Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Azospirillum brasilense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | slight inhibition at 2 mM | Azospirillum brasilense | |
acetyl phosphate | slight inhibition at 2 mM | Azospirillum brasilense | |
acetyl-CoA | enzyme inhibition by acetyl-CoA is relieved by increasing CoASH concentrations | Azospirillum brasilense | |
ADP | slight inhibition at 2 mM | Azospirillum brasilense | |
ATP | slight inhibition at 2 mM | Azospirillum brasilense | |
cAMP | slight inhibition at 1 mM | Azospirillum brasilense | |
CoA | slight inhibition at 1 mM | Azospirillum brasilense | |
glutamine | slight inhibition at 5 mM | Azospirillum brasilense | |
phosphoenolpyruvate | slight inhibition at 1 mM | Azospirillum brasilense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.157 | - |
NADP+ | at pH 8.0 and 30°C | Azospirillum brasilense | |
2.9 | - |
(S)-malate | in the presence of 1 mM NADP+, at pH 8.0 and 30°C | Azospirillum brasilense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the enzyme is more active in buffer containing 100 mM K+ in comparison to 100 mM Na+ | Azospirillum brasilense | |
Mg2+ | activates | Azospirillum brasilense | |
Mn2+ | activates, 5 mM used in assay conditions | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
710000 | - |
gel filtration | Azospirillum brasilense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Azospirillum brasilense | the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Azospirillum brasilense FP2 | the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions | pyruvate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Azospirillum brasilense FP2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heparin column chromatography | Azospirillum brasilense |
His-tagged enzyme is purified by HiTrap chelating Ni2+ column chromatography, untagged enzyme is partially purified by DEAE column chromatography | Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Azospirillum brasilense | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions | Azospirillum brasilense | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Azospirillum brasilense FP2 | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions | Azospirillum brasilense FP2 | pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | the reverse reaction is very slow | Azospirillum brasilense | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | the reverse reaction is very slow | Azospirillum brasilense FP2 | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 83000, SDS-PAGE | Azospirillum brasilense |
Synonyms | Comment | Organism |
---|---|---|
MaeB1 | - |
Azospirillum brasilense |
NADP-dependent malic enzyme | - |
Azospirillum brasilense |
TME | - |
Azospirillum brasilense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
pyruvate | at pH 8.0 and 30°C | Azospirillum brasilense | |
327 | - |
(S)-malate | at pH 8.0 and 30°C | Azospirillum brasilense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme preferentially uses NADP+ as co-factor | Azospirillum brasilense | |
NADPH | - |
Azospirillum brasilense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.139 | - |
acetyl-CoA | at pH 8.0 and 30°C | Azospirillum brasilense |