Cloned (Comment) | Organism |
---|---|
gene lcc1, expression in Aspergillus oryzae as precursor enzyme | Trametes villosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | transient/stopped-flow and steady-state kinetics, modeling | Trametes villosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | blue multi-copper oxidase | Trametes villosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 63000, recombinant enzyme, SDS-PAGE | Trametes villosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes villosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Aspergillus oryzae by gel filtration, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration | Trametes villosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O | reaction mechanism of the reduction of dioxygen to water, second-order rate constant for the reaction with dioxygen, ping-pong mechanism, kinetic model | Trametes villosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoic acid + O2 | - |
Trametes villosa | ? | - |
? | |
4-hydroxyphenylacetic acid + O2 | second-order rate constant for the reaction with dioxygen | Trametes villosa | ? | - |
? | |
guaiacolsulfonic acid + O2 | - |
Trametes villosa | ? | - |
? | |
hydroquinone + O2 | - |
Trametes villosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63000, recombinant enzyme, SDS-PAGE | Trametes villosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trametes villosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 35 | - |
Trametes villosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Trametes villosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trametes villosa | isoelectric focusing | - |
3.5 |