Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 90% inhibition at 0.3 M, completely reversible | Agaricus blazei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
syringaldazine | pH 5.5 | Agaricus blazei | |
0.063 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.5 | Agaricus blazei | |
1.026 | - |
2,6-dimethoxyphenol | pH 5.5 | Agaricus blazei | |
4.31 | - |
guaiacol | pH 5.5 | Agaricus blazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration | Agaricus blazei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus blazei | - |
medicinal mushroom | - |
Purification (Comment) | Organism |
---|---|
native enzyme 8.4fold by ultrafiltration, two steps of anion and one of cation exchange chromatography | Agaricus blazei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Agaricus blazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Agaricus blazei | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Agaricus blazei | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
guaiacol + O2 | - |
Agaricus blazei | ? | - |
? | |
syringaldazine + O2 | - |
Agaricus blazei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Agaricus blazei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
purified laccase is stable at 20°C, pH 7.0 and pH 3.0 | Agaricus blazei |
40 | - |
purified laccase rapidly loses its activity at 40°C | Agaricus blazei |
60 | - |
10 h, almost complete inactivation | Agaricus blazei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
syringaldazine | pH 5.5 | Agaricus blazei | |
15 | - |
2,6-dimethoxyphenol | pH 5.5 | Agaricus blazei | |
21 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.5 | Agaricus blazei | |
159 | - |
guaiacol | pH 5.5 | Agaricus blazei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
substrate-dependent | Agaricus blazei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | purified laccase is stable at 20°C, pH 7.0 and pH 3.0 | Agaricus blazei |
10 | - |
purified laccase rapidly loses its activity at pH 10 | Agaricus blazei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agaricus blazei | isoelectric focusing | - |
4 |