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Literature summary for 1.1.1.B20 extracted from

  • Jang, J.W.; Jung, H.M.; Im, D.K.; Jung, M.Y.; Oh, M.K.
    Pathway engineering of Enterobacter aerogenes to improve acetoin production by reducing by-products formation (2017), Enzyme Microb. Technol., 106, 114-118 .
    View publication on PubMed
Search for more literature for 1.1.1.B20

Protein Variants

Protein Variants Comment Organism
additional information Enterobacter aerogenes is metabolically engineered for acetoin production. To remove the pathway enzymes that catalyze the formation of by-products, the three genes encoding a lactate dehydrogenase (ldhA) and two 2,3-butanediol dehydrogenases (budC, and dhaD), respectively, are deleted from the genome. The acetoin production is higher under highly aerobic conditions. An extracellular glucose oxidative pathway in Enterobacter aerogenes is activated under the aerobic conditions, resulting in the accumulation of 2-ketogluconate. To decrease the accumulation of this by-product, the gene encoding a glucose dehydrogenase (gcd) is also deleted. The resulting strain does not produce 2-ketogluconate but produces significant amounts of acetoin, with concentration reaching 71.7 g/l with 2.87 g/l/h productivity in fed-batch fermentation. The resulting strains are EMY-01 (DELTAldhA), EJW-0 (DELTAldhA-DELTAbudC), EJW-02 (DELTAldhA-DELTAbudC-DELTAdhaD), and EJW-03 (DELTAldhA-DELTAbudC-DELTAdhaD-DELTAgcd), evaluation for acetoin production, overview Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-butane-2,3-diol + NAD+ Klebsiella aerogenes
-
 (3R)-acetoin + NADH + H+
-
 r
(2R,3S)-butane-2,3-diol + NAD+ Klebsiella aerogenes KCTC 2190
-
 (3R)-acetoin + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes A0A0F1TDA0 Enterobacter aerogenes
-
Klebsiella aerogenes KCTC 2190 A0A0F1TDA0 Enterobacter aerogenes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-butane-2,3-diol + NAD+
-
 Klebsiella aerogenes (3R)-acetoin + NADH + H+
-
 r
(2R,3S)-butane-2,3-diol + NAD+
-
 Klebsiella aerogenes KCTC 2190 (3R)-acetoin + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenases
-
 Klebsiella aerogenes
budC
-
 Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Klebsiella aerogenes
NADH
-
 Klebsiella aerogenes

General Information

General Information Comment Organism
malfunction deletion of budC causes redox imbalance towards NADH Klebsiella aerogenes
physiological function acetoin can be converted to 2,3-butanediol by 2,3-butanediol dehydrogenase (budC) with consumption of NADH Klebsiella aerogenes