products: -
Products: -
?
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
?
-
Substrates: -
Products: -
Products: -
?
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
?
Substrates: -
Products: -
Products: -
?
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
?
Substrates: -
Products: -
Products: -
?
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
?
Substrates: -
Products: -
Products: -
?
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2
?
-
Substrates: -
Products: -
Products: -
?
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid + O2
?
-
Substrates: -
Products: -
Products: -
?
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid + O2
?
-
Substrates: -
Products: -
Products: -
?
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + O2
?
-
Substrates: -
Products: -
Products: -
?
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + O2
?
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2 catechol + O2
2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate) + O2
?
Obba rivulosa T241i
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
Collybia nuda ECN 100605
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
Cortinarius sp. 2 ECN 100602
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
Inocybe longicystis
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
Leptonia lazunila
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
Lyophyllum subglobisporium
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
Lyophyllum subglobisporium ECN 100606
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Agrocybe pediades AH40210
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Bacillus amyloliquefaciens TCCC 111018
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Bacillus sp. MSK-01
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Deconica castanella CCIBt278
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Sphingobacterium sp. ksn-11
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Streptomyces cyaneus CECT 3335
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Streptomyces griseoflavus Ac-993
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Trametes polyzona WRF03
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
E1ACR6
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid + O2
?
-
Substrates: spectrophotometric assay
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Myrioconium sp.
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Myrioconium sp. UHH 1-13-18-4
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: 100% activity
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Kitasatospora aureofaciens MTCC 7334
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Lentinus brumalis ibrc05015
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
FJ560721
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
FJ560721
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Oscillatoria subuliformis
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Oscillatoria subuliformis BDU100712
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Phoma sp. UHH 5-1-03 / DSM 22425
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Phoma sp. UHH 5-1-03 / DSM 22425
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: best substrate
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: best substrate
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: high activity
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Trametes hirsuta Ig-9 / CGMCC 2422
-
Substrates: high activity
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: 73% of the activity compared to syringaldazine
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: 73% of the activity compared to syringaldazine
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: catalytic efficiency on 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) is 7.5 times of that on 2,6-dimethoxyphenol, 22 times of that on catechol and 65times higher than that on guaiacol
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: catalytic efficiency on 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) is 7.5 times of that on 2,6-dimethoxyphenol, 22 times of that on catechol and 65times higher than that on guaiacol
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Cubamyces flavidus MTCC-145
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Lactiplantibacillus plantarum J16 CECT 8944
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Limnospira platensis CFTRI
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Sporothrix carnis
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Sporothrix carnis CPF-05
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
?
Coriolopsis gallica UAMH 8260
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Fomes sclerodermeus
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Fomes sclerodermeus BAFC 2752
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: 82% of activity with syringaldazine
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: 53% of the activity with phenol
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
Phlebia fascicularia
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: 35% of the activity with phenol
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
Q5I7J0
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
Q5I7J0
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: 44% of the activity with phenol
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
G2H480
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Substrates: i.e. ABTS
Products: -
Products: -
?
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2
?
Substrates: i.e. ABTS
Products: -
Products: -
?
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2
?
-
Substrates: i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation
Products: -
Products: -
?
2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2
?
-
Substrates: i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation
Products: -
Products: -
?
2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2
?
-
Substrates: i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation
Products: -
Products: -
?
2,2,6,6-tetramethyl-piperidine-1-oxyl radical + O2
?
-
Substrates: i.e. TEMPO, model of synergistic TEMPO and activator PSPX oxidation
Products: -
Products: -
?
2,2,6,6-tetramethylpiperidin-N-oxide radical + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2,6,6-tetramethylpiperidin-N-oxide radical + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) + O2
?
-
Substrates: 56% of the activity with 2,6-dimethoxyphenol
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
2,4-dichlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 80% of the activity with phenol
Products: -
Products: -
?
2,4-dichlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 89% of the activity with phenol
Products: -
Products: -
?
2,4-dichlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 93% of the activity with phenol
Products: -
Products: -
?
2,5-xylidine + O2
?
Substrates: the enzyme also transforms the non-phenolic substrate at pH 5.0, native and recombinant enzymes, wild-type and mutant enzymes, overview
Products: -
Products: -
?
2,6-dichlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 49% of the activity with phenol
Products: -
Products: -
?
2,6-dichlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 176% of the activity with phenol
Products: -
Products: -
?
2,6-dichlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 258% of the activity with phenol
Products: -
Products: -
?
2,6-dimethoxyphenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 3840% of the activity with phenol
Products: -
Products: -
?
2,6-dimethoxyphenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 104% of the activity with phenol
Products: -
Products: -
?
2,6-dimethoxyphenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 159% of the activity with phenol
Products: -
Products: -
?
2,6-dimethoxyphenol + H2O
?
-
Substrates: about 5% of the activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
Fomes sclerodermeus
-
Substrates: preferred substrate
Products: i.e. coerulignone
Products: i.e. coerulignone
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
Fomes sclerodermeus BAFC 2752
-
Substrates: preferred substrate
Products: i.e. coerulignone
Products: i.e. coerulignone
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: i.e. cerulignone
Products: i.e. cerulignone
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: i.e. cerulignone
Products: i.e. cerulignone
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
Loweporus lividus
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: preferred substrate
Products: i.e. coerulignone
Products: i.e. coerulignone
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: preferred substrate
Products: i.e. coerulignone
Products: i.e. coerulignone
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 69% of the activity compared to syringaldazine
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 69% of the activity compared to syringaldazine
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 75.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 75.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 7.1% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Lactiplantibacillus plantarum J16 CECT 8944
-
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
FJ560721
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 24% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
Substrates: -
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 96% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
?
-
Substrates: 96% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
? + H2O
-
Substrates: 60% of activity with syringaldazine
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
? + H2O
-
Substrates: 36% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethoxyphenol + O2
? + H2O
-
Substrates: 36% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
2,6-dimethylphenol + O2
?
-
Substrates: 6% of the activity with 2,6-dimethoxyphenol
Products: -
Products: -
?
2,6-dimethylphenol + O2
?
-
Substrates: -
Products: -
Products: -
?
2,6-dimethylphenol + O2
?
-
Substrates: -
Products: -
Products: -
?
2,6-dimethylphenol + O2
?
-
Substrates: 1% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate)
Products: -
Products: -
?
2-methoxyphenol + O2
?
-
Substrates: 8.6% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
3-aminobenzoic acid + O2
?
Trametes hirsuta Ig-9 / CGMCC 2422
-
Substrates: low activity
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: best substrate
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
Substrates: -
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
?
-
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
FJ560721
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
FJ560721
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 syringic acid + O2
4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 syringic acid + O2
4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 syringic acid + O2
4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 syringic acid + O2
4 4-carboxy-2,6-dimethoxyphenoxyl + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4-aminoantipyrine + phenol + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
-
Substrates: i.e. syringaldazine
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
Substrates: i.e. syringaldazine
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
Substrates: i.e. syringaldazine
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
Substrates: i.e. syringaldazine
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
-
Substrates: i.e. syringaldazine
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
-
Substrates: i.e. syringaldazine
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
-
Substrates: -
Products: -
Products: -
?
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2
?
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
4-methylcatechol + O2
?
Sporothrix carnis
-
Substrates: 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
4-methylcatechol + O2
?
Sporothrix carnis CPF-05
-
Substrates: 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
4-methylcatechol + O2
? + H2O
-
Substrates: 0.9% of activity with syringaldazine
Products: -
Products: -
?
4-methylcatechol + O2
? + H2O
-
Substrates: 18% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
4-methylcatechol + O2
? + H2O
-
Substrates: 18% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
Adizol black B + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Adizol black B + O2
?
Bacillus amyloliquefaciens TCCC 111018
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
alpha-naphthol + O2
?
-
Substrates: 22.0% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
Azophloxine + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Azophloxine + O2
?
Bacillus amyloliquefaciens TCCC 111018
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Bromocresol Purple + O2
?
-
Substrates: the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator
Products: -
Products: -
?
Bromocresol Purple + O2
?
-
Substrates: the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator
Products: -
Products: -
?
caffeic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 713% of the activity with phenol
Products: -
Products: -
?
caffeic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 126% of the activity with phenol
Products: -
Products: -
?
caffeic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 39% of the activity with phenol
Products: -
Products: -
?
caffeic acid + O2
?
-
Substrates: 24.3% of the activity with guaiacol
Products: -
Products: -
?
caffeic acid + O2
?
-
Substrates: 24.3% of the activity with guaiacol
Products: -
Products: -
?
catechol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 1340% of the activity with phenol
Products: -
Products: -
?
catechol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 1340% of the activity with phenol
Products: -
Products: -
?
catechol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 136% of the activity with phenol
Products: -
Products: -
?
catechol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 136% of the activity with phenol
Products: -
Products: -
?
catechol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 176% of the activity with phenol
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 85.4% of the activity with guaiacol
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 85.4% of the activity with guaiacol
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 28% of the activity compared to syringaldazine
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 27.5% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 1.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 1.8% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 11.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
?
-
Substrates: 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
catechol + O2
? + H2O
-
Substrates: 11% of activity with syringaldazine
Products: -
Products: -
?
catechol + O2
? + H2O
-
Substrates: 2.44% of activity with syringaldazine
Products: -
Products: -
?
catechol + O2
? + H2O
-
Substrates: 6.2% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
Congo Red + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Congo Red + O2
?
Bacillus amyloliquefaciens TCCC 111018
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Congo Red + O2
?
Substrates: the enzyme decolorizes the azo dye
Products: -
Products: -
?
Congo Red + O2
?
Substrates: the enzyme decolorizes the azo dye
Products: -
Products: -
?
coniferyl alcohol + O2
?
Substrates: the enzyme transforms the non-phenolic substrate at pH 4.5 only when recombinantly expressed in the yeast Yarrowia lipolytica strain Po1t, overview
Products: -
Products: -
?
Coomassie brilliant blue + O2
?
-
Substrates: 72.35% decolourisation efficiency
Products: -
Products: -
?
Coomassie brilliant blue + O2
?
Trametes polyzona WRF03
-
Substrates: 72.35% decolourisation efficiency
Products: -
Products: -
?
diethylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 10% of the activity with phenol
Products: -
Products: -
?
diethylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 60% of the activity with phenol
Products: -
Products: -
?
diethylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 103% of the activity with phenol
Products: -
Products: -
?
dimethylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 40% of the activity with phenol
Products: -
Products: -
?
dimethylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 160% of the activity with phenol
Products: -
Products: -
?
dimethylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 165% of the activity with phenol
Products: -
Products: -
?
DOPA + O2
? + H2O
-
Substrates: the enzyme laccase catalyses the formation of melanin by oxidizing L-DOPA, initiating a series of presumably spontaneous reactions that ultimately leads to the polymerization of the pigment in the yeast cell wall. There, melanin protects the cell from a multitude of environmental and host assaults. Kinetics of dopachrome formation are similar for L- and D-DOPA
Products: -
Products: -
?
Erichrome black + O2
?
-
Substrates: 40.20% decolourisation efficiency
Products: -
Products: -
?
Erichrome black + O2
?
Trametes polyzona WRF03
-
Substrates: 40.20% decolourisation efficiency
Products: -
Products: -
?
ferrocenemonocarboxylic acid + O2
?
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
Coriolisimus fulvocinerea
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
3-(4-hydroxy-3-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
ferulic acid + O2
?
-
Substrates: 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
ferulic acid + O2
?
-
Substrates: 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
ferulic acid + O2
? + H2O
-
Substrates: 13.6% of activity with syringaldazine
Products: -
Products: -
?
ferulic acid + O2
? + H2O
-
Substrates: 9.4% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
gallic acid + O2
?
-
Substrates: 13.7% of the activity with guaiacol
Products: -
Products: -
?
gallic acid + O2
?
-
Substrates: 13.7% of the activity with guaiacol
Products: -
Products: -
?
gallic acid + O2
?
-
Substrates: 45% of the activity compared to syringaldazine
Products: -
Products: -
?
gallic acid + O2
?
-
Substrates: 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 1500% of the activity with phenol
Products: -
Products: -
?
guaiacol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 55% of the activity with phenol
Products: -
Products: -
?
guaiacol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 55% of the activity with phenol
Products: -
Products: -
?
guaiacol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 61% of the activity with phenol
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Coriolisimus fulvocinerea
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: i.e. 1-hydroxy-2-methoxybenzene
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Streptomyces cyaneus CECT 3335
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
Trametes polyzona MPS1-3
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
6-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 90.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 90.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 2.3% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 2.3% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 1.4% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 16% of the activity with 2,6-dimethoxyphenol
Products: -
Products: -
?
guaiacol + O2
?
Sporothrix carnis
-
Substrates: 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
Sporothrix carnis CPF-05
-
Substrates: 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
Substrates: -
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 83% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
?
-
Substrates: 83% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
? + H2O
-
Substrates: 21% of activity with syringaldazine
Products: -
Products: -
?
guaiacol + O2
? + H2O
-
Substrates: 6.41% of activity with syringaldazine
Products: -
Products: -
?
guaiacol + O2
? + H2O
-
Substrates: 33% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
guaiacol + O2
? + H2O
-
Substrates: 33% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
hydrocaffeic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 1200% of the activity with phenol
Products: -
Products: -
?
hydrocaffeic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 190% of the activity with phenol
Products: -
Products: -
?
hydrocaffeic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 185% of the activity with phenol
Products: -
Products: -
?
hydroquinone + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 494% of the activity with phenol
Products: -
Products: -
?
hydroquinone + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 494% of the activity with phenol
Products: -
Products: -
?
hydroquinone + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 63% of the activity with phenol
Products: -
Products: -
?
hydroquinone + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 63% of the activity with phenol
Products: -
Products: -
?
hydroquinone + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 89% of the activity with phenol
Products: -
Products: -
?
hydroquinone + O2
?
-
Substrates: 13.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
hydroquinone + O2
? + H2O
-
Substrates: 9.95% of activity with syringaldazine
Products: -
Products: -
?
hydroquinone + O2
? + H2O
-
Substrates: 60% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
hydroquinone + O2
? + H2O
-
Substrates: 60% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
indigo carmine + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
indigo carmine + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of Indigo carmine is 99.1%
Products: -
Products: -
?
K4[Fe(CN)6] + O2
?
Substrates: -
Products: -
Products: -
?
L-DOPA + O2
?
-
Substrates: 35% of the activity compared to syringaldazine
Products: -
Products: -
?
L-DOPA + O2
?
-
Substrates: 35% of the activity compared to syringaldazine
Products: -
Products: -
?
L-DOPA + O2
?
-
Substrates: 13.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
L-DOPA + O2
? + H2O
-
Substrates: 15.4% of activity with syringaldazine
Products: -
Products: -
?
m-chlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 80% of the activity with phenol
Products: -
Products: -
?
m-chlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 45% of the activity with phenol
Products: -
Products: -
?
m-chlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 50% of the activity with phenol
Products: -
Products: -
?
Malachite green
indigo carmine
Substrates: -
Products: -
Products: -
?
Malachite Green + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Malachite Green + O2
?
-
Substrates: the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator
Products: -
Products: -
?
Malachite Green + O2
?
-
Substrates: the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator
Products: -
Products: -
?
Malachite Green + O2
?
-
Substrates: 57.84% decolourisation efficiency
Products: -
Products: -
?
Malachite Green + O2
?
Trametes polyzona WRF03
-
Substrates: 57.84% decolourisation efficiency
Products: -
Products: -
?
melanin + O2
?
-
Substrates: the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview
Products: -
Products: -
?
melanin + O2
?
-
Substrates: the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview
Products: -
Products: -
?
Methyl orange + O2
?
-
Substrates: 47.55% decolourisation efficiency
Products: -
Products: -
?
Methyl orange + O2
?
Trametes polyzona WRF03
-
Substrates: 47.55% decolourisation efficiency
Products: -
Products: -
?
Methyl Violet + O2
?
-
Substrates: the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator
Products: -
Products: -
?
Methyl Violet + O2
?
-
Substrates: the enzyme efficiently decolorized triphenylmethane dyes such as Malachite Green (87.8%), Bromocresol Purple (71.6%), and Methyl Violet (68.1%) without redox mediator
Products: -
Products: -
?
