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(R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol
2-oxobutanoate + reduced 2,6-dichlorophenolindophenol
preferred substrate
-
-
?
(R)-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
preferred substrate
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
(S)-lactate + O2
pyruvate + H2O2
2-hydroxy-3-methylvalerate + O2
?
-
A95G-mutant
-
-
?
2-hydroxybutanoate + bromopyruvate + ?
bromolactate + pyruvate + ?
-
transhydrogenation reaction
-
?
2-hydroxybutyrate + O2
?
-
wild-type and A95G-mutant
-
-
?
2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
2-hydroxycaprylate + O2
2-oxocaprylate + H2O2
-
-
-
?
2-hydroxydodecanoate + O2
2-oxododecanoate + H2O2
-
-
-
?
2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
2-hydroxyisovalerate + O2
?
-
A95G-mutant
-
-
?
2-hydroxyoctanoate + 2,6-dichlorophenolindophenol
2-oxo-octanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
r
2-hydroxyoctanoate + O2
2-oxooctanoate + H2O2
-
substrates for isoenzymes HAOX1, HAOX2, preferred substrate for isoenzyme HAOX3
-
?
2-hydroxypalmitate + O2
2-oxopalmitate + H2O2
2-hydroxyvalerate + O2
?
-
wild-type and A95G-mutant
-
-
?
2-mercaptoethanol-glyoxylate adduct + O2
?
-
-
-
-
r
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
coenzyme A-glyoxylate adduct + O2
?
-
-
-
-
r
D-2 -hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
D-lactate + O2
pyruvate + H2O2
DL-2-hydroxy-3-butynoate + O2
2-oxo-3-butynoate + H2O2
-
good substrate, but inactivation after 25 turnovers
-
?
DL-2-hydroxy-3-heptynoate + O2
2-oxo-3-heptynoate + H2O2
-
86% of the activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-3-hexynoate + O2
2-oxo-3-hexynoate + H2O2
-
65% of the activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-3-octynoate + O2
2-oxo-3-octynoate + H2O2
-
70% of the activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-3-pentynoate + O2
2-oxo-3-pentynoate + H2O2
-
2fold higher activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-4-methylmercaptobutyrate + 2,6-dichlorophenolindophenol
2-oxo-4-methylmercaptobutyrate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, low activity for short chain oxidase
-
?
DL-2-hydroxy-4-methylthiobutanoic acid + 2,6-dichlorophenolindophenol
2-oxo-4-methylthiobutanoic acid + reduced 2,6-dichlorophenolindophenol
-
-
-
?
DL-2-hydroxybutyrate + 2,4-dinitrophenylhydrazine
2-oxobutyrate 2,4-dinitrophenylhydrazone + ?
-
low activity
-
?
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol
2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
DL-2-hydroxycaproate + 2,6-dichlorophenolindophenol
2-oxocaproate + reduced 2,6-dichlorophenolindophenol
-
low activity for short chain oxidase, moderate activity for long chain oxidase
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
DL-2-hydroxydecanoate + 2,6-dichlorophenolindophenol
2-oxodecanoate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, traces of activity for short chain oxidase
-
?
DL-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
DL-2-hydroxyisovalerate + 2,4-dinitrophenylhydrazine
2-oxoisovalerate 2,4-dinitrophenylhydrazone + ?
-
very low activity
-
?
DL-2-hydroxyisovalerate + 2,6-dichlorophenolindophenol
2-oxoisovalerate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase, moderate activity for long chain oxidase
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
DL-2-hydroxyoctanoate + 2,6-dichlorophenolindophenol
2-oxooctanoate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, no activity for short chain oxidase
-
?
DL-2-hydroxyvalerate + 2,6-dichlorophenolindophenol
2-oxovalerate + reduced 2,6-dichlorophenolindophenol
-
low activity for short chain oxidase, moderate activity for long chain oxidase
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
DL-3-chlorolactate + O2
3-chloropyruvate + H2O2
DL-3-indolelactate + 2,6-dichlorophenolindophenol
3-indolepyruvate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, no activity for short chain oxidase
-
?
DL-3-indolelactate + O2
3-indolepyruvate + H2O2
-
-
-
?
DL-3-methoxy-4-hydroxymandelate + 2,6-dichlorophenolindophenol
(3-methoxy-4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
DL-alpha-phenyllactate + O2
? + H2O2
-
-
-
-
r
DL-beta-phenyllactate + 2,6-dichlorophenolindophenol
phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase
-
?
DL-glycerate + O2
?
-
-
-
-
r
DL-glycerate + O2
? + H2O2
-
-
-
?
DL-lactate + O2
pyruvate + H2O2
-
-
-
?
DL-mandelate + 2,6-dichlorophenolindophenol
oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
low activity
-
?
DL-mandelate + O2
phenylglyoxylic acid + H2O2
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
DL-methionine + O2
? + H2O2
-
-
-
?
DL-p-hydroxy-beta-phenyllactate + 2,6-dichlorophenolindophenol
(4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase
-
?
