KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0683 | - |
NAD+ | pH 8.0, 70°C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44300 | - |
2 * 44300, calculated from sequence | Pyrococcus furiosus |
70000 | - |
analytical ultracentrifugation | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U488 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | low activity with NADP+. Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ | Pyrococcus furiosus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 44300, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
NAD-IDH | - |
Pyrococcus furiosus |
PF0202 | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
above | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal denaturation is an irreversible process | Pyrococcus furiosus |
104 | - |
the Tm-value is 103.7°C | Pyrococcus furiosus |