N,N-dimethyl-1,4-phenylenediamine + O2
?
-
Substrates: 49.4% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
N,N-dimethyl-1,4-phenylenediamine + O2
?
-
Substrates: 42.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
N,N-dimethyl-p-phenylenediamine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 900% of the activity with phenol
Products: -
Products: -
?
N,N-dimethyl-p-phenylenediamine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 278% of the activity with phenol
Products: -
Products: -
?
N,N-dimethyl-p-phenylenediamine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 377% of the activity with phenol
Products: -
Products: -
?
N,N-dimethyl-p-phenylenediamine + O2
?
-
Substrates: -
Products: -
Products: -
?
N,N-dimethyl-p-phenylenediamine + O2
?
-
Substrates: -
Products: -
Products: -
?
N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 15% of the activity with phenol
Products: -
Products: -
?
N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 143% of the activity with phenol
Products: -
Products: -
?
N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 198% of the activity with phenol
Products: -
Products: -
?
o-chlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 190% of the activity with phenol
Products: -
Products: -
?
o-chlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 102% of the activity with phenol
Products: -
Products: -
?
o-chlorophenol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 150% of the activity with phenol
Products: -
Products: -
?
o-cresol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 816% of the activity with phenol
Products: -
Products: -
?
o-cresol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 40% of the activity with phenol
Products: -
Products: -
?
o-cresol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 39% of the activity with phenol
Products: -
Products: -
?
o-cresol + O2
6-hydroxy-2-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
o-cresol + O2
6-hydroxy-2-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
o-dianisidine + O2
?
-
Substrates: 90% of the activity compared to syringaldazine
Products: -
Products: -
?
o-dianisidine + O2
?
-
Substrates: 90% of the activity compared to syringaldazine
Products: -
Products: -
?
o-dianisidine + O2
?
Substrates: -
Products: -
Products: -
?
o-tolidine + O2
?
-
Substrates: best substrate
Products: -
Products: -
?
o-tolidine + O2
?
-
Substrates: 9.0% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
o-toluidine + O2
?
-
Substrates: 42.53% of the activity with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
o-toluidine + O2
?
-
Substrates: 15.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
o-toluidine + O2
?
-
Substrates: 15.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
p-cresol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
p-cresol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
p-cresol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
p-cresol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
p-cresol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
p-cresol + O2
6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
p-hydroxybenzoic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 52% of the activity with phenol
Products: -
Products: -
?
p-hydroxybenzoic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 35% of the activity with phenol
Products: -
Products: -
?
p-hydroxybenzoic acid + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 3% of the activity with phenol
Products: -
Products: -
?
p-phenylenediamine + O2
?
-
Substrates: 107% of the activity compared to syringaldazine
Products: -
Products: -
?
p-phenylenediamine + O2
?
-
Substrates: 46% of the activity with 2,6-dimethoxyphenol
Products: -
Products: -
?
p-phenylenediamine + O2
?
-
Substrates: 7.5% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate)
Products: -
Products: -
?
p-phenylenediamine + O2
? + H2O
Substrates: -
Products: -
Products: -
?
p-phenylenediamine + O2
? + H2O
-
Substrates: 33% of the activity with N,N-dimethyl-p-phenylenediamine sulfate
Products: -
Products: -
?
p-toluidine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 348% of the activity with phenol
Products: -
Products: -
?
p-toluidine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 70% of the activity with phenol
Products: -
Products: -
?
p-toluidine + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 51% of the activity with phenol
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
Oscillatoria subuliformis
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
Oscillatoria subuliformis BDU100712
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
pyrocatechol + O2
?
Sporothrix carnis
-
Substrates: 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
pyrocatechol + O2
?
Sporothrix carnis CPF-05
-
Substrates: 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
pyrogallol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 473% of the activity with phenol
Products: -
Products: -
?
pyrogallol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 8% of the activity with phenol
Products: -
Products: -
?
pyrogallol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 10% of the activity with phenol
Products: -
Products: -
?
pyrogallol + O2
?
-
Substrates: 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
pyrogallol + O2
?
-
Substrates: 95.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
pyrogallol + O2
?
-
Substrates: 3.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
pyrogallol + O2
?
-
Substrates: 57% of the activity with 2,6-dimethoxyphenol
Products: -
Products: -
?
pyrogallol + O2
?
-
Substrates: 7.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
pyrogallol + O2
?
-
Substrates: 19% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate)
Products: -
Products: -
?
pyrogallol + O2
? + H2O
-
Substrates: 7% of activity with syringaldazine
Products: -
Products: -
?
pyrogallol + O2
? + H2O
-
Substrates: 22% of the activity with N,N-dimethyl-p-phenylenediamine sulfate
Products: -
Products: -
?
pyrogallol + O2
? + H2O
-
Substrates: 2% of activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
Reactive Black B + O2
?
Substrates: the enzyme decolorizes the azo dye
Products: -
Products: -
?
Reactive Black B + O2
?
Substrates: the enzyme decolorizes the azo dye
Products: -
Products: -
?
Reactive Black WNN + O2
?
Substrates: the enzyme decolorizes the azo dye
Products: -
Products: -
?
Reactive Black WNN + O2
?
Substrates: the enzyme decolorizes the azo dye
Products: -
Products: -
?
reactive blue M-2GE + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive blue M-2GE is 71.9%
Products: -
Products: -
?
reactive blue M-2GE + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive blue M-2GE is 71.9%
Products: -
Products: -
?
reactive brilliant red KD-8B + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive brilliant red KD-8B is 89.2%
Products: -
Products: -
?
reactive brilliant red KD-8B + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive brilliant red KD-8B is 89.2%
Products: -
Products: -
?
reactive brilliant red KM-8B + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive brilliant red KM-8B is 82.5%
Products: -
Products: -
?
reactive brilliant red KM-8B + O2
?
Substrates: addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive brilliant red KM-8B is 82.5%
Products: -
Products: -
?
Remazol Brilliant Blue R + O2
?
Substrates: decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator
Products: -
Products: -
?
Remazol Brilliant Blue R + O2
?
-
Substrates: the partial purified laccase shows a high efficient decolorization ability of Remazol Brilliant Blue R with 80.8% within 5 h at 30°C and pH 5.5
Products: -
Products: -
?
Remazol Brilliant Blue R + O2
?
-
Substrates: the partial purified laccase shows a high efficient decolorization ability of Remazol Brilliant Blue R with 80.8% within 5 h at 30°C and pH 5.5
Products: -
Products: -
?
Remazol Brilliant Blue R + O2
?
Substrates: the enzyme decolorizes the anthraquinone dye
Products: -
Products: -
?
Remazol Brilliant Blue R + O2
?
Substrates: the enzyme decolorizes the anthraquinone dye
Products: -
Products: -
?
resorcinol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 731% of the activity with phenol
Products: -
Products: -
?
resorcinol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 731% of the activity with phenol
Products: -
Products: -
?
resorcinol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 79% of the activity with phenol
Products: -
Products: -
?
resorcinol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 79% of the activity with phenol
Products: -
Products: -
?
resorcinol + 4-aminoantipyrine + O2
? + H2O
-
Substrates: 73% of the activity with phenol
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
Coriolisimus fulvocinerea
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
sinapic acid + O2
3-(3,5-dimethoxy-4-oxo-cyclohexa-1,5-dienyl)-acrylic acid + H2O
-
Substrates: -
Products: -
Products: -
?
syringaldazine + O2
?
Substrates: 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: -
Products: -
Products: -
?
syringaldazine + O2
?
Substrates: 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: -
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: 0.5% compared to the activity with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
syringaldazine + O2
?
Loweporus lividus
-
Substrates: i.e. 2-hydroxy-3, 5-dimethoxybenaldehyde azine
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: i.e. 4-hydroxy-3,5-dimethoxybenzaldehyde azine
Products: -
Products: -
?
syringaldazine + O2
?
Obba rivulosa T241i
-
Substrates: i.e. 4-hydroxy-3,5-dimethoxybenzaldehyde azine
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: 3% of the activity with 2,6-dimethoxyphenol
Products: -
Products: -
?
syringaldazine + O2
?
Substrates: -
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: 1% of the activity with 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate)
Products: -
Products: -
?
syringaldazine + O2
?
Sporothrix carnis
-
Substrates: 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
syringaldazine + O2
?
Sporothrix carnis CPF-05
-
Substrates: 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: the enzyme is able to rapidly decolourize the dye Indigo carmine in the presence of syringaldehyde as a redox mediator, at pH 9.0 and 45°C
Products: -
Products: -
?
syringaldazine + O2
?
Substrates: -
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
syringaldazine + O2
?
-
Substrates: about 17% of the activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Products: -
Products: -
?
tannic acid + O2
?
-
Substrates: 63.1 % of the activity with guaiacol
Products: -
Products: -
?
tannic acid + O2
?
-
Substrates: 63.1 % of the activity with guaiacol
Products: -
Products: -
?
tannic acid + O2
?
-
Substrates: 17.9% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
tyrosine + O2
?
Substrates: the enzyme catalyzes oxidative cross-linking of tyrosine and potato patatin and lysozyme-derived peptides
Products: -
Products: -
?
tyrosine + O2
?
Substrates: the enzyme catalyzes oxidative cross-linking of tyrosine and potato patatin and lysozyme-derived peptides
Products: -
Products: -
?
vanillic acid + O2
4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
Coriolisimus fulvocinerea
-
Substrates: -
Products: -
Products: -
?
vanillic acid + O2
4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
vanillic acid + O2
4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
vanillic acid + O2
4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
vanillic acid + O2
4-carboxy-2-methoxycyclohexa-2,4-dienone + H2O
-
Substrates: -
Products: -
Products: -
?
veratryl alcohol + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
veratryl alcohol + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
? + H2O
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: diphenols not substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: CotA fails to oxidize coumaric acid, cinnamic acid, and vanillic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min
Products: -
Products: -
?
additional information
?
-
Substrates: a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin
Products: -
Products: -
?
additional information
?
-
Substrates: a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin
Products: -
Products: -
?
additional information
?
-
-
Substrates: a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine, catechole, benzidine, 4-cresol, pyrogallol, caffeic acid and tyrosine. Decolorization of structurally different azo dyes using purified laccase: Methyl red, Reactive orange 16, Methyl orange, Sudan IV, Reactive red M5B, Direct brown MR, Reactive red 141, no decolorization of Congo red, Eriochrome black T, and Trypan blue
Products: -
Products: -
?
additional information
?
-
-
Substrates: decolorization of azo dyes: the enzyme decolorizes sulphonyl green BLE up to 97% at pH 7.0 after 2 h incubation at 35°C, without any addition of mediators. No activity with resorcinol and tyrosine
Products: -
Products: -
?
additional information
?
-
-
Substrates: reactive blue 19, reactive black 5 and indigo carmine cand be efficiently decolorized by the purified laccase in the presence of a mediator 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
additional information
?
-
-
Substrates: reactive blue 19, reactive black 5 and indigo carmine cand be efficiently decolorized by the purified laccase in the presence of a mediator 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Products: -
Products: -
?
additional information
?
-
Substrates: two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0
Products: -
Products: -
?
additional information
?
-
-
Substrates: poorly oxidizes m-phenols (resorcinol) and shows no activity towards tyrosine
Products: -
Products: -
?
additional information
?
-
-
Substrates: Malachite green is efficiently degraded by recombinant laccase, especially in combination with mediators
Products: -
Products: -
?
additional information
?
-
-
Substrates: Malachite green is efficiently degraded by recombinant laccase, especially in combination with mediators
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme efficiently performs dye decoloration of anthraquinone dye RBBR
Products: -
Products: -
?
additional information
?
-
-
Substrates: overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with L-tyrosine
Products: -
Products: -
?
additional information
?
-
Substrates: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), as a mediator, is necessary in decolorizing Malachite green
Products: -
Products: -
?
additional information
?
-
-
Substrates: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), as a mediator, is necessary in decolorizing Malachite green
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme shows dye decolorization potential, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme shows dye decolorization potential, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme shows dye decolorization potential, overview
Products: -
Products: -
?
additional information
?
-
Substrates: Lac3 exhibits higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes. Mild synergistic decolorization by the laccases isoenzymes Lac 3 and Lac4 is observed for triphenylmethane dyes but not for anthraquinone and azo dyes
Products: -
Products: -
?
additional information
?
-
Substrates: Lac3 exhibits higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes. Mild synergistic decolorization by the laccases isoenzymes Lac 3 and Lac4 is observed for triphenylmethane dyes but not for anthraquinone and azo dyes
Products: -
Products: -
?
additional information
?
-
-
Substrates: Lac3 exhibits higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes. Mild synergistic decolorization by the laccases isoenzymes Lac 3 and Lac4 is observed for triphenylmethane dyes but not for anthraquinone and azo dyes
Products: -
Products: -
?
additional information
?
-
Substrates: activities are below detection limits for veratryl alcohol, 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) and ferulic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: activities are below detection limits for veratryl alcohol, 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) and ferulic acid
Products: -
Products: -
?
additional information
?
-
Coriolisimus fulvocinerea
-
Substrates: overview substrates, kinetics and stoichiometry
Products: -
Products: -
?
additional information
?
-
-
Substrates: a high-molecular-weight kraft lignin iss effectively polymerized by the enzyme
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases
Products: -
Products: -
?
additional information
?
-
Cubamyces flavidus MTCC-145
-
Substrates: the enzyme transforms toluene and substituted toluenes to corresponding benzaldehyde and substituted benzaldehydes in the absence of mediator molecules with higher catalytic efficiency as compared to other known laccases
Products: -
Products: -
?
additional information
?
-
Substrates: substrate specificity, overview, no activity with tyrosine, phenol, 2,4-dichlorophenol, and 5-sulphosalycylic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview, no activity with tyrosine, phenol, 2,4-dichlorophenol, and 5-sulphosalycylic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: in the presence of metal ions (1 mM Cu2+ and 1 mM Mn2+), the enzyme form preheated at 6-90°C achieves 97% of Malachite green and 98.75% of Indigo blue (both at 2%, w/v) dye decolorization in 12 h. Activity with e.g. Alizarin red S, Congo red, Crystal violet, Eosin, Indigo blue, Lactophenol cotton blue, MG, Methylene blue, and Phenolphthalein, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in decomposition of lignin, discussion of physiological role
Products: -
Products: -
?
additional information
?
-
Substrates: presence of Fe2+ reduces the apparent phenoloxidase activity, Cu(II) enhances this activity six- to sevenfold
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in production of oxygen free radicals
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in decomposition of lignin, discussion of physiological role
Products: -
Products: -
?
additional information
?
-
-
Substrates: LacG decolorizes 43% of Congo red and 14% of Malachite green, and the addition of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as a redox mediator dramatically increases the dye decolorization efficiency
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, no activity with L-tyrosine, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) acts as an effective mediator for amine oxidation by the enzyme. Higher concentrations of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) achieve higher tyramine degradation percentages to nearly 100% with 5 mM
Products: -
Products: -
?
additional information
?
-
Lactiplantibacillus plantarum J16 CECT 8944
-
Substrates: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) acts as an effective mediator for amine oxidation by the enzyme. Higher concentrations of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) achieve higher tyramine degradation percentages to nearly 100% with 5 mM
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in melanin synthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: tyrosine, veratryl alcohol
Products: -
Products: -
?
additional information
?
-
Substrates: Lac1 does not oxidize L-tyrosine
Products: -
Products: -
?
additional information
?
-
Substrates: Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator
Products: -
Products: -
?
additional information
?
-
Lentinus brumalis ibrc05015
Substrates: Lac1 does not oxidize L-tyrosine
Products: -
Products: -
?
additional information
?