DL-p-hydroxymandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
DL-phenyllactate + O2
phenylpyruvate + H2O2
DL-vinylglycolate + O2
2-oxo-3-butenoic acid + H2O2
-
90% of the activity compared to DL-2-hydroxybutyrate
-
?
glycolate + 2,4-dinitrophenylhydrazine
glyoxylate 2,4-dinitrophenylhydrazone + ?
-
best substrate tested
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
glycolate + acceptor
glyoxylate + reduced acceptor
glycolate + ferricyanide
glyoxylate + ferrocyanide
glycolate + O2
glyoxylate + H2O2
glycolate + phenazine methosulfate
glyoxylate + reduced phenazine methosulfate
-
-
-
?
glycolate + phenylhydrazine
glyoxylate + phenylhydrazone
-
-
-
?
glyoxalate + O2
oxalate + H2O2
-
-
-
-
?
glyoxylate + 2,6-dichlorophenolindophenol
?
-
-
-
-
?
glyoxylate + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
glyoxylate + ferricyanide
? + ferrocyanide
-
-
-
?
glyoxylate thiohemiacetals + O2
? + H2O2
-
possible natural substrates, i.e. glyoxylate thiohemiacetals of coemzyme A, D-phosphopantetheine, D-pantetheine, N-acetylcysteamine, 2-mercaptoethanol, DL-dihydrolipoate, propane-1,3-dithiol
-
?
homoserine + O2
? + H2O2
-
traces of activity
-
?
isoleucic acid + O2
? + H2O2
-
-
-
?
L-2-hydroxy octanoate + O2
2-oxo-octanoate + H2O2
-
-
-
?
L-2-hydroxy palmitate + O2
2-oxo-palmitate + H2O2
-
-
-
?
L-2-hydroxy-4-methylthiobutanoic acid + O2
3-(methylthio)propanoate + HCO3-
-
oxidative decarboxylation
-
?
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol
3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
L-2-hydroxyisocaproate + 2,4-dinitrophenylhydrazine
2-oxoisocaproate 2,4-dinitrophenylhydrazone + ?
-
-
-
?
L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol
2-oxoisocaproate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
L-2-hydroxyisocaproate + O2
4-methyl-2-oxopentanoate + H2O2
-
100% activity with long chain oxidase, 32% activity with short chain oxidase
-
-
r
L-2-hydroxyphenyllactate + O2
? + H2O2
L-4-chloromandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-fluoromandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-methoxymandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-methylmandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-nitromandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-trifluoromethylmandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-alanine + O2
? + H2O2
-
low activity
-
?
L-alpha-hydroxy-isovalerate + O2
3-methyl-2-oxobutanoate + H2O2
-
-
-
-
r
L-alpha-hydroxyphenyllactate + O2
? + H2O2
-
-
-
-
r
L-isoleucine + O2
? + H2O2
-
low activity
-
?
L-lactate + 2,4-dinitrophenylhydrazine
pyruvate 2,4-dinitrophenylhydrazone + ?
-
very low activity
-
?
L-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
L-lactate + O2
pyruvate + H2O2
L-lactate + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
L-lactate + phenylhydrazine
pyruvate + phenylhydrazone
-
-
-
?
L-leucine + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lysine + O2
? + H2O2
-
-
-
?
L-mandelate + 2,6-dichlorophenolindophenol
?
-
-
-
-
?
L-mandelate + 2,6-dichlorophenolindophenol
oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
L-mandelate + O2
? + H2O2
-
-
-
?
L-methionine + O2
? + H2O2
L-phenylalanine + O2
? + H2O2
-
-
-
?
L-phenyllactate + 2,6-dichlorophenolindophenol
phenylpyruvate + reduced 2,6-dichlorophenolindophenol
L-tryptophan + O2
? + H2O2
L-tyrosine + O2
? + H2O2
-
-
-
?
L-valine + O2
? + H2O2
-
low activity
-
?
lactate + O2
pyruvate + H2O2
mandelate + O2
phenylpyruvate + H2O2
oxidation of an L-2-hydroxy acid to a 2-oxoacid, model for the binding of L-mandelate into the active site, overview
-
-
?
N-acetylcysteamine-glyoxylate adduct + O2
?
-
-
-
-
r
pantetheine-glyoxylate adduct + O2
?
-
-
-
-
r
propane-1,3-dithiol-glyoxylate adduct + O2
?
-
-
-
-
r
thiol-glyoxylate adducts + O2
an oxalyl thioester + H2O2
-
may be the physiological substrates
-
?
valic acid + O2
? + H2O2
-
-
-
?
additional information
?
-
(S)-lactate + O2
acetate + CO2 + H2O
-
-
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
-
-
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
-
-
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
-
glucose-repressible lactate oxidase is likely responsible for H2O2 production
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
no decarboxylation
-
?
(S)-lactate + O2
pyruvate + H2O2
-
no decarboxylation
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
-
?
2-hydroxypalmitate + O2
2-oxopalmitate + H2O2
-
-
-
?
2-hydroxypalmitate + O2
2-oxopalmitate + H2O2
-
substrates for isoenzymes HAOX1, HAOX2
-
1.1.3.15 (S)-2-hydroxy-acid oxidase
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