Literature summary for 1.1.3.15 extracted from

Satomura, T.; Hayashi, J.; Sakamoto, H.; Nunoura, T.; Takaki, Y.; Takai, K.; Takami, H.; Ohshima, T.; Sakuraba, H.; Suye, S.I.
D-Lactate electrochemical biosensor prepared by immobilization of thermostabledye-linked D-lactate dehydrogenase from Candidatus Caldiarchaeum subterraneum (2018), J. Biosci. Bioeng., 126, 425-430 .

Search for more literature for 1.1.3.15

Application

Application	Comment	Organism
analysis	construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days	Candidatus Caldarchaeum subterraneum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Candidatus Caldarchaeum subterraneum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
114000	-	gel filtration	Candidatus Caldarchaeum subterraneum

Organism

Organism	UniProt	Comment	Textmining
Candidatus Caldarchaeum subterraneum	E6N788	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol	preferred substrate	Candidatus Caldarchaeum subterraneum	2-oxobutanoate + reduced 2,6-dichlorophenolindophenol	-	?
(R)-lactate + 2,6-dichlorophenolindophenol	preferred substrate	Candidatus Caldarchaeum subterraneum	pyruvate + reduced 2,6-dichlorophenolindophenol	-	?
additional information	no substrates: D-alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate	Candidatus Caldarchaeum subterraneum	?	-	-

Subunits

Subunits	Comment	Organism
dimer	2 * 51000, SDS-PAGE	Candidatus Caldarchaeum subterraneum

Synonyms

Synonyms	Comment	Organism
CSUB_C1080	-	Candidatus Caldarchaeum subterraneum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	30 min, pH 6.0 or pH 8.0, no loss of activity	Candidatus Caldarchaeum subterraneum
70	-	10 min, no loss of acitvity	Candidatus Caldarchaeum subterraneum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Candidatus Caldarchaeum subterraneum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	30 min, 50°C, 40% loss of activity	Candidatus Caldarchaeum subterraneum
6	-	30 min, 50°C, no loss of activity	Candidatus Caldarchaeum subterraneum
8	-	30 min, 50°C, no loss of activity	Candidatus Caldarchaeum subterraneum
9	-	30 min, 50°C, 40% loss of activity	Candidatus Caldarchaeum subterraneum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Candidatus Caldarchaeum subterraneum

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Release 2023.1 (January 2023)