-
Lentinus brumalis ibrc05015
Substrates: Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity in descending order: syringaldazine, 4-phenylenediamine, 2,6-dimethoxyphenol, guaiacol, catechol, the purified enzyme decolorizes azo dyes, such as acid red 18, acid Black 1, and direct blue 71, up to 90, 87, and 72%, respectively, optimization, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with L-tyrosine
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with L-tyrosine
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme shows broad substrate specificity. The recombinant His-tagged MmPPOA-695 shows kinetic properties on catechol higher than for known laccases. Laccase belongs to the group of blue, multicopper oxidases which couple the four-electron reduction of dioxygen to water with the oxidation of a broad range of substrates (including phenols, polyphenols, arylamines, anilines, hydroxyindols, and thiols)
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme shows broad substrate specificity. The recombinant His-tagged MmPPOA-695 shows kinetic properties on catechol higher than for known laccases. Laccase belongs to the group of blue, multicopper oxidases which couple the four-electron reduction of dioxygen to water with the oxidation of a broad range of substrates (including phenols, polyphenols, arylamines, anilines, hydroxyindols, and thiols)
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme shows broad substrate specificity. The recombinant His-tagged MmPPOA-695 shows kinetic properties on catechol higher than for known laccases. Laccase belongs to the group of blue, multicopper oxidases which couple the four-electron reduction of dioxygen to water with the oxidation of a broad range of substrates (including phenols, polyphenols, arylamines, anilines, hydroxyindols, and thiols)
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme decolorizes Congo red, Malachite green, Crystal violet, and Methylene green dyes at various decolorization rates of 63-90% without the addition of an expensive mediator
Products: -
Products: -
?
additional information
?
-
FJ560721
Substrates: substrate specificity of purified enzyme, overview. No activity with ferulic acid
Products: -
Products: -
?
additional information
?
-
Opuntia vulgaris
-
Substrates: in the presence of metal ions (1 mM Cu2+ and 1 mM Mn2+), the enzyme form preheated at 6-90°C achieves 97% of Malachite green and 98.75% of Indigo blue (both at 2%, w/v) dye decolorization in 12 h. Activity with e.g. Alizarin red S, Congo red, Crystal violet, Eosin, Indigo blue, Lactophenol cotton blue, MG, Methylene blue, and Phenolphthalein, overview
Products: -
Products: -
?
additional information
?
-
Substrates: Acid Green 27, Acid Violet 7 and Indigo Carmine are used in a dye decolorization assay, overview
Products: -
Products: -
?
additional information
?
-
Substrates: Acid Green 27, Acid Violet 7 and Indigo Carmine are used in a dye decolorization assay, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme possesses a catalytic His residue at the active site, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme possesses a catalytic His residue at the active site, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
Substrates: guaiacol and tannic acid are poorly oxidized. Ferulic and or 3,4-dihydroxyphenylalanine (DOPA) are not oxidized at all. The industrially important dyes Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5 are more effciently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole
Products: -
Products: -
?
additional information
?
-
Substrates: guaiacol and tannic acid are poorly oxidized. Ferulic and or 3,4-dihydroxyphenylalanine (DOPA) are not oxidized at all. The industrially important dyes Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5 are more effciently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme decolorizes fungal melanin and several other dye reagents, overview
Products: -
Products: -
?
additional information
?
-
Substrates: laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties
Products: -
Products: -
?
additional information
?
-
-
Substrates: laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties
Products: -
Products: -
?
additional information
?
-
Phoma sp. UHH 5-1-03 / DSM 22425
Substrates: laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties
Products: -
Products: -
?
additional information
?
-
-
Substrates: the laccase exhibits an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 0.0022 mM
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: no significant enzymic activity with guaiacol
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with tyrosine and hydroquinone, the enzyme in absence of a mediator oxidizes anthracene to anthraquinone to 95%
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with ferulic acid and veratryl alcohol
Products: -
Products: -
?
additional information
?
-
-
Substrates: the isolated enzyme from Pleurotus ostreatus mycelium has both bilirubin oxidase and laccase activities
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with ferulic acid and veratryl alcohol
Products: -
Products: -
?
additional information
?
-
-
Substrates: the isolated enzyme from Pleurotus ostreatus mycelium has both bilirubin oxidase and laccase activities
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with tyrosine and hydroquinone, the enzyme in absence of a mediator oxidizes anthracene to anthraquinone to 95%
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: caffeic acid, ferulic acid, tyrosine
Products: -
Products: -
?
additional information
?
-
-
Substrates: the laccase can effectively decolorize two commercial wool dyes, weak acid blue AS and diamond black PV, up to 88% and 74.7%, respectively, within 2 h in the absence of any redox mediators
Products: -
Products: -
?
additional information
?
-
-
Substrates: the laccase can effectively decolorize two commercial wool dyes, weak acid blue AS and diamond black PV, up to 88% and 74.7%, respectively, within 2 h in the absence of any redox mediators
Products: -
Products: -
?
additional information
?
-
-
Substrates: approximately 66% of the Acid Blue 129 at 100 mg/l is decolorized by 2.5 U of the purified laccase after a 120 min incubation at 50°C, Acid Red 1 at 20 mg/l and Reactive Black 5 at 50 mg/l are decolorized by the purified laccase after the addition of Acid Blue 129 at 100 mg/l.
Products: -
Products: -
?
additional information
?
-
-
Substrates: isozyme laccase II shows a higher activity and wider substrate specificity compared to the other isozymes, overview
Products: -
Products: -
?
additional information
?
-
Substrates: phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity
Products: -
Products: -
?
additional information
?
-
Substrates: phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH. The enzyme catalyses oxidation of nonphenolic compounds more efficiently than that of phenols. The two-domain laccase displays a cytochrome c oxidase activity and exhibits no ferroxidase activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme can efficiently decolourise Indigo Carmine, Coomassie Brilliant Blue R-250, Congo Red, Malachite Green and Alizarin in the presence of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as a redox mediator
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme possesses a catalytic His residue at the active site, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with tyrosine
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzymatic synthesis of morpholine-substituted quinones with cytotoxic, antibacterial and antifungal activities, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity
Products: -
Products: -
?
additional information
?
-
Substrates: the purified enzyme is able to decolourize Green Dye, Orange Dye and Acid Red Dye. The enzyme is unable to decolourize NBB, RBB, or Congo Red. However, with 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) (5 mM) acting as redox mediator, the enzyme decolourizes NBB, RBB, or Congo Red
Products: -
Products: -
?
additional information
?
-
-
Substrates: the purified enzyme is able to decolourize Green Dye, Orange Dye and Acid Red Dye. The enzyme is unable to decolourize NBB, RBB, or Congo Red. However, with 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) (5 mM) acting as redox mediator, the enzyme decolourizes NBB, RBB, or Congo Red
Products: -
Products: -
?
additional information
?
-
Substrates: broad substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: broad substrate specificity
Products: -
Products: -
?
additional information
?
-
Substrates: broad substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: overview substrates, kinetics and stoichiometry
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificities of a laccase chemically modified by attachment of PEG on soluble and cellulose-bound dye substrates, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: polymerization of gliadins by laccase is observed only when a high enzyme dosage and prolonged incubation are used
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: overview substrates, kinetics and stoichiometry
Products: -
Products: -
?
additional information
?
-
-
Substrates: overview substrates, kinetics and stoichiometry
Products: -
Products: -
?
additional information
?
-
-
Substrates: low decolourisation efficiency towards Azure B (1.78 %) and methylene blue (0.38 %)
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview. No activity with tyrosine. Dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process
Products: -
Products: -
?
additional information
?
-
-
Substrates: decolorization of an anthraquinone dye by the enzyme, best at pH 3.0 and 50°C, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview, no activity with tyrosine, phenol, vanillin, and gallic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: degradation of polychlorinated phenols and guaiacols
Products: -
Products: -
?
additional information
?
-
-
Substrates: study on assays and conditions
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is involved in bioremdiation
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in bioremdiation
Products: -
Products: -
?
additional information
?
-
-
Substrates: purified recombinant laccase decolorizes more than 90% of Remazol Brilliant Blue R initially at 80 mg/l1 after 16 h at 45°C and pH 5 when 25 U laccase/ml is used. The purified recombinant laccase can efficiently decolorize Remazol Brilliant Blue R without additional redox mediators
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is involved in bioremdiation
Products: -
Products: -
?
additional information
?
-
-
Substrates: free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator
Products: -
Products: -
?
additional information
?
-
-
Substrates: in the presence of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as the mediator, the enzyme transforms toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitrobenzaldehyde and 4-chlorobenzaldehyde, respectively
Products: -
Products: -
?
additional information
?
-
-
Substrates: in the presence of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as the mediator, the enzyme transforms toluene, 3-nitrotoluene and 4-chlorotoluene to benzaldehyde, 3-nitrobenzaldehyde and 4-chlorobenzaldehyde, respectively
Products: -
Products: -
?
additional information
?
-
-
Substrates: the crude enzyme as well as the purified laccase is able to decolourize dyes from the textile industries, including remazol brilliant blue R, neolane blue and neolane pink
Products: -
Products: -
?
additional information
?
-
E1ACR6
Substrates: the use of rlac1338 and lac2-9 alone had no degradation effect on the industrial dyes [indigo, amaranth, bromophenol blue, acid violet 7, Congo red, coomassie brilliant blue (G250)], however, adding small molecular mediators Ca2+ and 2,2'-azino-bis(3-ethylbenzothiazo-line)-6-sulfonic acid at the same time can significantly improve the degradation ability
Products: -
Products: -
?
additional information
?
-
-
Substrates: poor substrates: guaiacol, catechol, no substrate: tyrosine, L-3,4-dimethoxyphenol, dihydroxyphenylalanine
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DOPA + O2
? + H2O
-
Substrates: the enzyme laccase catalyses the formation of melanin by oxidizing L-DOPA, initiating a series of presumably spontaneous reactions that ultimately leads to the polymerization of the pigment in the yeast cell wall. There, melanin protects the cell from a multitude of environmental and host assaults. Kinetics of dopachrome formation are similar for L- and D-DOPA
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
Substrates: -
Products: -
Products: -
?
4 catechol + O2
4 1,2-benzoquinone + 2 H2O
-
Substrates: -
Products: -
Products: -
?
melanin + O2
?
-
Substrates: the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview
Products: -
Products: -
?
melanin + O2
?
-
Substrates: the enzyme decolorizes fungal melanin from Alternaria alternata strain NBCR 31805 or black hyphae of Cladosporium sp. strain B-1, but not synthetic melanin, overview
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
Oscillatoria subuliformis
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
Oscillatoria subuliformis BDU100712
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
poly R-478 + O2
?
-
Substrates: dye decolorization activity
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in decomposition of lignin, discussion of physiological role
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in production of oxygen free radicals
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in decomposition of lignin, discussion of physiological role
Products: -
Products: -
?
additional information
?
-
-
Substrates: involved in melanin synthesis
Products: -
Products: -
?
additional information
?
-
Substrates: laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties
Products: -
Products: -
?
additional information
?
-
-
Substrates: laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties
Products: -
Products: -
?
additional information
?
-
Phoma sp. UHH 5-1-03 / DSM 22425
Substrates: laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties
Products: -
Products: -
?
additional information
?
-
-
Substrates: the laccase exhibits an inhibitory activity toward HIV-1 reverse transcriptase with an IC50 of 0.0022 mM
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
Substrates: the genes encoding the laccacse isozymes are differently regulated, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes the oxidation of a wide range of compounds, including poly-phenols and anilines. The one electron oxidation of the reducing substrate occurs concomitantly with the four-electron reduction of molecular oxygen to water
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process
Products: -
Products: -
?
additional information
?
-
-
Substrates: laccase couples catalytic oxidation of lignin with the four electron reduction of oxygen, the final product is water without any harmful intermediate making laccase the best candidate for the environmentally benign bleaching process
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is involved in bioremdiation
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in bioremdiation
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is involved in bioremdiation
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Al3+
Ba2+
Ca2+
Cd2+
Co2+
copper
Cr2+
Cu2+
CuSO4
Fe2+
Fe3+
Hg2+
K+
KCl
activity is optimal at 200 mM salt, with 1.5fold greater activity in KCl than in NaCl, and the enzyme displays reduced activity after the removal of salt by dialysis
Li+
Mg2+
Mn2+
Na+
NaCl
Ni2+
Pb2+
Zn2+
additional information
Ba2+
-
about 15% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM
Ca2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Ca2+
-
about 15% loss of maximal activity at 10 mM, about 25% loss of activity at 50 mM
Cd2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Co2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
copper
-
copper content of recombinant CotA laccase from Bacillus subtilis produced by Escherichia coli cells is shown to be strongly dependent on the presence of copper and oxygen in the culture media. In copper-supplemented media, a switch from aerobic to microaerobic conditions leads to the synthesis of a recombinant holoenzyme, while the maintenance of aerobic conditions results in the synthesis of a copper-depleted population of proteins. Strikingly, cells grown under microaerobic conditions accumulate up to 80fold more copper than aerobically grown cells. In vitro copper incorporation into apoenzymes is monitored by optical and electron paramagnetic resonance spectroscopy. This analysis reveals that copper incorporation into CotA laccase is a sequential process, with the type 1 copper center being the first to be reconstituted, followed by the type 2 and the type 3 copper centers
copper
-
4 atoms per protein molecule, a type I CuII, a type II CuII and a type III binuclear CuII
copper
the enzyme is denatured in the presence of a number of denaturing agents and refolded back to functional state with copper. In the folding experiments under alkaline conditions, zinc can replace copper in restoring 100% of laccase activity indicating the non-essential role of copper in this laccase
copper
-
low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper are demonstrated. These observations well explain the copper dependence of CueO oxidase activity
copper
contains six copper atoms per polypeptide chain and displays optical and electron paramagnetic resonance spectra consistent with the presence of type 1, type 2, and type 3 copper centers. The addition of copper leads to immediate and reversible changes in the optical and electron paramagnetic resonance spectra of the protein, as well as decreased thermal stability of the enzyme and stimulates both the phenoloxidase and ferroxidase activities
copper
four copper atoms per molecule, spectroscopic properties are typical of blue copper oxidase. Presence of copper is essential for CueO-dependent oxidation of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) and p-phenylenediamine
copper
mechanism of copper incorporation in CueO is sequential, with type 1 copper being the first to be reconstituted, followed by type 2 and type 3 sites. The copper content of the purified protein is routinely 0.5-0.6 Cu atoms/protein molecule
copper
-
10 mM increase activity by 17%, 0.1 mM increase activity by 100%
copper
multicopper oxidase. The apoenzyme is inactive. Incubation with CuSO4 allows a 43fold increase of the specific activity yielding a metallo-enzyme
copper
the enzyme is modified posttranslationally, including removal of 31 amino acid residues from its N terminus, addition of glycan residues (glycosylation), and apparent coordination of four copper atoms into three types of copper-binding sites
copper
-
16 gatom per mol, laccase I, 4 gatom per mol, laccases II, III, study of copper centers
copper
-
X-ray absorption edge study of type I, type II and binuclear type III copper center
copper
-
X-ray absorption spectra, possible copper-copper interaction
Cu2+
activity is low in the absence of Cu2+, so 1 mM Cu2+ is added into the assay buffer
Cu2+
-
the CotA-type laccase is associated with 4 Cu2+ ions, content determined by inductive coupled plasma optical emission spectroscopy
Cu2+
-
a copper enzyme, the wild-type enzyme contains 3.7 mol Cu2+ per mol of enzyme, mutants I494A and L386A contain 4.0 mol copper per mol of enzyme. Binding structures in wild-type and mutant enzymes I494A and L386A, analysis of crystal structures and simulation, overview
Cu2+
-
required, stimulates up to 0.2 mM, contains type I Cu+ and type III Cu+
Cu2+
zinc can replace copper in restoring 100% of laccase activity indicating the non-essential role of copper in this laccase
Cu2+
multicopper enzyme, copper is not fully incorporated into the type-I Cu center of Escherichia coli purified enzyme. Typical metal content of laccases includes a type-1 Cu site (T1), a type-2 Cu site (T2), and a dinuclear type-3 Cu site (T3), with T2 and T3 arranged in a trinuclear cluster. The T1 Cu site contains the blue copper, whose tight coordination to a cysteine is responsible for an intense SCys -> Cu(II) charge transfer transition at around 600 nm, giving the typical blue color to the enzyme. T2 shows a characteristic electron paramagnetic resonance (EPR) spectrum, clearly distinct from that of T1, whereas the T3 copper dimer is anti-ferromagnetically coupled and EPR-silent
Cu2+
-
presence of type-1 site and type-3 binuclear copper in the bright blue colored purified enzyme
Cu2+
four coppers involved in dioxygen binding, a copper T1 forming a mononuclear site and a cluster of three coppers T2, T3, and T3' forming a trinuclear site, binding and coordination structure analysis, overview
Cu2+
-
the enzyme contains 1 mol of Fe and Zn and 2 mol of Cu per mol of protein
Cu2+
His64 and His399 coordinate with T2 Cu and the other six histidines at positions 66, 109, 111, 401, 451 and 453 coordinate with the T3 Cu pair, a hydrogen bond between Glu460 and Ser113 causes a longer Cu1-N-His458 bond at T1 site, Cu2+ binding structure, overview
Cu2+
-
activates, increases the enzyme activity best at 0.25 mM in the culture medium
Cu2+
laccases contain four copper ions distributed into three sites that, according to their spectroscopic properties, are classified as type 1 Cu, or blue copper centre, type 2 or normal copper centres, and type 3 or coupled binuclear copper centres, overview
Cu2+
-
stimulates the wild-type enzyme by 6% at 10 mM, 14.5% at 100 mM, required for activity
Cu2+
-
laccase activity is increased 2.6-fold by the addition of 10 mM copper sulfate
Cu2+
copper-containing enzyme. Contains 4 atoms per molecule of the enzyme
Cu2+
the enzyme requires copper ions for activity. The dependence of the activity on Cu2+ concentration is sigmoidal with the midpoint at 0.00304 mM. Other metal ions (Mg2+, Mn2+, Ca2+, Ni2+, or Zn2+ each at 1 mM) fail to support the activity. The methionine-rich region from Met271 to Met283 may be involved in copper binding
Cu2+
-
copper-containing enzyme, trinuclear Cu cluster upon interaction with o-fluorophenol, overview
Cu2+
-
type 1 copper ion, Redox potential: 790 mV, 4 ions per enzyme molecule
Cu2+
-
the structure of laccase active sites including all copper centers of types T1, T2, and T3 changes during the phase transition, overview
Cu2+
-
a multicopper enzyme, redox potential determination of the different copper ions of the different isozymes, overview
Cu2+
the enzyme contains 4 copper molecules per enzyme molecule, structure overview
Fe2+
-
the enzyme contains 1 mol of Fe and Zn and 2 mol of Cu per mol of protein
K+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Mg2+
-
about 10% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM
Mn2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Mn2+
-
about 10% loss of maximal activity at 10 mM, about 20% loss of activity at 50 mM
Na+
-
up to 400 mM NaCl stimulates laccase activity. At 600 to 800 mM NaCl, the laccase retains all of its original activity and at higher concentrations, the activity decreases
Na+
-
exhibits activation of laccase up to 1 mM beyond which it exhibited inhibition of laccase activity
NaCl
activity is optimal at 200 mM salt, with 1.5fold greater activity in KCl than in NaCl, and the enzyme displays reduced activity after the removal of salt by dialysis. Also active at relatively high concentrations of salt, with 65% activity at 1 M NaCl
NaCl
native wild-type MmPPOA and recombinant wild-type MmPPOA-695-His (both the crude extract and the pure enzyme preparations) retain substantial part of the enzymatic activity in the presence of 1 M NaCl, while recombinant mutant His-tagged MmPPOA-637 activity shows a 90% decrease at 100 mM NaCl and about 20% increase at 1 M NaCl
Zn2+
zinc can replace copper in restoring 100% of laccase activity indicating the non-essential role of copper in this laccase
Zn2+
-
the enzyme contains 1 mol of Fe and Zn and 2 mol of Cu per mol of protein
additional information
-
no activation or inhibition by 1 mM of MgCl2, CaCl2, MnCl2, ZnSO4, or CuSO4
additional information
except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity
additional information
-
except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity
additional information
metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzymes Lac3 and Lac4
additional information
metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzymes Lac3 and Lac4
additional information
-
metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzymes Lac3 and Lac4
additional information
metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzyme Lac4
additional information
metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzyme Lac4
additional information
-
metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzyme Lac4
additional information
-
the enzyme activity is not affected by Ca2+ at 2 mM, and by NaCl up to 1.2 M
additional information
FJ560721
laccase activity remains stable for 24 h against all the ions tested, i.e. Cu2+, Co2+, Pb2+, Ca2+, Cd2+, Mg2+, Mn2+, Fe2+, Ni2+, F-, Zn2+, and Li+, at 10-100 mM
additional information
-
the enzyme activity is not affected by 7 mM Mn2+ and 1 mM H2O2, and by 1 mM of Ag+, Ba2+, Ca2+, Mg2+, Zn2+, Co2+, Ni2+ or Cu2+ or by sulfhydryl or chelating reagents
additional information
-
no or poor effect on enzyme activity by K+, Ca2+, Cu2+, Mg2+, Mn2+, Zn2+, and Ba2+ at 10 mM
additional information
-
the laccase is highly metal-tolerant. No or poor effects by Mn2+, Na+, Zn2+, Mg2+, Ca2+, K+, Fe2+, and Fe3+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
shows substrate inhibition at high concentrations above 6 mM
2,2-dipyridyl
1 mM, 13.4% loss of activity. 10 mM, 24.8% loss of activity
4-hydroxyphenylacetic acid
-
effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-hydroxyphenylpropionic acid
-
effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-nitrophenol
-
effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
Benzene
-
1 h, 20% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v)
chitosan
-
conjugated to the enzyme, with 81-93% laccase being conjugated, a moderate activity loss of 16-28% occurs in conjugation solution, a second severe activity loss of 63-78% occurs during a cycle of phase change consisting of precipitation, centrifugation and re-dissolution of the enzymechitosan conjugates, the chitosan molecular size has little effect, overview
DL-dithiothreitol
0.01 mM, 9.5% loss of activity. 0.1 mM, complete loss of activity
N,N-Dimethylformamide
-
1 h, 10% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v)
1,10-phenanthroline
1 mM, 14.7% loss of activity. 10 mM, 89.1% loss of activity
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
-
99.5% inhibition
2-mercaptoethanol
-
complete inhibition of laccase activity at 0.1% concentration
Ca2+
-
1% residual activity at 10 mM; 86.6% residual activity at 10 mM
Ca2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Cd2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
chloroform
-
1 h, 20% loss of activity by 20% (v/v), 65% loss of activity by 50% (v/v)
Co2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
diethyldicarbonate
-
inactivation in simple linear pseudo-first order kinetics, recovery by 0.5 M hydroxylamine
diethyldicarbonate
-
inactivation in simple linear pseudo-first order kinetics, recovery by 0.5 M hydroxylamine
diethyldicarbonate
-
inactivation in simple linear pseudo-first order kinetics, recovery by 0.5 M hydroxylamine
dimethyl sulfoxide
-
1 h, 10% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v)
dithiothreitol
-
5 mM, complete inactivation. 1 mM, 80% loss of activity
dithiothreitol
-
activates laccase at lower concentration (0.1 mM) while inhibits the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition of activity at 10 mM
dithiothreitol
0.1 mM, complete inactivation; 0.1 mM, complete inactivation
DTT
-
complete inhibition at over 10 mM with substrate 3,6-dimethoxyphenol and at over 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
EDTA
50 mM, 70% loss of activity, laccase 3; 50 mM, 80% loss of activity, laccase 4
EDTA
the reaction in the presence of 0.1 mM CuSO4 is completely inhibited by the addition of 1 mM EDTA
ethanol
-
1 h, 20% loss of activity by 20% (v/v), 45% loss of activity by 50% (v/v)
Isopropanol
-
1 h, 40% loss of activity by 20% (v/v), 65% loss of activity by 50% (v/v)
K+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
L-cysteine
0.1 mM, 17% loss of activity. 1 mM, complete loss of activity
L-cysteine
-
89% inhibition at 0.1 mM, complete inhibition at 1 mM
methanol
-
1 h, 20% loss of activity by 20% (v/v), 60% loss of activity by 50% (v/v)
Mn2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Na+
-
exhibits activation of laccase up to 1 mM beyond which it exhibits inhibition of laccase activity
NaCl
10 mM, 20% inhibition. 500 mM, 63% inhibition. 1000 mM, complete loss of activity
NaCl
-
50% inhibition at 20 mM, the inhibitory effect of chloride ions is reversible
NaF
-
complete inhibition at over 10 mM with substrates 3,6-dimethoxyphenol and 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
NaN3
10 mM, residual activity of wild-type enzyme is 23%, residual activity of mutant enzyme M199A is 13%, residual activity of mutant enzyme M199G is 20%, residual activity of mutant enzyme Y230A is 20%, residual activity of mutant enzyme H165A/M199G is 1116%, residual activity of mutant enzyme H165A/R240H is 78%, residual activity of mutant enzyme M199G/R240H is 25%
NaN3
-
complete inhibition at over 80 mM with substrate 3,6-dimethoxyphenol and at over 40 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
Sodium azide
-
complete inhibition of laccase activity at 0.1% concentration
thioglycolic acid
-
93% inhibition at 0.1 mM, complete inhibition at 1 mM
Thiourea
1 mM, 4.5% loss of activity. 10 mM, 90.4% loss of activity
additional information
Mn2+ even at 20 mM does not affect Atm activity
-
additional information
no inhibition: EDTA (10-50 mM), SDS (0.1-1 mM)
-
additional information
-
the enzyme can tolerate up to 600-800 mM NaCl. NaN3 (1 mM) has almost no effect on the laccase activity
-
additional information
no significant loss of activity loss at 10 mM EDTA
-
additional information
except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity
-
additional information
-
except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity
-
additional information
isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS
-
additional information
isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS
-
additional information
-
isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS
-
additional information
-
5 mM potassium cyanide shows no inhibitory effect on the laccase
-
additional information
-
no inhibition by 0.1 mM EDTA, laccase retains 100 % activity with 2,6-dimethoxyphenol as a substrate in the presence of clorgiline or pargyline (specific amine oxidase inhibitors)
-
additional information
the recombinant His-tagged MmPPOA-695 shows a strong resistance to NaCl, DMSO, and Tween-80
-
additional information
-
the recombinant His-tagged MmPPOA-695 shows a strong resistance to NaCl, DMSO, and Tween-80
-
additional information
-
no inhibition by iodoacetate and chloroacetamide
-
additional information
-
no inhibition by iodoacetate and chloroacetamide
-
additional information
the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF
-
additional information
-
the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF
-
additional information
-
no inhibition by iodoacetate and chloroacetamide
-
additional information
-
strongly inhibited by Cu-chelating agents
-
additional information
-
no or poor inhibition by Co2+, Zn2+, Sn2+, Mn2+, Mg2+, K+, Fe2+, Ba2+, and Na+
-
additional information
-
soluble laccase shows more than 80% relative activity in organic solvents like ethanol, methanol, toluene and acetonitrile
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-chlorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
2-Fluorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-Chlorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-Fluorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
acetate
the oxidizing activity of CueO for ABTS is enhanced by the addition of up to 300 mM acetate ion, although it decreases at higher concentrations
acetic acid
FpLcc2 is activated by acetic acid up to 3.7times. This activation effect is much more pronounced at pH 5.0 compared to pH 3.0 and can already be observed at a concentration of 1 mM
CuSO4
-
activates 113% at over 40 mM with substrate 3,6-dimethoxyphenol, and 46% at over 10 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
dithiothreitol
-
activates laccase at lower concentration (0.1 mM) while inhibites the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition inactivity at 10 mM
MnSO4
-
activates 49% at over 80 mM with substrate 3,6-dimethoxyphenol, and 28% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
oxalic acid
-
activates 42% at 2 mM with substrate 3,6-dimethoxyphenol, and 303% at 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
10-(3-propylsulfonate) phenoxazine
-
i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation
10-(3-propylsulfonate) phenoxazine
-
i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation
10-(3-propylsulfonate) phenoxazine
-
i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation
10-(3-propylsulfonate) phenoxazine
-
i.e. PSPX, activates 541times, model of synergistic substrate TEMPO and PSPX oxidation
Cu2+
-
activates, increases the enzyme activity best at 0.05 mM in the culture medium
EDTA
-
activates 10% at over 80 mM with substrate 3,6-dimethoxyphenol, and 50% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
additional information
-
highest induction by 2,6-dimethylaniline, but also by 3,5-dihydroxybenzoic acid, guaiacol, 4-hydroxy-3-methoxycinnamic acid, calcium lignosulphonate, 2,5-dimethylaniline, 3,4-dimethylaniline, 3,4-dimethoxybenzaldehyde, 4-hydroxybenzaldehyde, and 2,6-dihydroxybenzoic acid, overview
-
additional information
-
the extracellular laccase production is enhanced up to 2.5-fold in the medium amended with xylidine at 1 mM
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.19
2,2'-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid)
-
pH 6.0, 50°C
0.0095
2,2,6,6-tetramethyl-piperidine-1-oxyl radical
-
pH 5.0, 25°C, recombinant enzyme
0.67
2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
45°C, pH 6.0, LccA protein isolated from Haloferax volcanii H26 strain US02
0.66
3,4-dihydroxy L-phenylalanine
in McIlvaine buffer (pH 4.0), at 30°C
0.236
bilirubin
45°C, pH 8.4, LccA protein isolated from Haloferax volcanii H26 strain US02
34.1
1-hydroxybenzotriazole
pH 5.0, temperature not specified in the publication
47.7
1-hydroxybenzotriazole
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
5.6
1-naphthol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
6.04
1-naphthol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.07
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 3.0, 22°C, recombinant wild-type enzyme
0.086
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, recombinant wild-type enzyme
0.102
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 22°C, deglycosylated enzyme
0.106
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 22°C, native enzyme
0.11
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 3.0, 22°C, recombinant truncated mutant POXA1bDELTA4
0.11
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 3.0, 22°C, recombinant truncated mutant POXA1bDELTA16
0.112
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, recombinant mutant WlacS
0.124
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, wild-type CotA
0.129
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, recombinant mutant WlacD
0.145
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, mutant L386A
0.18
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.5, 35°C
0.26
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 22°C, recombinant enzyme expressed in Saccharomyces cerevisiae
0.28
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 25°C, recombinant enzyme expressed in Trichoderma reesei
0.32
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 3.0, 22°C, recombinant truncated mutant POXA1bD205R
0.4
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 25°C, recombinant wild-type enzyme
0.9
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae
2.027
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, mutant I494A
0.0107
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate)
-
pH 3.0, 25°C, laccase Lac-3.5
0.017
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate)
-
pH 3.0, 25°C,laccase Lac-4.8
0.0013
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, temperature not specified in the publication
0.002
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, temperature not specified in the publication, wild-type PM1 laccase
0.0045
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3, temperature not specified in the publication, mutant enzyme V159E
0.0046
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3, temperature not specified in the publication, mutant enzyme V159E/N398D
0.00866
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 25°C
0.02
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, temperature not specified in the publication
0.024
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH not specified in the publication
0.0509
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 30°C
0.0595
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C
0.075
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme H165A/R240H, 30°C, pH not specified in the publication
0.075
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme H165A/R240H
0.076
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.09
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme H165A/M199G, 30°C, pH not specified in the publication
0.09
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme H165A/M199G
0.1
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199G/R240H, 30°C, pH not specified in the publication
0.1
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme M199G/R240H
0.1
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH not specified in the publication
0.17
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme Y230A, 30°C, pH not specified in the publication
0.17
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme Y230A
0.2
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199G, 30°C, pH not specified in the publication
0.2
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, mutant enzyme M199G
0.21
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
0.32
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199A, 30°C, pH not specified in the publication
0.32
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, mutant enzyme M199A
0.36
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
wild-type enzyme, 30°C, pH not specified in the publication
0.36
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, wild-type enzyme
0.394
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 5.0, 30°C, soluble enzyme
0.62
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
25°C, pH 5.6
0.747
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 5.0, 30°C, surface display laccase (SDL) biocatalyst (where the enzyme laccase is displayed on the surface of biological cells through synthetic biology)
0.9
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 30°C
2.12
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Sphingobacterium sp. ksn-11
-
pH 4.5, 40°C
3.99
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
70°C, pH 3.0
11.99
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 2.5, 55°C
20.1
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C
22.4
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 30°C
0.0023
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
in 67 mM sodium acetate buffer (pH 4.5), at 30°C
0.0033
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 50°C
0.014
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
0.02
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
in McIlvaine buffer (pH 4.0), at 30°C
0.028
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
0.034
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
0.035
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
0.036
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
FJ560721
pH 5.0, 22-25°C
0.041
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C
0.045
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C
0.0552
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 6.0, 25°C
0.058
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant K532A
0.07
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
isoform POXA3a, pH 3.6, 25°C
0.074
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
isoform POXA3b, pH 3.6, 25°C
0.0794
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
at 30°C in 100 mM sodium tartrate buffer (pH 4.5)
0.08
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 22°C, recombinant enzyme
0.084
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant K532E
0.086
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 30°C
0.09
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
isoform POXA1w, pH 3.0, 25°C
0.104
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant enzyme, pH 3.0, 30°C
0.105
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant P530A
0.12
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
isoform POXA2, pH 3.0, 25°C
0.13
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
0.15
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, temperature not specified in the publication
0.19
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.0, 50°C
0.203
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
in 100 mM citrate buffer, pH 4.5, at 37°C
0.28
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
isoform POXC, pH 3.0, 25°C
0.28
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
0.307
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.2, 37°C
0.37
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
isoform POXA1b, pH 3.0, 25°C
0.535
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
at 22°C, pH not specified in the publication
0.653
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant K532A
0.7
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
40°C, pH 4.5
0.8158
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 2.5, 25°C
1.219
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant P530A
1.278
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant K532E
2.174
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant K532A
2.487
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant K532E
3.703
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant P530A
4.317
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
7.611
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
0.00732
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 25°C
0.01
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH and temperature not specified in the publication
0.027
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.4, 70°C
0.0316
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
Sporothrix carnis
-
pH 7.5, 30°C
0.042
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH and temperature not specified in the publication
0.046
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C, recombinant enzyme
0.0934
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH and temperature not specified in the publication
0.11
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, wild-type enzyme
0.1107
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188R, in aqueous solution
0.1151
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188I, in aqueous solution
0.129
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188L, in aqueous solution
0.1327
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 22°C
0.1329
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 30°C
0.1329
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188V, in aqueous solution
0.136
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, isoenzyme Lac3
0.1419
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, wild-type enzyme, in aqueous solution
0.149
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 37°C
0.1511
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188K, in aqueous solution
0.1714
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188A, in aqueous solution
0.205
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, isoenzyme Lac4
0.21
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 7.4, 37°C
0.2308
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 4.5
0.25
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4, 30°C
0.28
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.0, 30°C
0.358
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
45°C, pH 5.0
0.366
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
0.43
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 55°C
0.5
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 6, 25°C, soluble enzyme
1.17
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
1.26
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
laccase DcLac2, pH 2.8, temperature not specified in the publication
1.4
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 8.0, 85°C
2
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 6, 25°C,laccase immobilized in solgel matrix
2.06
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I/V482G 2.06 (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
3.9
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
laccase DcLac1, pH 2.8, temperature not specified in the publication
4.5
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, temperature not specified in the publication
5.33
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 35°C
6.86
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 7.0 37°C
0.9
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
pH and temperature not specified in the publication
2.4
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
60°C, pH 6.0
3.8
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
55°C, pH 6.0
4.4
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
50°C, pH 6.0
0.00134
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 50°C, free enzyme
0.005
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 50°C, enzyme conjugated with 1.1 kDa PEG
0.00632
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 50°C, enzyme conjugated with 2 kDa PEG
0.00704
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 50°C, enzyme conjugated with 5 kDa PEG
0.01
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant mutant D206A
0.0113
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5
0.0128
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3.0, 50°C
0.016
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant mutant D206N
0.026
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant mutant D206E
0.03
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
isoenzyme lac90
0.03
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3.0, 45°C
0.038
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 5.0, 22°C
0.038
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 25°C
0.041
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 4.5, 65°C
0.0423
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
free enzyme
0.045
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 5.5, 20°C
0.0452
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 505 kDa
0.049
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.6, 37°C, mutant M502F
0.0498
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 214 kDa
0.05
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant wild-type enzyme
0.058
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.2, 50°C
0.07
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
25°C, isozyme POXA3a
0.074
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
25°C, isozyme POXA3b
0.077
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3.0, 25°C
0.0852
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 432 kDa
0.087
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.6, 37°C, wild-type enzyme
0.089
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.6, 37°C, mutant M502L
0.095
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 131 kDa
0.11
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.0, 25°C
0.21
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 40°C
0.41
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Phlebia fascicularia
-
Lac II, 70°C, pH 2.2
0.42
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Phlebia fascicularia
-
Lac I, 70°C, pH 2.2
48.6
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.2, 45°C
0.022
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
G2H480
pH 7.0, 25°C
0.31
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant M455L, 30°C, pH 5
1.3
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant D439A/M510L, pH 5.5, temperature not specified in the publication
1.4
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant P444A/M510Q, pH 5.5, temperature not specified in the publication
1.58
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, presence of 0.5 mM Cu2+, 30°C, pH 5
1.88
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant G304K, pH 5.5, 25°C
2.33
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant M456A, presence of 0.5 mM Cu2+, 30°C, pH 5
2.94
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, 30°C, pH 5
3.79
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, pH 5.5, 25°C
6.4
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
6.5
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, pH 5.5, 25°C
11
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
12
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion mutant, pH 5.5, 25°C
1.163
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C
1.263
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
recombinant wild-type enzyme MmPPOA-695, pH 5.0, 25°C
1.84
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
native wild-type enzyme, pH 5.0, 25°C
0.011
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
22°C, pH 5.0
0.0392
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 6.0, 40°C
0.0536
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
25°C, pH 4.5
0.722
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, C-6His tagged laccase 3
0.835
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, deglycosylated enzyme
0.964
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA))
1.237
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H)
1.268
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)), 6His tagged (C(DELTA)6H) and hyper-glycosylated
2.042
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, wild-type enzyme
12
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 23°C
45
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 23°C
4.2
2,5-diaminotoluene
mutant M510L, pH 5.5, temperature not specified in the publication
5.5
2,5-diaminotoluene
mutant D439A/M510L, pH 5.5, temperature not specified in the publication
6.7
2,5-diaminotoluene
mutant D439A/P444A, pH 5.5, temperature not specified in the publication
9.3
2,5-diaminotoluene
mutant P444A, pH 5.5, temperature not specified in the publication
12
2,5-diaminotoluene
wild-type, pH 5.5, temperature not specified in the publication
12
2,5-diaminotoluene
mutant D439A, pH 5.5, temperature not specified in the publication
14
2,5-diaminotoluene
mutant P444A/M510Q, pH 5.5, temperature not specified in the publication
0.0086
2,6-dimethoxyphenol
-
at 30°C in 100 mM sodium tartrate buffer (pH 4.5)
0.01
2,6-dimethoxyphenol
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
0.011
2,6-dimethoxyphenol
pH 4.5, 25°C, recombinant wild-type enzyme
0.016
2,6-dimethoxyphenol
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae
0.016
2,6-dimethoxyphenol
-
pH 5, temperature not specified in the publication, mutant enzyme V159E
0.02
2,6-dimethoxyphenol
30°C, pH not specified in the publication
0.027
2,6-dimethoxyphenol
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
0.032
2,6-dimethoxyphenol
wild-type enzyme, 30°C, pH not specified in the publication
0.05
2,6-dimethoxyphenol
pH 5.0, temperature not specified in the publication
0.053
2,6-dimethoxyphenol
-
at 22°C, pH not specified in the publication
0.08
2,6-dimethoxyphenol
-
pH 5, temperature not specified in the publication, mutant enzyme V159E/N398D
0.1
2,6-dimethoxyphenol
30°C, pH not specified in the publication
0.15
2,6-dimethoxyphenol
mutant enzyme H165A/R240H, 30°C, pH not specified in the publication
0.15
2,6-dimethoxyphenol
30°C, pH 9.0, mutant enzyme H165A/R240H
0.16
2,6-dimethoxyphenol
-
pH and temperature not specified in the publication
0.172
2,6-dimethoxyphenol
recombinant wild-type enzyme MmPPOA-695, pH 5.0, 25°C
0.19
2,6-dimethoxyphenol
pH 3.4, 30°C, recombinant wild-type enzyme
0.2
2,6-dimethoxyphenol
pH and temperature not specified in the publication
0.25
2,6-dimethoxyphenol
mutant enzyme M199G/R240H, 30°C, pH not specified in the publication
0.25
2,6-dimethoxyphenol
30°C, pH 8.5, mutant enzyme M199G/R240H
0.28
2,6-dimethoxyphenol
mutant enzyme H165A/M199G, 30°C, pH not specified in the publication
0.28
2,6-dimethoxyphenol
30°C, pH 6.5, mutant enzyme H165A/M199G
0.293
2,6-dimethoxyphenol
recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C
0.324
2,6-dimethoxyphenol
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
0.38
2,6-dimethoxyphenol
pH 8.5, temperature not specified in the publication
0.41
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, wild-type enzyme
0.43
2,6-dimethoxyphenol
pH 5.3, 22°C, recombinant truncated mutant POXA1bDELTA4
0.43
2,6-dimethoxyphenol
pH 5.3, 22°C, recombinant truncated mutant POXA1bDELTA16
0.5
2,6-dimethoxyphenol
pH and temperature not specified in the publication
0.503
2,6-dimethoxyphenol
-
pH 5.0, temperature not specified in the publication
0.56
2,6-dimethoxyphenol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.57
2,6-dimethoxyphenol
pH 5.3, 22°C, recombinant wild-type enzyme
0.602
2,6-dimethoxyphenol
-
pH and temperature not specified in the publication
0.654
2,6-dimethoxyphenol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
0.67
2,6-dimethoxyphenol
mutant enzyme M199G, 30°C, pH not specified in the publication
0.67
2,6-dimethoxyphenol
30°C, pH 8.0, mutant enzyme M199G
0.76
2,6-dimethoxyphenol
mutant enzyme Y230A, 30°C, pH not specified in the publication
0.76
2,6-dimethoxyphenol
30°C, pH 8.5, mutant enzyme Y230A
0.83
2,6-dimethoxyphenol
mutant enzyme M199A, 30°C, pH not specified in the publication
0.83
2,6-dimethoxyphenol
30°C, pH 8.0, mutant enzyme M199A
1.176
2,6-dimethoxyphenol
-
in 100 mM citrate buffer, pH 4.5, at 37°C
1.26
2,6-dimethoxyphenol
pH 5.3, 22°C, recombinant truncated mutant POXA1bD205R
1.413
2,6-dimethoxyphenol
recombinant mutant enzyme MmPPOA-637, pH 5.0, 25°C
1.8
2,6-dimethoxyphenol
region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
1.83
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
1.98
2,6-dimethoxyphenol
native wild-type enzyme, pH 5.0, 25°C
2.16
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
2.5
2,6-dimethoxyphenol
wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
3.295
2,6-dimethoxyphenol
recombinant His-tagged mutant enzyme MmPPOA-637, pH 5.0, 25°C
0.0004
3-(4-dimethylamino-1-phenylazo) benzene sulfonic acid sodium salt
-
soluble enzyme in presence of 1-hydroxybenzotriazole
0.0007
3-(4-dimethylamino-1-phenylazo) benzene sulfonic acid sodium salt
-
immobilized enzyme in presence of 1-hydroxybenzotriazole
0.0082
3-(4-dimethylamino-1-phenylazo) benzene sulfonic acid sodium salt
-
soluble enzyme
0.0315
3-(4-dimethylamino-1-phenylazo) benzene sulfonic acid sodium salt
-
immobilized enzyme
0.069
4-hydroxy-3,5-dimethoxybenzaldehyde azine
pH 5.0, 25°C
0.092
4-hydroxy-3,5-dimethoxybenzaldehyde azine
-
pH and temperature not specified in the publication
1.41
4-hydroxybenzoic acid
pH 5.0, temperature not specified in the publication
1.96
4-hydroxybenzoic acid
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
0.062
acetaminophen
-
pH 5.0, 30°C, surface display laccase (SDL) biocatalyst (where the enzyme laccase is displayed on the surface of biological cells through synthetic biology)
0.018
catechol
recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C
0.022
catechol
recombinant wild-type enzyme MmPPOA-695, pH 5.0, 25°C
1.478
catechol
recombinant mutant enzyme MmPPOA-637, pH 5.0, 25°C
0.24
guaiacol
22°C, pH 7.0, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
0.24
guaiacol
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
0.3
guaiacol
pH 8.5, temperature not specified in the publication
1.39
guaiacol
22°C, pH 7.0, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
2.23
guaiacol
pH and temperature not specified in the publication
4.3
guaiacol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
4.9
guaiacol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
0.17
K4[Fe(CN)6]
30°C, pH 4.0, mutant enzyme M199G/R240H
0.18
K4[Fe(CN)6]
30°C, pH 4.0, mutant enzyme H165A/R240H
0.24
K4[Fe(CN)6]
30°C, pH 4.0, mutant enzyme H165A/M199G
9.9
K4[Fe(CN)6]
pH 4.0, temperature not specified in the publication
0.84
L-Dopa
-
enzyme has a higher affinity for syringaldazine than for L-dopa
0.53
N,N-dimethyl-1,4-phenylenediamine
-
pH 4, temperature not specified in the publication, mutant enzyme V159E
1.7
N,N-dimethyl-1,4-phenylenediamine
-
pH 4, temperature not specified in the publication, mutant enzyme V159E/N398D
0.29
N,N-Dimethyl-p-phenylenediamine
pH 4.0, temperature not specified in the publication
1.06
N,N-Dimethyl-p-phenylenediamine
pH 4.0, temperature not specified in the publication, wild-type PM1 laccase
0.43
N-acetyldopamine
pH 5.0-5.5, recombinant N-terminally truncated enzyme
0.144
o-Dianisidine
30°C, pH not specified in the publication
0.647
o-Dianisidine
30°C, pH not specified in the publication
90
p-phenylenediamine
region Pro357-His406 deletion mutant, pH 5.5, 25°C
0.013
Sinapic acid
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
0.085
Sinapic acid
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
0.005
syringaldazine
-
at 22°C, pH not specified in the publication
0.0081
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H)
0.0126
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H) and hyper-glycosylated
0.0196
syringaldazine
pH 6.5, 37°C, recombinant enzyme
0.0237
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA))
0.03
syringaldazine
pH 6.0, 22°C, recombinant truncated mutant POXA1bDELTA4
0.03
syringaldazine
pH 6.0, 22°C, recombinant truncated mutant POXA1bDELTA16
0.031
syringaldazine
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae
0.035
syringaldazine
45°C, pH 6.0, LccA protein isolated from Haloferax volcanii H26 strain US02
0.037
syringaldazine
pH 4.5, 25°C, recombinant wild-type enzyme
0.04
syringaldazine
30°C, pH not specified in the publication
0.101
syringaldazine
30°C, pH not specified in the publication
0.11
syringaldazine
-
enzyme has a higher affinity for syringaldazine than for L-dopa
1.88
syringaldazine
pH and temperature not specified in the publication
3.77
syringaldazine
pH and temperature not specified in the publication
additional information
additional information
-
comparison of values for free and immobilized laccase
-
additional information
additional information
-
study on kinetics, stoichiometries
-
additional information
additional information
-
study on kinetics, stoichiometries
-
additional information
additional information
-
study on kinetics, stoichiometries
-
additional information
additional information
Coriolisimus fulvocinerea
-
study on kinetics, stoichiometries
-
additional information
additional information
-
kinetic studies of electron transfer
-
additional information
additional information
-
dependency on source of enzyme, pH, inhibition by F-
-
additional information
additional information
-
dependency on source of enzyme, pH, inhibition by F-
-
additional information
additional information
-
dependency on source of enzyme, pH, inhibition by F-
-
additional information
additional information
-
dependency on source of enzyme, pH, inhibition by F-
-
additional information
additional information
-
dependency on source of enzyme, pH, inhibition by F-
-
additional information
additional information
-
kinetics of isozymes, overview
-
additional information
additional information
-
thermodynamics of wild-type and mutant enzymes, overview
-
additional information
additional information
Fomes sclerodermeus
-
both isozymes show Michaelis-Menten kinetics, overview
-
additional information
additional information
-
MichaelisMenten kinetics, laccases copper type I redox potential
-
additional information
additional information
-
MichaelisMenten kinetics, laccases copper type I redox potential
-
additional information
additional information
-
MichaelisMenten kinetics, laccases copper type I redox potential
-
additional information
additional information
-
Michaelis-Menten kinetics, laccases copper type I redox potential
-
additional information
additional information
-
kinetics with Michaelis-Menten constants obtained from amperometric measurements, kinetics of coupled reaction with catechol and 1-hydroxybenzotriazole, overview
-
additional information
additional information
-
kinetics in absence and presence of acidic and halophenols, overview
-
additional information
additional information
-
transient/stopped-flow and steady-state kinetics, modeling
-
additional information
additional information
-
analysis of enzyme dynamics and catalytic, spectral properties. Analysis of the structural state of the purified native laccase in solution at the phase transition before freezing, at freezing, and subsequent thawing of enzyme solution, processes in the active site on environmental temperature changes, overview
-
additional information
additional information
the enzyme shows typical MichaelisMenten kinetics, overview
-
additional information
additional information
-
the enzyme shows typical MichaelisMenten kinetics, overview
-
additional information
additional information
construction of enzymesubstrate complexes and molecular dynamics simulations, complex of mutant D205R with 2,5-xylidine, molecular dynamics simulations, overview
-
additional information
additional information
kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
kinetics of mutant enzymes, overview
-
additional information
additional information
-
kinetics of mutant enzymes, overview
-
additional information
additional information
-
kinetics do not fit a Michaelis-Menten equation
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
FJ560721
steady-state kinetics, overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics, overview
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics, overview
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics, overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics, overview
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10
2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
45°C, pH 6.0, LccA protein isolated from Haloferax volcanii H26 strain US02
29
bilirubin
45°C, pH 8.4, LccA protein isolated from Haloferax volcanii H26 strain US02
15.5
1-hydroxybenzotriazole
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
28.8
1-hydroxybenzotriazole
pH 5.0, temperature not specified in the publication, engineered enzyme 7D5
3.56
1-naphthol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
3.89
1-naphthol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
6.57
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae
7.2
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, mutant I494A
18.2
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, recombinant wild-type enzyme
28.1
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 25°C, recombinant wild-type enzyme
36.5
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, recombinant mutant WlacS
48.6
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 22°C, deglycosylated enzyme
50.9
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, recombinant mutant WlacD
52
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, mutant L386A
59.3
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
pH 4.5, 22°C, native enzyme
322
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
pH 4.0, 25°C, wild-type CotA
286
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate)
-
pH 3.0, 25°C, laccase Lac-3.5
409
2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonate)
-
pH 3.0, 25°C, laccase Lac-4.8
0.0046
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3, temperature not specified in the publication, mutant enzyme V159E/N398D
0.3
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
70°C, pH 3.0
0.431
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH not specified in the publication
0.696
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH not specified in the publication
6
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme H165A/R240H, 30°C, pH not specified in the publication
6
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme H165A/R240H
9
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme H165A/M199G, 30°C, pH not specified in the publication
9
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme H165A/M199G
15
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
wild-type enzyme, 30°C, pH not specified in the publication
15
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, wild-type enzyme
20
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199G/R240H, 30°C, pH not specified in the publication
20
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme M199G/R240H
23.1
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 30°C
29
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme Y230A, 30°C, pH not specified in the publication
29
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme Y230A
33
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199A, 30°C, pH not specified in the publication
33
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3, temperature not specified in the publication, mutant enzyme V159E
33
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, mutant enzyme M199A
34.39
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
42.6
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 30°C
44.4
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, temperature not specified in the publication, wild-type PM1 laccase
45
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199G, 30°C, pH not specified in the publication
45
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, mutant enzyme M199G
46.94
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
198.29
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, temperature not specified in the publication
240
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, temperature not specified in the publication, engineered enzyme 7D5
315
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C
409
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C
0.14
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
FJ560721
pH 5.0, 22-25°C
0.6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
1.15
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
4.22
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 30°C
4.26
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant K532A
4.58
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant P530A
5.51
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant K532E
7.67
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
10.1
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C
15.93
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
38
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
60.6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant P530A
65.6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant enzyme, pH 3.0, 30°C
70.02
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant K532A
70.6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant P530A
74.67
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant K532E
97.85
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant K532E
100.7
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant K532A
107.1
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
at 30°C in 100 mM sodium tartrate buffer (pH 4.5)
109
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
127
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
at 22°C, pH not specified in the publication
144.6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
196
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C
291
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 22°C, recombinant enzyme
594
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, temperature not specified in the publication
934.6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 50°C
1480
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
in 67 mM sodium acetate buffer (pH 4.5), at 30°C
7109
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.0, 50°C
0.012988
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
laccase DcLac1, pH 2.8, temperature not specified in the publication
0.018534
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
laccase DcLac2, pH 2.8, temperature not specified in the publication
0.058
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 7.0 37°C
1.02
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 35°C
1.467
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, isoenzyme Lac4
1.9
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, temperature not specified in the publication
3.485
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, isoenzyme Lac3
6.45
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
6.85
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH and temperature not specified in the publication
7.7
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
25°C, pH 4.0
15.7
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
21.2
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188A, in aqueous solution
23
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C, recombinant enzyme
31.11
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188K, in aqueous solution
32.03
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 37°C
32.8
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 4.5
42.2
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, wild-type enzyme
45.8
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, wild-type enzyme, in aqueous solution
67.04
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 8.0, 85°C
69.7
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
72
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.4, 70°C
86.4
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188L, in aqueous solution
98.2
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188R, in aqueous solution
99.5
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188V, in aqueous solution
102.8
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188I, in aqueous solution
309
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 22°C
377
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 30°C
399
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4, 30°C
2468
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH and temperature not specified in the publication
1.58
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
50°C, pH 6.0
3.33
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
55°C, pH 6.0
4.77
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
60°C, pH 6.0
24.6
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
pH and temperature not specified in the publication
0.064
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 505 kDa
0.08
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 214 kDa
0.101
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 432 kDa
0.168
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
enzyme conjugated with chitosan of 131 kDa
0.252
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
free enzyme
0.26
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.6, 37°C, mutant M502F
1.3
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant wild-type enzyme
1.6
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant mutant D206A
3.3
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant mutant D206E
4.3
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C, recombinant mutant D206N
10.3
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 5.5, 20°C
10.6
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.6, 37°C, mutant M502L
11.7
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.0, 25°C
22.4
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 7.6, 37°C, wild-type enzyme
68.87
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3.0, 25°C
182
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Fomes sclerodermeus
-
pH 5.0, isozyme Lac II
199
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
Fomes sclerodermeus
-
pH 5.0, isozyme Lac I
204
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.2, 45°C
272
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 4.5, 65°C
1520
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 4.5, 40°C
73300
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
25°C, isozyme POXA3a
158300
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
-
25°C, isozyme POXA3b
0.15
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant M456A, presence of 0.5 mM Cu2+, 30°C, pH 5
0.16
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant M455L, 30°C, pH 5
0.25
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, 30°C, pH 5
0.58
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
G2H480
pH 7.0, 25°C
0.69
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, presence of 0.5 mM Cu2+, 30°C, pH 5
0.98
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, pH 5.5, 25°C
1.1
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant P444A/M510Q, pH 5.5, temperature not specified in the publication
5.7
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant P444I, pH 5.5, 25°C
8.7
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant P444A, pH 5.5, 25°C
10
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant P444L, pH 5.5, 25°C
15
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion plus D439A/P444A mutant, pH 5.5, 25°C
17
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant D439A, pH 5.5, 25°C
17
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion plus P444A mutant, pH 5.5, 25°C
23
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion plus P444A mutant, pH 5.5, 25°C
38
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion mutant, pH 5.5, 25°C
40
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant D439A/P444A, pH 5.5, 25°C
50
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion plus P444G mutant, pH 5.5, 25°C
137
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant D439A/M510L, pH 5.5, temperature not specified in the publication
147
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
region Pro357-His406 deletion plus D439A mutant, pH 5.5, 25°C
2
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C
2.5
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
recombinant wild-type enzyme MmPPOA-695, pH 5.0, 25°C
4.5
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)
native wild-type enzyme, pH 5.0, 25°C
67
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA))
170
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, C-6His tagged laccase 3
299
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H)
328
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)), 6His tagged (C(DELTA)6H) and hyper-glycosylated
445
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, deglycosylated enzyme
549
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, wild-type enzyme
2150
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 6.0, 40°C
3 - 6
2,5-diaminotoluene
mutant M510L, pH 5.5, temperature not specified in the publication
3.2
2,5-diaminotoluene
mutant D439A/M510Q, pH 5.5, temperature not specified in the publication
11
2,5-diaminotoluene
mutant P444A/M510Q, pH 5.5, temperature not specified in the publication
16
2,5-diaminotoluene
mutant D439A/P444A/M510Q, pH 5.5, temperature not specified in the publication
19
2,5-diaminotoluene
wild-type, pH 5.5, temperature not specified in the publication
25
2,5-diaminotoluene
mutant P444A/M510Q, pH 5.5, temperature not specified in the publication
105
2,5-diaminotoluene
mutant P444A, pH 5.5, temperature not specified in the publication
233
2,5-diaminotoluene
mutant D439A/P444A, pH 5.5, temperature not specified in the publication
366
2,5-diaminotoluene
mutant D439A, pH 5.5, temperature not specified in the publication
832
2,5-diaminotoluene
mutant D439A/M510L, pH 5.5, temperature not specified in the publication
0.08
2,6-dimethoxyphenol
-
pH 5, temperature not specified in the publication, mutant enzyme V159E/N398D
0.1
2,6-dimethoxyphenol
recombinant mutant enzyme MmPPOA-637, pH 5.0, 25°C
0.11
2,6-dimethoxyphenol
mutant enzyme H165A/R240H, 30°C, pH not specified in the publication
0.11
2,6-dimethoxyphenol
30°C, pH 9.0, mutant enzyme H165A/R240H
0.178
2,6-dimethoxyphenol
30°C, pH not specified in the publication
0.21
2,6-dimethoxyphenol
recombinant His-tagged mutant enzyme MmPPOA-637, pH 5.0, 25°C
0.31
2,6-dimethoxyphenol
mutant enzyme H165A/M199G, 30°C, pH not specified in the publication
0.31
2,6-dimethoxyphenol
30°C, pH 6.5, mutant enzyme H165A/M199G
0.35
2,6-dimethoxyphenol
wild-type enzyme, 30°C, pH not specified in the publication
0.51
2,6-dimethoxyphenol
pH 3.4, 30°C, recombinant wild-type enzyme
0.92
2,6-dimethoxyphenol
recombinant wild-type enzyme MmPPOA-695, pH 5.0, 25°C
1.3
2,6-dimethoxyphenol
mutant enzyme M199A, 30°C, pH not specified in the publication
1.3
2,6-dimethoxyphenol
30°C, pH 8.0, mutant enzyme M199A
1.68
2,6-dimethoxyphenol
mutant enzyme Y230A, 30°C, pH not specified in the publication
1.68
2,6-dimethoxyphenol
30°C, pH 8.5, mutant enzyme Y230A
1.74
2,6-dimethoxyphenol
recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C
1.98
2,6-dimethoxyphenol
native wild-type enzyme, pH 5.0, 25°C
2.441
2,6-dimethoxyphenol
30°C, pH not specified in the publication
3
2,6-dimethoxyphenol
-
at 22°C, pH not specified in the publication
3.39
2,6-dimethoxyphenol
mutant enzyme M199G, 30°C, pH not specified in the publication
3.39
2,6-dimethoxyphenol
30°C, pH 8.0, mutant enzyme M199G
4.18
2,6-dimethoxyphenol
mutant enzyme M199G/R240H, 30°C, pH not specified in the publication
4.18
2,6-dimethoxyphenol
30°C, pH 8.5, mutant enzyme M199G/R240H
6
2,6-dimethoxyphenol
-
pH 5, temperature not specified in the publication, mutant enzyme V159E
6.85
2,6-dimethoxyphenol
-
pH and temperature not specified in the publication
9.08
2,6-dimethoxyphenol
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae
9.43
2,6-dimethoxyphenol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
10.2
2,6-dimethoxyphenol
pH 4.5, 25°C, recombinant wild-type enzyme
11.23
2,6-dimethoxyphenol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
13.2
2,6-dimethoxyphenol
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
16.3
2,6-dimethoxyphenol
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
23.2
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, wild-type enzyme
26.4
2,6-dimethoxyphenol
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
34.4
2,6-dimethoxyphenol
pH 8.5, temperature not specified in the publication
38.2
2,6-dimethoxyphenol
-
pH 5.0, temperature not specified in the publication
38.8
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
45.3
2,6-dimethoxyphenol
pH 5.0, temperature not specified in the publication, engineered enzyme 7D5
68
2,6-dimethoxyphenol
-
pH and temperature not specified in the publication
80.4
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
703.7
2,6-dimethoxyphenol
pH and temperature not specified in the publication
0.32
4-hydroxy-3,5-dimethoxybenzaldehyde azine
pH 5.0, 25°C
6.85
4-hydroxy-3,5-dimethoxybenzaldehyde azine
-
pH and temperature not specified in the publication
3.02
4-hydroxybenzoic acid
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
8.6
4-hydroxybenzoic acid
pH 5.0, temperature not specified in the publication, engineered enzyme 7D5
0.084
catechol
recombinant mutant enzyme MmPPOA-637, pH 5.0, 25°C
1.53
catechol
recombinant wild-type enzyme MmPPOA-695, pH 5.0, 25°C
2.18
catechol
recombinant His-tagged enzyme MmPPOA-695, pH 5.0, 25°C
1.34
guaiacol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
1.57
guaiacol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
2.85
guaiacol
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
8
guaiacol
pH 8.5, temperature not specified in the publication
10.5
guaiacol
pH 5.0, temperature not specified in the publication, engineered enzyme 7D5
39.5
guaiacol
22°C, pH 7.0, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
64.9
guaiacol
22°C, pH 7.0, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
0.2
K4[Fe(CN)6]
pH 4.0, temperature not specified in the publication
1.7
N,N-dimethyl-1,4-phenylenediamine
-
pH 4, temperature not specified in the publication, mutant enzyme V159E/N398D
56
N,N-dimethyl-1,4-phenylenediamine
-
pH 4, temperature not specified in the publication, mutant enzyme V159E
108.6
N,N-Dimethyl-p-phenylenediamine
pH 4.0, temperature not specified in the publication, wild-type PM1 laccase
938
N,N-Dimethyl-p-phenylenediamine
pH 4.0, temperature not specified in the publication, engineered enzyme 7D5
1.33
N-acetyldopamine
pH 5.0-5.5, recombinant N-terminally truncated enzyme
0.057
o-Dianisidine
30°C, pH not specified in the publication
0.748
o-Dianisidine
30°C, pH not specified in the publication
21.6
Sinapic acid
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
52.8
Sinapic acid
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
0.365
syringaldazine
30°C, pH not specified in the publication
0.573
syringaldazine
30°C, pH not specified in the publication
6.47
syringaldazine
pH and temperature not specified in the publication
20
syringaldazine
-
at 22°C, pH not specified in the publication
21.05
syringaldazine
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae
22
syringaldazine
45°C, pH 6.0, LccA protein isolated from Haloferax volcanii H26 strain US02
40.17
syringaldazine
pH 4.5, 25°C, recombinant wild-type enzyme
71
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA))
188
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H)
246
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H) and hyper-glycosylated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
15
2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
45°C, pH 6.0, LccA protein isolated from Haloferax volcanii H26 strain US02
123
bilirubin
45°C, pH 8.4, LccA protein isolated from Haloferax volcanii H26 strain US02
0.33
1-hydroxybenzotriazole
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
0.84
1-hydroxybenzotriazole
pH 5.0, temperature not specified in the publication
0.64
1-naphthol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
0.64
1-naphthol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.076
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
70°C, pH 3.0
1.72
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH and temperature not specified in the publication
4.331
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH not specified in the publication
15.6
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C
19.03
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 30°C
29.245
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH not specified in the publication
42
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
wild-type enzyme, 30°C, pH not specified in the publication
42
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, wild-type enzyme
74
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme H165A/R240H, 30°C, pH not specified in the publication
74
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme H165A/R240H
95
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme H165A/M199G, 30°C, pH not specified in the publication
95
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme H165A/M199G
102
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199A, 30°C, pH not specified in the publication
102
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, mutant enzyme M199A
157.86
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH and temperature not specified in the publication
160
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
169
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme Y230A, 30°C, pH not specified in the publication
169
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme Y230A
203
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199G/R240H, 30°C, pH not specified in the publication
203
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 3.5, mutant enzyme M199G/R240H
226
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
mutant enzyme M199G, 30°C, pH not specified in the publication
226
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
30°C, pH 4.0, mutant enzyme M199G
455
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, 30°C
620
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
6880
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, 30°C
7391
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3, temperature not specified in the publication, mutant enzyme V159E
9914.5
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 5.0, temperature not specified in the publication
26106
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, temperature not specified in the publication, wild-type PM1 laccase
75500
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
pH 3, temperature not specified in the publication, mutant enzyme V159E/N398D
184500
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.0, temperature not specified in the publication
0.0552
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 6.0, 25°C
0.074
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant P530A
0.133
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant K532E
0.15
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
0.154
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 6.5, 25°C, mutant K532A
0.672
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant P530A
1.165
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant K532E
1.73
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 25°C, mutant K532A
1.78
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
2.981
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant P530A
3.505
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant K532E
5.05
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
FJ560721
pH 5.0, 22-25°C
6.434
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 25°C, mutant K532A
42.55
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
49.1
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 3.0, 30°C
222
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C
380
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
465.8
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, recombinant Lac1
630
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant enzyme, pH 3.0, 30°C
1024
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
1085
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
1300
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
at 30°C in 100 mM sodium tartrate buffer (pH 4.5)
3960
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, temperature not specified in the publication
4780
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
pH 5.0, 25°C
5164
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
643500
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
in 67 mM sodium acetate buffer (pH 4.5), at 30°C
0.00333
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
laccase DcLac1, pH 2.8, temperature not specified in the publication
0.01477
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
laccase DcLac2, pH 2.8, temperature not specified in the publication
0.19
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 35°C
0.42
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, temperature not specified in the publication
7.167
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, isoenzyme Lac4
7.61
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
17.6
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 25°C
25.625
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.5, 40°C, isoenzyme Lac3
47.82
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 8.0, 85°C
59.6
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
118.28
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 7.0 37°C
120
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188A, in aqueous solution
142.1
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 4.5
163.1
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH and temperature not specified in the publication
210
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188K, in aqueous solution
322
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, wild-type enzyme, in aqueous solution
383
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
21-23°C, pH not specified in the publication, wild-type enzyme
670
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188L, in aqueous solution
750
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188V, in aqueous solution
890
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188I, in aqueous solution
900
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4.0, 22°C, mutant enzyme E188R, in aqueous solution
1596
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH 4, 30°C
2328
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 5.0, 22°C
2609
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 3.4, 70°C
2642
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
pH and temperature not specified in the publication
35519
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
-
pH 4.0, 25°C
0.065
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant M456A, presence of 0.5 mM Cu2+, 30°C, pH 5
0.085
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, 30°C, pH 5
0.437
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
wild-type, presence of 0.5 mM Cu2+, 30°C, pH 5
0.521
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate)
mutant M455L, 30°C, pH 5
70
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA))
240
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, deglycosylated enzyme
240
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H)
260
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)), 6His tagged (C(DELTA)6H) and hyper-glycosylated
270
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, wild-type enzyme
530
2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30°C, pH 6.0, C-6His tagged laccase 3
0.74
2,6-dimethoxyphenol
mutant enzyme H165A/R240H, 30°C, pH not specified in the publication
0.74
2,6-dimethoxyphenol
30°C, pH 9.0, mutant enzyme H165A/R240H
1.09
2,6-dimethoxyphenol
wild-type enzyme, 30°C, pH not specified in the publication
1.11
2,6-dimethoxyphenol
mutant enzyme H165A/M199G, 30°C, pH not specified in the publication
1.11
2,6-dimethoxyphenol
30°C, pH 6.5, mutant enzyme H165A/M199G
1.55
2,6-dimethoxyphenol
mutant enzyme M199A, 30°C, pH not specified in the publication
1.55
2,6-dimethoxyphenol
30°C, pH 8.0, mutant enzyme M199A
2.22
2,6-dimethoxyphenol
mutant enzyme Y230A, 30°C, pH not specified in the publication
2.22
2,6-dimethoxyphenol
30°C, pH 8.5, mutant enzyme Y230A
5.07
2,6-dimethoxyphenol
mutant enzyme M199G, 30°C, pH not specified in the publication
5.07
2,6-dimethoxyphenol
30°C, pH 8.0, mutant enzyme M199G
9.102
2,6-dimethoxyphenol
30°C, pH not specified in the publication
14.4
2,6-dimethoxyphenol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
16.51
2,6-dimethoxyphenol
mutant enzyme M199G/R240H, 30°C, pH not specified in the publication
16.51
2,6-dimethoxyphenol
30°C, pH 8.5, mutant enzyme M199G/R240H
20
2,6-dimethoxyphenol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
21.2
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
24.532
2,6-dimethoxyphenol
30°C, pH not specified in the publication
37.2
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
42.8
2,6-dimethoxyphenol
-
pH and temperature not specified in the publication
57
2,6-dimethoxyphenol
21-23°C, pH not specified in the publication, wild-type enzyme
75.9
2,6-dimethoxyphenol
-
pH 5.0, temperature not specified in the publication
81
2,6-dimethoxyphenol
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
90.5
2,6-dimethoxyphenol
pH 8.5, temperature not specified in the publication
113
2,6-dimethoxyphenol
-
pH and temperature not specified in the publication
371
2,6-dimethoxyphenol
-
pH 5, temperature not specified in the publication, mutant enzyme V159E
581
2,6-dimethoxyphenol
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
905
2,6-dimethoxyphenol
pH 5.0, temperature not specified in the publication
1325
2,6-dimethoxyphenol
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
1490
2,6-dimethoxyphenol
-
pH 5, temperature not specified in the publication, mutant enzyme V159E/N398D
3500
2,6-dimethoxyphenol
pH and temperature not specified in the publication
14000
2,6-dimethoxyphenol
-
at 30°C in 100 mM sodium tartrate buffer (pH 4.5)
74.4
4-hydroxy-3,5-dimethoxybenzaldehyde azine
-
pH and temperature not specified in the publication
1.54
4-hydroxybenzoic acid
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
6.1
4-hydroxybenzoic acid
pH 5.0, temperature not specified in the publication
0.27
guaiacol
E1ACR6
pH and temperature not specified in the publication, wild-type enzyme
0.37
guaiacol
E1ACR6
pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
5.17
guaiacol
pH and temperature not specified in the publication
11.9
guaiacol
pH 5.0, temperature not specified in the publication, wild-type PM1 laccase
26.6
guaiacol
pH 8.5, temperature not specified in the publication
28.5
guaiacol
22°C, pH 7.0, mutant enzyme L386Q/G417I/V482G (variant L9 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I/V482G substitutions)
271
guaiacol
22°C, pH 7.0, mutant enzyme L386Q/G417I (variant L2 harbours the insertion of two amino acids (Ser and Pro) after the N-terminal methionine, in addition to the L386Q/G417I substitutions)
0.02
K4[Fe(CN)6]
pH 4.0, temperature not specified in the publication
107
N,N-dimethyl-1,4-phenylenediamine
-
pH 4, temperature not specified in the publication, mutant enzyme V159E
388
N,N-dimethyl-1,4-phenylenediamine
-
pH 4, temperature not specified in the publication, mutant enzyme V159E/N398D
102
N,N-Dimethyl-p-phenylenediamine
pH 4.0, temperature not specified in the publication, wild-type PM1 laccase
3253
N,N-Dimethyl-p-phenylenediamine
pH 4.0, temperature not specified in the publication
0.089
o-Dianisidine
30°C, pH not specified in the publication
5.187
o-Dianisidine
30°C, pH not specified in the publication
621
Sinapic acid
recombinant wild-type enzyme, pH 3.0, temperature not specified in the publication
1662
Sinapic acid
recombinant wild-type enzyme, pH 5.0, temperature not specified in the publication
5.672
syringaldazine
30°C, pH not specified in the publication
82.936
syringaldazine
30°C, pH not specified in the publication
628
syringaldazine
45°C, pH 6.0, LccA protein isolated from Haloferax volcanii H26 strain US02
3000
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA))
20000
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H) and hyper-glycosylated
23000
syringaldazine
30°C, pH 6.0, a variant of laccase 3 deleted of the last 13 residues (C(DELTA)) and 6His tagged (C(DELTA)6H)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0079
NaN3
pH 4.5, 25°C, recombinant wild-type enzyme versus 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
0.029
NaN3
pH 4.5, 25°C, recombinant wild-type enzyme, versus 2,6-dimethoxyphenol
0.055
NaN3
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae, versus 2,6-dimethoxyphenol
0.085
NaN3
pH 4.5, 25°C, recombinant mutant L559A expressed in Saccharomyces cerevisiae versus 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
additional information
additional information
-
inhibition kinetics with acidic phenols, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.7
SDS
Agrocybe pediades
-
pH and temperature not specified in the publication, mutant enzyme V159E/N398D
8
EDTA
Streptomyces viridochromogenes
pH 8.5, temperature not specified in the publication
14
EDTA
Streptomyces viridochromogenes
pH 4.0, temperature not specified in the publication
90
EDTA
Agrocybe pediades
-
pH and temperature not specified in the publication, mutant enzyme V159E/N398D
74
NaCl
Agrocybe pediades
-
pH and temperature not specified in the publication, mutant enzyme V159E/N398D
0.051
NaF
Agrocybe pediades
-
pH and temperature not specified in the publication, mutant enzyme V159E/N398D
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.089
E1ACR6
substrate: 1-naphthol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.13
E1ACR6
substrate: 1-naphthol, pH and temperature not specified in the publication, wild-type enzyme
0.45
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, pH 5.5, 25°C
0.573
-
Resource Q, purification of Pseudomonas putida F6 laccase-type activity
0.64
E1ACR6
substrate: guaiacol, pH and temperature not specified in the publication, wild-type enzyme
0.85
-
culture filtrate, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
1.12
E1ACR6
substrate: 2,6-dimethoxyphenol, pH and temperature not specified in the publication, wild-type enzyme
1.34
purified recombinant enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 25°C
1.37
E1ACR6
substrate: guaiacol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
1.41
1.52
-
recombinant enzyme from Escherichia coli AH3517 cells grown under aerobic conditions
100
partially purified recombinant truncated mutant POXA1bDELTA4, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
112.6
-
pH 4.0, 60°C, purified enzyme, substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
12
129
partially purified recombinant truncated mutant POXA1bDELTA16, substrate 2,6-dimethoxyphenol
13
recombinant enzyme, substrate: syringaldazine, pH 6.5, 37°C, recombinant enzyme
13.17
-
pH and temperature not specified in the publication, crude enzyme
13.5
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.0
148
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.0, 25°C
15
155
partially purified recombinant wild-type enzyme, substrate 2,6-dimethoxyphenol
16
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
162
partially purified recombinant wild-type enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
17
18.4
-
after 21.6fold purification, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
185.4
30°C, pH 4.5, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
189
partially purified recombinant truncated mutant POXA1bDELTA4, substrate 2,6-dimethoxyphenol
19
19.1
-
crude enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C
2.67
E1ACR6
substrate: 2,6-dimethoxyphenol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
20
22.8
E1ACR6
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH and temperature not specified in the publication, wild-type enzyme
228.2
-
recombinant enzyme from Escherichia coli AH3517 cells grown under microaerobic conditions
24 - 36.6
recombinant enzyme expressed in Saccharomyces cerevisiae
261
-
purified enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C
28.8
pH 4.5, 55°C, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
29
substrate 2,6-dimethoxyphenol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
3
partially purified recombinant truncated mutant POXA1bD205R, substrate 2,6-dimethoxyphenol
30
substrate 2,6-dimethoxyphenol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
3138
pH and temperature not specified in the publication, laccase activity in the culture supernatant
33
34
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
4
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), pH 5, 30°C
4.02
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4.18
purified native enzyme, substrate N-beta-alanyldopamine-alanyldopamine
4.57
-
gel filtration, purification of Pseudomonas putida F6 laccase-type activity
4.6
substrate p-phenylendiamine, region Pro357-His406 deletion mutant, pH 5.5, 25°C
460
purified enzyme, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
52
5778
60.8
-
purified native extracellular enzyme, pH 5.0, temperature not specified in the publication, substrate 2,6-dimethoxyphenol
69
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
79.8
E1ACR6
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
8
-
purified recombinant enzyme, with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid at pH 4.0
83.47
-
pH and temperature not specified in the publication, purified enzyme
850
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 4.5
86.5
additional information
1.41
Loweporus lividus
-
after 67.6fold purification, in 50 mM sodium malonate buffer, pH 4.5 at 37°C
12
substrate catechol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
12
partially purified recombinant truncated mutant POXA1bDELTA16, substrate syringaldazine
15
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, pH 5.5, 25°C
15
partially purified recombinant wild-type enzyme, substrate syringaldazine
17
partially purified recombinant truncated mutant POXA1bDELTA4, substrate syringaldazine
19
substrate p-phenylendiamine, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
20
substrate p-phenylendiamine, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
33
partially purified recombinant truncated mutant POXA1bDELTA16, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
33
partially purified recombinant truncated mutant POXA1bD205R, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
52
substrate catechol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
additional information
-
the specific activity of the crude enzyme from culture broth with small amount of brown impurities is 451 units/mg and the specific activity of the 11fold purified enzyme is 5111 units/mg. The specific activity of the crude enzyme from culture broth with big amount of brown impurities is 410 units/mg and the specific activity of the 11.38fold purified enzyme is 4666 units/mg. One unit of proteolytic activity is defined as the amount of enzyme that induces absorbance increase of 1.0 per 10 min at 37°C
additional information
-
the specific activity of the crude enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 11.3 units/mg, the specific activity of the 131fold purification enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 1480 units/mg. One unit of enzyme activity is defined as the amount of enzyme required to produce an absorbance increase at 420 nm of 1 per minute per ml of reaction mixture
additional information
-
relative activity of laccase in conjugation with chitosans of different molecular weights, overview
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
2.2
2.5
2.5 - 3
2.5 - 5.5
the optimal pH values for the different substrates 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62 oxidation are pH 2.5, pH 4.5, pH 5.0, pH 5.5 and pH 5.5, respectively
2.6
3 - 3.5
3 - 3.6
-
using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
3 - 5
3 - 6.5
3.2
-
assay at, with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
3.4
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
3.5
3.6
3.8 - 4.8
4 - 6
4 - 7
4.2
4.4
4.5
4.5 - 5
4.5 - 5.5
maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine
4.6
4.8
5 - 6
-
copper alginate and calcium alginate-chitosan immobilized laccase
5.5
5.6
6 - 7
6.2
6.5
6.6
6.8
7.4
7.5
7.6
8.5
additional information
2
-
or below, substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
2
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
2
wild-type enzyme with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
2
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
2.2
Phlebia fascicularia
-
Lac I and Lac II, substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
2.5
subatrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
2.5
-
both isoenzymes, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
2.5
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
3
-
or less, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
3
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol
3
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
3
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
3
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1
3
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac3
3
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac4
3
pH-optima below 3, substrates: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,6-dimethoxyphenol
3
-
substrates 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, catechol, pyrogallol
3
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
3
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
3
below, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
3
substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol
3
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol
3
substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonix acid) or 2,6-dimethoxyphenol
3.5
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
3.5
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
3.5
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzyme H165A/M199G, mutant enzyme M199G/R240H, mutant enzyme H165A/R240H
3.5
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzymes Y230A, H165A/M199G, M199G/R240H and H165A/R240H
3.5
substrate: guaiacol
3.6
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
3.6
-
POXA3a and POXA3b, substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
4
-
substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, wild-type enzyme and mutant enzymes
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
-
substrates guaiacol, beta-(3,4-dihydroxyphenyl)alanine, p-phenylendiamine
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
Oscillatoria subuliformis
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), wild-type enzyme, mutant enzyme M199A, mutant enzyme M199G and mutant enzyme Y230A
4
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), wild-type enzyme, mutant enzyme M199A and mutant enzyme M199G. Substrate: K4[Fe(CN)6], wil-type enzyme, and mutant enzymes M199A, M199G, Y230A, H165A/M199G, M199G/R240H and H165A/R240H
4
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
4.2
-
substrate: 2,2-azino-bis-(3-ethylbenzthiazolin-6-sulfonate) or 2,6-dimethoxyphenol
4.4
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
4.5
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
4.5
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4.8
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
4.8
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
5
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
5
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
5
FJ560721
with substrates 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 2,6-dimethoxyphenol
5.5
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
5.5
substrate: syringaldazine
6
-
substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
6
-
substrates guaiacol, 3-dimethylaminobenzoic acid, 3-methyl-2-benzothiazolinone hydrazone
6
-
non-phenolic substrate: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
6
with substrates guaiacol and syringaldazine, recombinant Lac1
6
oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol
6
optimal oxidation of 2,2,-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
6.5
substrate: 2,6-dimethoxyphenol, mutant enzyme H165A/M199G
7
-
substrate 2,6-dimethoxyphenol, assay at for the wild-type enzyme and the L386A mutant
8
substrate: 2,6-dimethoxyphenol, mutant enzyme M199G and mutant enzyme M199A
8
substrate: 2,6-dimethoxyphenol, mutant enzymes M199A and M199G
8.5
substrate: 2,6-dimethoxyphenol, mutant enzyme Y230A and mutant enzyme M199G/R240H,
8.5
substrate: 2,6-dimethoxyphenol, mutant enzyme Y230A and M199G/R240H
9
substrate: 2,6-dimethoxyphenol, wild-type enzyme and mutant enzyme H165A/R240H
additional information
-
the pH optima of the isozymes with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid are in the range of pH 1.2-2.0
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2 - 10
Streptomyces sp. LAO
MK290990.1
pH 2.0: about 70% of maximal activity, pH 10.0: about 80% of maximal activity
2 - 11
for pH ranges between pH 2 and pH 11 enzyme activity ranges between 75 and 100%
2 - 12
-
the purified laccase maintains stable at pH 3.0-11.0. The enzyme remains active at pH values from 3.0 to 12.0, and its activity at pH 2.0 and 12.0 are 56.6% and 85.5% of that at pH 7.0, respectively
2 - 5
2 - 5.5
Myrioconium sp.
-
pH 2.0: about 65% of maximal activity, pH 5.5: about 45% of maximal activity, oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate)
2 - 6
2 - 8
2.2 - 4.2
2.2 - 4.3
-
pH 2.2: about 90% of maximal activity, pH 4.3: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
2.3 - 4
pH 2.3: about 40% of maximal activity, pH 4.0: about 75% of maximal activity
2.5 - 5
2.5 - 5.5
-
inactivation of isozyme Lacc I at pH 6.5, of isozyme Lacc II at pH 7.5
3 - 10
3 - 4
3 - 6
3 - 6.5
3 - 7
3 - 8
3 - 9
recombinant wild-type enzyme, active range and broad optimum
3.5 - 4.5
-
pH 3.5: about 55% of maximal activity, pH 4.5: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
3.5 - 5
-
pH 3.5: about 70% of maximal activity, pH 5.0: about 70% of maximal activity
3.5 - 5.5
4 - 10
-
activity range, 60-65% of maximal activity at pH 4.0 and pH 10.0, profile, overview
4 - 5.5
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
4 - 6
-
pH 4.0: about 70% of maximal activity, pH 6.0: about 85% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
4 - 7
pH 4.0: about 35% of maximal activity, pH 7.0: about 55% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
4 - 8
4 - 9
4.5 - 7.3
5 - 8
5 - 8.5
-
pH 5.0: about 50% of maximal activity, pH 8.5: about 50% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
5.5 - 6.5
Myrioconium sp.
-
pH 5.5: about 90% of maximal activity, pH 6.5: about 60% of maximal activity, oxidation of syringaldazine
5.5 - 8.5
-
pH 5.5: about 75% of maximal activity, pH 8.5: about 50% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
6 - 7.5
6 - 8
6 - 9
7.5 - 8.5
-
pH 7.5: about 45% of maximal activity, pH 8.5: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol
7.5 - 9
-
pH 7.5: about 65% of maximal activity, pH 9.0: 72% of maximal activity
8 - 11.5
-
retains more than 80% of activity after 17 h in this pH-range
additional information
2 - 5
-
pH 2.0: about 75% of maximal activity, pH 5.0: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
2 - 6
pH 2.0: about 30% of maximal activity, pH 6.0: about 30% of maximal activity
2 - 8
-
the activity rises abruptly when the pH is increased from slightly below 2.0. The activity declines sharply when the pH is raised from 4.0 to 5.0 and is indiscernible at pH 6.0-8.0
2.2 - 4.2
-
pH 2.2: about 40% of maximal activity, pH 4.2: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
2.2 - 4.2
-
pH 2.2: about 40% of maximal activity, pH 4.2: about 60% of maximal activity, substrate: 2,6-dimethoxyphenol
2.5 - 5
-
pH 2.5: about 85% of maximal activity, pH 5.0: about 50% of maximal activity, oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
3 - 4
-
pH 3.0: about 40% of maximal activity, pH 4.0: about 30% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
3 - 6
-
pH 3.0: about 60% of maximal activity, pH 6.0: about 55% of maximal activity
3 - 6
Loweporus lividus MTCC-1178
-
pH 3.0: about 45% of maximal activity, pH 6.0: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol
3 - 8
recombinant mutant enzyme MmPPOA-637, inactivation at pH 8.0 and above
3 - 8
pH 3.0: about 75% of maximal activity, pH 8.0: about 60% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
3.5 - 5.5
Loweporus lividus
-
the enzyme retains more than 50% of its maximum activity in the pH range of 3.5-5.5
3.5 - 5.5
-
pH 3.5: about 70% of maximal activity, pH 5.5: about 60% of maximal activity, substrate: guaiacol
4 - 8
E1ACR6
pH 4.0: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, wild-type enzyme
4 - 8
E1ACR6
pH 4.0: about 90% of maximal activity, pH 8.0: about 50% of maximal activity, mutant enzyme V281A/P309L/S318G/D232V
4 - 9
-
pH 4.0: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
5 - 8
activity range, at pH 7.0, about 17%, 24%, 33%, 23% and 21% of the maximum oxidation rate is observed for substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62, respectively, while approximately 2%, 8%, 15%, 4% and 17% of the maximum activity in oxidation of these substrates is obtained at pH 8.0
5 - 8
-
pH 5.0: about 20% of maximal activity, pH 8.0: about 65% of maximal activity. The enzyme is inactive at pH 4.0 and at pH 9.0
6 - 7.5
pH 6.0: about 50% of maximal activity, pH 7.5: abput 80% of maximal activity
6 - 7.5
-
pH 6.0: about 55% of maximal activity, pH 7.5: about 55% of maximal activity, immobilized enzyme, substrate: 2,6-dimethoxyphenol
6 - 8
-
pH 6.0: about 25% of maximal activity, pH 8.0: about 70% of maximal activity, substrate: 2,6-dimethoxyphenol
6 - 9
pH 6.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity, substrate: 2,6-dimethoxyphenol
6 - 9
-
pH 6.0: about 40% of maximal activity, pH 9.0: about 60% of maximal activity
additional information
pH dependencies of wild-type and mutant enzymes, overview
additional information
-
pH dependencies of wild-type and mutant enzymes, overview
additional information
phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH
additional information
-
phenolic compounds are oxidized in the presence of the enzyme under alkaline but not acidic conditions. Conversely, nonphenolic compounds are oxidized at acidic but not alkaline pH
additional information
-
pH profiles of free and chitosan-conjugated enzyme
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 30
-
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
22
23
25
30
35
37
40
45
50
50 - 60
55
60
65
70
75
80
85
90
92
at pH 5.0, maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate)
25
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
25
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
25
Oscillatoria subuliformis
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
25
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
25
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
25
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
25
-
assay at, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
40
-
substrates: 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine or 2,6-dimethoxyphenol
45
optimal oxidation of syringaldazine and 2,2,-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
50
-
with substrates 2,6-dimethoxyphenol, syringaldazine, and guaiacol
50
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
50
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
55
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or syringaldazine
55
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
60
subatrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
60
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac4
60
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzyme M199G
65
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), recombinant Lac1
65
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac3
70
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
70
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), mutant enzymes M199A and Y230A
75
above 75°C, both wild-type and region Pro357-His406 deletion mutant
80
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol
80
FJ560721
with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
80
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
80
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
80
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), wild-type enzyme
85
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0 - 100
-
a cold adaptation enzyme with a relative catalytic activity of 30.2% at 0°C, but also a high thermostable enzyme. About 50-70% of relative activity at 10-30°C
20 - 100
-
24% of maximal activity at 100°C, 65% at 20°C, activity profile, overview
20 - 50
20 - 60
20 - 70
-
20°C: about 60% of maximal activity, 70°C: about 50% of maximal activity
20 - 80
21 - 60
21°C: 75% of maximal activity, 60°C: 40-50% of maximal activity
25 - 60
25 - 65
the enzyme displays more than 50% of maximal activity between 25°C and 65°C against guaiacol, catechol or 2,6-dimethoxyphenol. At 65°C for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), the relative activity of LacA is 45%
25 - 70
25 - 80
25 - 95
-
25°C: about 50% of maximal activity, 95°C: about 40% of maximal activity
30 - 100
30 - 40
-
30°C: about 50% of maximal activity, 40°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30 - 60
-
30°C: about 55% of maximal activity, 65°C: about 55% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
30 - 70
30 - 80
30 - 90
35 - 60
35 - 65
37 - 100
-
37°C: about 55% of maximal activity, 100°C: about 60% of maximal activity, substrate: 2,6-dimethoxyphenol
40 - 70
40 - 75
maximum activity at 50°C, retaining more than 80% of activity at temperatures between 60°C and 75°C, but less than 55% of activity at temperatures below 40°C
40 - 80
40 - 85
40°C: about 40% of maximal activity, 85°C: about 55% of maximal activity
40 - 90
-
40°C: about 60% of maximalm activity, 90°C: about 75% of maximal activity
45 - 60
45 - 75
45°C: about 45% of maximal activity, 75°C: about 55% of maximal activity, substrate: syringaldazine
45 - 80
-
45°C: about 60% of maximal activity, 80°C: about 65% of maximal activity, substrate: 2,6-dimethoxyphenol
5 - 70
-
the enzyme activity increases by about 70% to its maximum at 30°C when the temperature is elevated from 5°C, and then falls to about 10% of the maximal value when the temperature is raised to 70°C
5 - 75
-
high activity at 5°C, 25 and 30% of maximum activity at 60°C for isozymes Lacc I and II, 20% at 75°C
50 - 70
-
all the forms of immobilized laccase show more than 90% relative activity at higher temperature of 55°C and 60°C. Sol-gel immobilized laccase shows 97% (65°C) and 94% (70°C) relative activity at higher temperatures. Soluble enzyme loses maximum activity above 55°C
50 - 80
50 - 90
-
rapid increase in laccase activity from 50°C to 80°C followed by a decline inlaccase activity of approximately 26% at 90°C
55 - 90
-
55°C: about 50% of maximal activity, 90°C: about 45% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
60 - 95
-
the recombinant LacG enzyme shows more than 80% activity within 60-95°C
65 - 100
-
65°C: about 50% of maximal activity, 100°C: about 50% of maximal activity
additional information
20 - 50
-
20°C: about 40% of maximal activity, 50°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
20 - 50
20°C: about 80% of maximal activity, 50°C: about 60% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
20 - 60
20°C: about 60% of maximal activity, 60°C: about 50% of maximal activity
20 - 60
-
20°C: about 50% of maximal activity, 60°C: about 55% of maximal activioty, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
20 - 80
-
20°C: about 70% of maximal activity, 80°C: about 65% of maximal activity, oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
20 - 80
20°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
20 - 80
20°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, substrate: 2,6-dimethoxyphenol
20 - 80
-
high activity at 80°C, optimum at 70°C, constant rise of activity from 20°C to 70°C
25 - 60
-
40% of maximal activity at 25°C, maximal activity at 45°C, 90% of maximal activity at 50°C, 85% of maximal activity at 55°C, and 69% at 60°C
25 - 70
-
25°C: about 55% of maximal activity, 75°C: about 75% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
25 - 70
25°C: about 75% of maximal activity, 70°C: about 55% of maximal activity
25 - 80
-
25°C: 29% of maximal activity, 80°C: about 70% of maximal activity
30 - 100
Streptomyces sp. LAO
MK290990.1
30°C: about 70% of maximal activity, 100°C: about 90% of maximal activity
30 - 70
optimum temperature of CcLCC5I is 55°C, the activity of laccase is high from 30 to 50°C, when the temperature is over 55°C, the activity declines more, when the temperature reaches 70°C, the relative activity of Lcc is only 23%, profile, overview
30 - 70
Myrioconium sp.
-
30°C: about 75% of maximal activity, 70°C: optimum, oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate)
30 - 70
-
30°C: about 30% of maximal activity, 70°C: about 50% of maximal activity
30 - 80
-
30°C: about 45% of maximal activity, 80°C: about 55% of maximal activity
30 - 80
30°C: about 50% of maximal activity, 80°C: about 45% of maximal activity
30 - 80
30°C: about 70% of maximal activity, 80°C: about 45% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
35 - 60
35°C: about 30% of maximal activity, 60°C: about 35% of maximal activity, substrate: syringaldazine
35 - 60
-
35°C: about 60% of maximal activity, 60°C: about 60% of maximal activity, immobilized enzyme, substrate: 2,6-dimethoxyphenol
35 - 65
E1ACR6
35°C: about 65% of maximal activity, 65°C: about 50% of maximal activity, wild-type enzyme
35 - 65
E1ACR6
35°C: about 65% of maximal activity, 65°C: about 75% of maximal activity, mutant enzyme V281A/P309L/S318G/D232V
40 - 70
Loweporus lividus MTCC-1178
-
40°C: about 35% of maximal activity, 70°C: about 35% of maximal activity, substrate: 2,6-dimethoxyphenol
40 - 70
-
40°C: about 50% of maximal activity, 70°C: about 90% of maximal activity
40 - 80
Loweporus lividus
-
the enzyme retains more than 50% of its maximum activity at temperature above 40°C
40 - 80
40°C: about 40% of maximal activity, 80°C: maximal activity, substrate: 2,6-dimethoxyphenol
45 - 60
45°C: about 20% of maximal activity, 60°C: about 25% of maximal activity
50 - 80
-
50°C: about 20% of maximal activity, 90°C: about 40% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
50 - 80
50°C: about 50% of maximal activity, 80°C: maximal activity, substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.3
3.5
3.55
recombinant MaL, expressed in Saccharomyces cerevisiae, has several pI forms, whereas the recombinant MaL, produced in Trichoderma reesei only had one pI form at pI 4.0
3.7
3.8
4.2
4.8
4.9
5.1
5.6
5.8
5.9
Lcc2 is comprised of 5 individual isoforms with pI values ranging from 3.5 to 5.8
6
Lcc2 is comprised of 5 individual isoforms with pI values ranging from 3.5 to 5.8
6.1
Lcc2 is comprised of 5 individual isoforms with pI values ranging from 3.5 to 5.8
5.8
Lcc2 is comprised of 5 individual isoforms with pI values ranging from 3.5 to 5.8
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional copper oxidase and laccase, cf. EC 1.16.3.4
G2H480
UniProt
brenda
isolated from locally prepared compost in the north of Tunisia
FJ560721
GenBank
brenda
best enzyme induction by growth on malt extract, comparison of growth media
-
-
brenda
Phlebia fascicularia
best enzyme induction by growth on malt extract, comparison of growth media
-
-
brenda
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised
-
-
brenda
soil bacterium. The coexistence of this tyrosinase with a laccase in Pseudomonas putida F6 is reported and both activities characterised
-
-
brenda
isolated from a decayed Elaeocarpus sylvestris, identification
-
-
brenda
isolated from a decayed Elaeocarpus sylvestris, identification
-
-
brenda